PCL–PU composite vascular scaffold production for vascular tissue engineering: Attachment, proliferation and bioactivity of human vascular endothelial cells

Williamson, Matthew R.; Black, Richard A.; Kielty, Cay M.

doi:10.1016/j.biomaterials.2006.02.025

Cited by 126 publications

(132 citation statements)

References 22 publications

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“…-Real Time Polymerase Chain Reduction (PCR) for determining the abundance of different RNA molecules within the cells in order to assess regulation of gene expression [137,[139][140][141].…”

Section: Materials and Methods) A Similar Commercial Solution Is Altmentioning

confidence: 99%

“…-Western Blotting for semiquantative detection of specific protein expression [139,141,142]. After cell detachment from ES scaffold, proteins are extracted from cells and they are separated by using gel elecrophoresis.…”

Section: Materials and Methods) A Similar Commercial Solution Is Altmentioning

confidence: 99%

“…The process was invented at the beginning of 1900 by Cooley and Morton [136,137] and it was mainly developed thanks to the contribution of Formhals' patents [138][139][140][141][142] for textile applications. However, only at the beginning of this century ES began to be used for scaffold fabrication [143][144][145][146][147] and nowadays it is considered a powerful technique that is employed to this aim by many researchers all around the world.…”

Section: Electrospinningmentioning

confidence: 99%

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Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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Section: Materials and Methods) A Similar Commercial Solution Is Altmentioning

confidence: 99%

Section: Materials and Methods) A Similar Commercial Solution Is Altmentioning

confidence: 99%

Section: Electrospinningmentioning

confidence: 99%

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Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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“…Nitric oxide is produced by many cell types and is an important effector molecule for several processes [34,35]. The NO plasma quantification after 2 weeks of PVDF composite contact with subcutaneous implants is shown in Fig.…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

Costa

Ribeiro

Lopes

et al. 2012

J Mater Sci: Mater Med

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Electroactive materials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-weekold C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.

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“…A few studies have shown that aligned electrospun scaffolds are able to provide contact guidance to cultured cells, resulting in an elongation and alignment of cells along the axes of the fibers [2][3][4]. The majority of the studies revolve around the evaluation of cell morphological changes, whilst some assess the preservation of cell phenotype via gene or protein expression analysis [5,6]. Quantitative evaluation of changes in cellular function under the influence of topographic cues provided by electrospun fibers is still limited [3,7,8].…”

Section: Introductionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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PCL–PU composite vascular scaffold production for vascular tissue engineering: Attachment, proliferation and bioactivity of human vascular endothelial cells

Cited by 126 publications

References 22 publications

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

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