PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets

Asensio, Luis; González, Isabel; Rojas, María; Garcı́a, Teresa; Martı́n, Rosario

doi:10.1016/j.foodcont.2008.09.006

Cited by 25 publications

(11 citation statements)

References 15 publications

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“…Considering that seafood mislabeling has been widely reported throughout the world (Jacquet and Pauly, 2008;Asensio et al, 2009;Cawthorn et al, 2011;Garcia-Vazquez et al, 2011;Hanner et al, 2011;Miller et al, 2012;Cox et al, 2013;Di Pinto et al, 2013) and that the authentication of food components is one of the key issues in food quality, the aim of this study was to investigate the prevalence of mislabeling among fish fillet products from markets and supermarkets located in Apulia (SE Italy) using DNA barcoding, the sequencing of the mitochondrial reference marker gene cytochrome oxidase subunit 1 (CO1) (Hebert et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Species identification in fish fillet products using DNA barcoding

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Species identification in fish fillet products using DNA barcoding

et al. 2015

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“…The molecular biology techniques provide a robust and valuable tool for identification of the plant or animal species present in a foodstuff (Peres, Barlet, Loiseau, & Montet, 2007). Recently, several mitochondrial or nuclear genes were extensively studied in many fishery products to reach the goal of the species differentiation, for instance of fish (Asensio, Gonzalez, Rojas, Garcia, & Martin, 2009;Comi, Iacumin, Rantsiou, Cantoni, & Cocolin, 2005;Lin & Hwang, 2008;Pardo & Perez, 2004;Rea, Storani, Mascaro, Stocchi, & Loschi, 2009;Tsai, Chen, Hsieh, Lin, & Hwang, 2009;Zhang, Huang, Cai, & Huang, 2006), shrimp (Pascoal, Barros-Velazquez, & Cepeda, 2008) and shellfish (Fernandez-Tajes, Freire, & Mendez, 2010;Fernandez-Tajes & Mendez, 2007). However, works on the identification of sea cucumber species in food products are very limited (Wen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetic identification of global commercial sea cucumber species on the basis of mitochondrial DNA sequences

Wen

Zhang

et al. 2011

Food Control

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“…Molecular markers used in the multiplex fingerprinting technique for the authentication, control, and inspection of fishery stocks are based on the design of specific oligonucleotides and markers derived from the sequencebased amplification of the 5S, 12S, 18S (Asensio, 2008, Asensio et al, 2009, 16S (Ulrich et al, 2013), Cyt b (Melo Palmeira et al, 2013), and COI genes , as well as fragment length polymorphisms, RFLPs (Paine et al 2007;Torres et al 2013) or even nuclear markers, such as ITS 2 (Feldheim et al, 2010), SNPs (Torres et al, 2013) and microsatellites (Rodrigues et al, 2011), and realtime PCR techniques (Trotta et al, 2005). The COI gene is a highly effective and reliable tool for the diagnosis of the authenticity of fishery products (Veneza et al, 2014), and the multiplex PCR technique has proven especially effective for the detection of endangered species De-Franco et al, 2010;Alexandre de-Franco et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

et al. 2016

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The Atlantic goliath grouper, Epinephelus itajara, is a critically endangered species, threatened by illegal fishing and the destruction of its habitats. A number of other closely related grouper species found in the western Atlantic are also fished intensively. While some countries apply rigorous legislation, illegal harvesting followed by the falsification of fish products, which impedes the correct identification of the species, is a common practice, allowing the catch to be marketed as a different grouper species. In this case, molecular techniques represent an important tool for the monitoring and regulation of fishery practices, and are essential for the forensic identification of a number of different species. In the present study, species-specific primers were developed for the Cytochrome Oxidase subunit I gene, which were applied in a multiplex PCR for the simultaneous identification of nine different species of Epinephelidae: Epinephelus itajara, E. quinquefasciatus, E. morio, Hyporthodus flavolimbatus, H. niveatus, Mycteroperca acutirostris, M. bonaci, M. marginata, and M. microlepis.Multiplex PCR is a rapid, reliable and cost-effective procedure for the identification of commercially-valuable endangered fish species, and may represent a valuable tool for the regulation and sustainable management of fishery resources.O mero, Epinephelus itajara, encontra-se criticamente ameaçado, resultado da pesca ilegal e destruição dos habitas. Filogeneticamente relacionadas a este táxon encontram-se garoupas que atualmente são intensamente pescadas no Atlântico Oeste. Apesar de leis mais restritivas aplicadas em alguns países, a captura ilegal com a descaracterização morfológica é uma prática comum que impossibilita a identificação correta da espécie permitindo que seja comercializada como garoupas, badejos ou chernes. A este respeito, técnicas moleculares representam ferramentas importantes para o monitoramento e fiscalização da pesca, provando ser essencial, na identificação forense de diversas espécies. Primers espécie-específicos foram desenvolvidos com base no gene Citocromo Oxidase subunidade I que aplicados em PCR-Multiplex possibilitam a identificação simultânea de nove espécies Epinephelidae: Epinephelus itajara, E. quinquefasciatus, E. morio, Hyporthodus flavolimbatus, H. niveatus, Mycteroperca acutirostris, M. bonaci, M. marginata e M. microlepis. A identificação via PCR multiplex de espécies de peixes ameaçadas e comercialmente importantes é um método rápido, prático, seguro e de baixo custo, que poderá ser útil o controle do uso e manejo sustentável de recursos pesqueiros.

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PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets

Cited by 25 publications

References 15 publications

Species identification in fish fillet products using DNA barcoding

Species identification in fish fillet products using DNA barcoding

Genetic identification of global commercial sea cucumber species on the basis of mitochondrial DNA sequences

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

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