PCR Based Detection of Adulteration in the Market Samples of Turmeric Powder

Sasikumar, B.; Syamkumar, S.; Remya, R.; Zachariah, T. J.

doi:10.1081/lfbt-200035022

Cited by 18 publications

(19 citation statements)

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“…Therefore, detecting adulteration is a prerequisite to food safety also. Adulteration has been reported in commercially sold turmeric powder, adversely affecting the quality of the product and its trade (Dhanya et al, 2011;Sasikumar et al, 2005). Plant-based adulterants or fillers include wild species of Curcuma, namely C. zedoaria, starch from cheaper sources such as cassava, and sawdust (Dhanya & Sasikumar, 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Parvathy

Swetha

Sheeja

et al. 2015

Pharmaceutical Biology

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Context: In its powdered form, turmeric [Curcuma longa L. (Zingiberaceae)], a spice of medical importance, is often adulterated lowering its quality. Objective: The study sought to detect plant-based adulterants in traded turmeric powder using DNA barcoding. Materials and methods: Accessions of Curcuma longa L., Curcuma zedoaria Rosc. (Zingiberaceae), and cassava starch served as reference samples. Three barcoding loci, namely ITS, rbcL, and matK, were used for PCR amplification of the reference samples and commercial samples representing 10 different companies. PCR success rate, sequencing efficiency, occurrence of SNPs, and BLAST analysis were used to assess the potential of the barcoding loci in authenticating the traded samples of turmeric. Results: The PCR and sequencing success of the loci rbcL and ITS were found to be 100%, whereas matK showed no amplification. ITS proved to be the ideal locus because it showed greater variability than rbcL in discriminating the Curcuma species. The presence of C. zedoaria could be detected in one of the samples whereas cassava starch, wheat, barley, and rye in other two samples although the label claimed nothing other than turmeric powder in the samples. Discussion and conclusion: Unlabeled materials in turmeric powder are considered as adulterants or fillers, added to increase the bulk weight and starch content of the commodity for economic gains. These adulterants pose potential health hazards to consumers who are allergic to these plants, lowering the product's medicinal value and belying the claim that the product is gluten free. The study proved DNA barcoding as an efficient tool for testing the integrity and the authenticity of commercial products of turmeric.

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Section: Introductionmentioning

confidence: 99%

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Parvathy

Swetha

Sheeja

et al. 2015

Pharmaceutical Biology

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“…Curcumin content varies in the range of 0.3% to 8.6% [47,[51][52][53][54], due to nutrients and acidity content in soil [55,56], fertilizer, soil type and cultivar [57][58][59]. Due to the isolation of curcumin from turmeric for medicinal and cosmetic purposes, it is adulterated with color dyes as a substitute for curcumin [60]. Metanil yellow (C 18 H 14 N 3 NaO 3 S), a yellow color toxic azo dye, is added to turmeric for brighter color and appealing appearance [32].…”

Section: Application To Authenticate Turmeric Powdermentioning

confidence: 99%

A 1064 nm Dispersive Raman Spectral Imaging System for Food Safety and Quality Evaluation

et al. 2018

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Raman spectral imaging is an effective method to analyze and evaluate the chemical composition and structure of a sample, and has many applications for food safety and quality research. This study developed a 1064 nm dispersive Raman spectral imaging system for surface and subsurface analysis of food samples. A 1064 nm laser module is used for sample excitation. A bifurcated optical fiber coupled with Raman probe is used to focus excitation laser on the sample and carry scattering signal to the spectrograph. A high throughput volume phase grating disperses the incoming Raman signal. A 512 pixels Indium-Gallium-Arsenide (InGaAs) detector receives the dispersed light signal. A motorized positioning table moves the sample in two-axis directions, accumulating hyperspectral image of the sample by the point-scan method. An interface software was developed in-house for parameterization, data acquisition, and data transfer. The system was spectrally calibrated using naphthalene and polystyrene. It has the Raman shift range of 142 to 1820 cm −1 , the spectral resolution of 12 cm −1 at full width half maximum (FWHM). The spatial resolution of the system was evaluated using a standard resolution glass test chart. It has the spatial resolution of 0.1 mm. The application of the system was demonstrated by surface and subsurface detection of metanil yellow contamination in turmeric powder. Results indicate that the 1064 nm dispersive Raman spectral imaging system is a useful tool for food safety and quality evaluation.

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“…Molecular marker analysis has been widely used for differentiation of a large number of medicinal plant species from their close relatives or adulterants (Shaw and But, 1995;Cheng et al, 1997Cheng et al, , 2000Kochieva, 1999;Sasikumar et al, 2004). Scheef et al (2003) identifi ed two RAPDbased species-specifi c markers for two Agrostis species (A. capillaries -colonial type and A. palustris -creeping type) and subsequently developed (SCAR) markers, which were tested in 17 cultivars belonging to four species.…”

Section: Discussionmentioning

confidence: 99%

Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

Senapati

Aparajita

Rout

2011

Zeitschrift Für Naturforschung C

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Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identifi cation of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. In the present study, attempts were made to identify species-diagnostic markers for ten Phyllanthus species using the inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) fi ngerprinting method. PCR amplifi cation using seven ISSR primers resulted in signifi cant polymorphism among the populations from different species. P. angustifolius and P. urinaria showed monomorphic frequency of maximum (63.88%) and minimum (20.64%), respectively. Seventeen species-diagnostic markers were identifi ed for seven species (P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius) while no marker was detected for P. reticulatus, P. nivosus, and P. virgulatus. A maximum of six species-diagnostic markers were identifi ed for P. acidus and a minimum of only one of 755 bp was available for P. amarus. Among the seventeen markers, nine were present in all individuals of particular species. The speciesspecifi c differences in fragment numbers and sizes could be used as diagnostic markers to distinguish the Phyllanthus species quickly.

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PCR Based Detection of Adulteration in the Market Samples of Turmeric Powder

Cited by 18 publications

References 0 publications

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Detection of plant-based adulterants in turmeric powder using DNA barcoding

A 1064 nm Dispersive Raman Spectral Imaging System for Food Safety and Quality Evaluation

Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

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