Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

Abstract

Electroactive materials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-weekold C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.

Processing and size range separation of pristine and magnetic poly( l -lactic acid) based microspheres for biomedical applications

“…PLLA is a biocompatible polymer, but the CoFe 2 O 4 particles are cytotoxic [46]. On the other hand, these particles can be ''encapsulated" in a polymer in order to achieve a biocompatible magnetic composite [47]. In this way, one of the objectives in this study was to verify if these magnetic particles were properly encapsulated in the PLLA spheres.…”

Effect of filler content on morphology and physical–chemical characteristics of poly(vinylidene fluoride)/NaY zeolite-filled membranes

“…Further, the control of some characteristics of the composites such as polymer porosity or zeolite ionic charge affects the surface and chemical interaction of the composites with other materials, which is of critical importance in areas such as battery separators [19], filtration [20] and tissue engineering, by tailoring cell-material surface interactions [21]. One of the most efficient ways to increase the surface area of composites consists on the production of microfibers by electrospinning [22], in which the modification of the processing parameters lead to variations in electrospun fiber morphology, orientation and diameter [23].…”

“…It has been proven the biocompatibility of PVDF/NaY composite films [21], which also promotes cell response. Further, zeolite A induces proliferation and differentiation of human osteoblast cells [38] and increases bone cell proliferation and extracellular matrix production [39].…”

“…Finally, despite the cell viability has been proven in the composite films [21], it is important to test this issue on the electrospun membranes as the different preparation conditions, in particular the different solvent evaporation conditions that could lead, for example, to solvent encapsulation in the zeolite fillers, could inhibit cell viability. Figure 8 shows the viability of the attached MC3T3-E1 cells on PVDF polymer and polymer composite films and membranes after 72 h days of cell culture.…”

Poly(vinylidene fluoride-trifluoroethylene)/NAY zeolite hybrid membranes as a drug release platform applied to ibuprofen release

“…They are biocompatible [14] and have been used for a larger range of applications including gas storage, filtration and catalytic processes, incorporation of additives for textile applications, among others [15]. Their ability to absorb molecules is related to the zeolite channel geometry and the chemical nature of the ions present in the zeolite structure [13,16].…”