PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus

Abstract

Objective: The objective of the study was to use polymerase chain reaction (PCR) to examine and compare the genotype distribution of human papillomavirus (HPV) in oral lichen planus (OLP). Materials and Methods: Deoxyribonucleic acid (DNA) was extracted from 20 OLP biopsy specimens. Conventional PCR assay employing consensus HPV primers was used to identify HPV DNA. Positive PCR samples were then subjected to PCR assay with HPV type-specific primers. Results: Out of the total 20 OLP specimens evaluated, eight samples (40%) were positive for HPV. Females had a 41.7% higher HPV-positive rate than males. The most common type in the HPV type-specific PCR assay was HPV-18 (75%), which is a high-risk type of HPV linked to malignant diseases. The erosive kind of OLP had the greatest percentage of HPV positives (50%). Conclusion: The present study confirms the detection of HPV in OLP lesions, as determined by PCR-coupled HPV gene sequencing, as well as its likely mechanism of malignant transformation.

The association between high‐risk human papillomavirus and oral lichen planus

“…The type of HPV detected in OLP lesions also seems to be geographically dependent, although high‐risk HPVs, especially HPV‐18, was the predominant subtype in most of the studies (present study; Kaewmaneenuan et al, 2021; T. Liu et al, 2018; Sameera et al, 2019; Vijayan et al, 2022). Although the correlation between HPV and OLP subtype is debatable, most of the studies with significant results report that HPV detection in erosive‐atrophic subtype is well above that of non‐erosive‐atrophic subtype of OLP (Farhadi et al, 2020; Kaewmaneenuan et al, 2021; Vijayan et al, 2022).…”

“…There are still conflicting results about the association between HPV infection and OLP; while some report the high detection rate (DR) of HPV virus in OLP lesions or the significant difference in the frequency of the virus between OLP and normal mucosa (Della Vella et al, 2021;Farhadi et al, 2020;Kaewmaneenuan et al, 2021;T. Liu et al, 2018;Poulopoulos et al, 2017;Sameera et al, 2019;Vijayan et al, 2022), others report the low detection rate of the virus or the nonsignificant difference in the frequency of the virus between OLP and normal mucosa (Bar et al, 2021;Gomez-Armayones et al, 2019;Illeperuma et al, 2021;Jabar, 2017;Nafarzadeh et al, 2017;Panzarella et al, 2017;Zare et al, 2022). According to 2017; Zare et al, 2022).…”

“…Liu et al, 2018;Sameera et al, 2019;Vijayan et al, 2022). Although the correlation between HPV and OLP subtype is debatable, most of the studies with significant results report that HPV detection in erosiveatrophic subtype is well above that of non-erosive-atrophic subtype of OLP (Farhadi et al, 2020;Kaewmaneenuan et al, 2021;Vijayan et al, 2022).…”

“…Overall, the detection rate of HPV varied from 0%–86.6% in OLP samples and 0%–28% in control (normal oral mucosa) samples in these studies. There are still conflicting results about the association between HPV infection and OLP; while some report the high detection rate (DR) of HPV virus in OLP lesions or the significant difference in the frequency of the virus between OLP and normal mucosa (Della Vella et al, 2021 ; Farhadi et al, 2020 ; Kaewmaneenuan et al, 2021 ; T. Liu et al, 2018 ; Poulopoulos et al, 2017 ; Sameera et al, 2019 ; Vijayan et al, 2022 ), others report the low detection rate of the virus or the nonsignificant difference in the frequency of the virus between OLP and normal mucosa (Bar et al, 2021 ; Gomez‐Armayones et al, 2019 ; Illeperuma et al, 2021 ; Jabar, 2017 ; Nafarzadeh et al, 2017 ; Panzarella et al, 2017 ; Zare et al, 2022 ). According to Table 6 , the association of HPV and OLP varied significantly by geographic population; even in different area of the same country, this relationship is associated with varied results (present study; Farhadi et al, 2020 ; Nafarzadeh et al, 2017 ; Zare et al, 2022 ).…”

Характеристика Клітинного Та Видового Складу Мікрофлори Слизової Оболонки Порожнини Рота У Хворих На Червоний Плоский Лишай

“…Це підтверджується системно -лейкоцитозом, еозинофілією, підвищенням ШОЕ, рівня цитотоксичної субпопуляції лімфоцитів, концентрації IgG, IgM, циркулюючих імунокомплексів, локально -підвищенням кількості поліморфноядерних і мононуклеарних клітин, їх зруйнованих фрагментів, прозапального TNF-α і всіх відомих імуноглобулінів. Разом з тим, спостерігається дисбаланс у показниках імунного статусу з ознаками розвитку вторинного імунодефіциту та порушенням процесів регенерації -зниження в крові кількості загальних лімфоцитів і їх хелперної субпопуляції, концентрації IgA, локальнозниження вмісту епітеліальних клітин і протизапального цитокіну IL-4 [11,12].…”