PCR-Based Identification of Bacteria Associated with Endodontic Infections

Fouad, Ashraf F.; Barry, Jody; Caimano, Melissa J.; Clawson, Marion; Zhu, Qiang; Carver, Rachaele; Hazlett, Karsten R. O.; Radolf, Justin D.

doi:10.1128/jcm.40.9.3223-3231.2002

Cited by 229 publications

(214 citation statements)

References 58 publications

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“…Many anaerobic microorganisms are difficult to culture and identify precisely; thus, molecular methods have been used to identify microorganisms from endodontic infections. 6 The aim of this study was to detect the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii in the PCs and RCs of primary teeth, as well as to compare the prevalence of these species in both environments.…”

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confidence: 99%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

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An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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“…By using the dried paper points samples were drawn from the root canal infected cases. 9 Simultaneously their clinical history was also noted.…”

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confidence: 99%

Effect of Garcinia Imberti Bourd Against Candida Isolates in Rootcanal Infection

Ramkumar¹,

Sivakumar²,

Angelo³

2017

JYP

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Background: Many research works are being carried out in preparing intracanal medicaments using herbal plants since it is safe as well as containing a better therapeutic value. The present study was designed to evaluate the anticandidal properties of the leaves of Garcinia imberti bourd in associated with the root canal infection. Method: Totally 94 root canal infected samples were collected and their previous history also recorded. Samples yielded Candida isolates were screened through drug resistant study. Multi drug-resistant Candida albicans was isolated and characterized by 18S rDNA sequencing. An antifungal activity of the extracts of the leaves of G.imberti bourd was performed by disc diffusion method. Using Silica-60 column active fraction of the methanol extract was partially purified and was analyzed by Gas Chromatography-Mass Spectrophotometry (GC-MS). Prepare intracanal medicament with methanol extract of G.imberti bourd and was tested with multidrug-resistant C.albicans (Ca-09) under in-vitro conditions using decoronated teeth. Efficacy also compared with chlorohexidine. Result: A total of 48 Candida sp. were isolated and screened for antibiotic susceptible test. Among them isolate Ca -09 showed multiple antibiotic resistant. Crude methanol fraction of G. imberti bourd leaf extract, as well as the partially purified compound of this extract, showed better anticandidal activity. 0.01g of methanol extract with 2% methyl cellulose gel showed significant reduction (63%) of C.albicans in in-vitro intracanal treatment. Conclusion: Methanol extract of Garcinia imberti bourd leaf contains glycerine, which can be used in preparing the suitable intracanal medicament for the treatment of root canal infection.

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“…흑색집락형성 Gram음성 혐기성 간균 중에서도 P. endodontalis와 P. gingivalis는 동통, 종창, 농 등과 같은 임상증상, 급성감염과 관련이 깊은 세균종이라고 보고되어 왔다 5,[8][9][10][12][13][14][15][16][17] . 그러나 최근 16S rDNA(RNA)gene-directed PCR의 결과에 따르면 이들 세균의 관련성에 관한 의미 가 축소되고 있는 경향이 나타나고 있다 34,38,[42][43][44][45] . 이 연구에 서도 누공이 형성되지 않고 동통이나 종창을 보인 12개 감 염근관에서 P. endodontalis나 P. gingivalis가 나타난 경 우는 11개로 91.7%였고, 둘 다 나오는 경우는 4개로 33.3%였다 (Table 4 …”

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Distribution of oral pathogens in infections of endodontic origin

Kim

Choi

Park

et al. 2003

J Korean Acad Conserv Dent

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It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 16S rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula.1. In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%). 2. Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms. 3. Of 36 infected root canals, Pi and Pn were found in 4 (13.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%. 4. One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%. 5. Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms. 6. Td was detected in the teeth regardless of the presence or absence of symptoms. High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.K Ke ey y w wo or rd ds s : PCR, BPB, infection, clinical symptom, periapical lesion, root canal ABSTRACT 치료의 성공을 좌우한다

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PCR-Based Identification of Bacteria Associated with Endodontic Infections

Cited by 229 publications

References 58 publications

An investigation of the presence of specific anaerobic species in necrotic primary teeth

An investigation of the presence of specific anaerobic species in necrotic primary teeth

Effect of Garcinia Imberti Bourd Against Candida Isolates in Rootcanal Infection

Distribution of oral pathogens in infections of endodontic origin

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