PCB Technology-Based Electrochemiluminescence Microfluidic Device for Low-Cost Portable Analytical Systems

Pittet, Patrick; Lu, Guo‐Neng; Galvan, Jean‐Marc; Ferrigno, Rosaria; Blum, Loı̈c J.; Leca‐Bouvier, Béatrice D.

doi:10.1109/jsen.2008.918994

Cited by 23 publications

(16 citation statements)

References 20 publications

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“…PCB electrodes are more advantageous than thin-film electrodes in terms of cost, design features, fabrication, large-scale production, reliability, and electronic integration. 29 Using PCB technology, it is possible to design low-resistance electrodes on insulator substrates which can withstand larger currents than a thin-film electrode. 29 After performing the necessary washing step as described in the experimental section, oxygen plasma treatment was performed to modify the gold substrate into a hydrophilic one for enhancing immobilization of graphene oxide.…”

Section: Au-pcb Wementioning

confidence: 99%

“…29 Using PCB technology, it is possible to design low-resistance electrodes on insulator substrates which can withstand larger currents than a thin-film electrode. 29 After performing the necessary washing step as described in the experimental section, oxygen plasma treatment was performed to modify the gold substrate into a hydrophilic one for enhancing immobilization of graphene oxide. In general, graphene oxide possesses chemically reactive oxygen functional groups at the edges and basal planes of a sheet-like nanostructure, making it suitable for several types of chemical functionalization.…”

Section: Au-pcb Wementioning

confidence: 99%

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Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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Abstract:A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 µA mM. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H 2 O 2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.

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Section: Au-pcb Wementioning

confidence: 99%

Section: Au-pcb Wementioning

confidence: 99%

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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“…However, none of these detection methods can satisfy all requirements for the ideal detection of miRNAs: rapid, label-free, low reagent consumption and easy-to-operate. To meet those requirements, microfluidic systems combined with Electrochemiluminescence detection could be a compelling choice 16,17 .…”

Section: Introductionmentioning

confidence: 99%

Amplification-free detection of miRNA via an ECL chips system

Liu

Zhou

2012

SPIE Proceedings

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MicroRNAs (miRNAs) play pivotal roles in many fundamental aspects of life, such as tissue differentiation, metabolic modulation and cell proliferation control. As miRNA has the characteristics of small size, easy degradation and low abundance, a simple, rapid and specificity method to measure the concentration of miRNA is required. Herein, we present an Electrochemiluminescent Chips system for rapid miRNA detection based on the base stacking hybridization and magnetic beads (MB) enrichment technology. In this design, integrating the microfluidic system with Electrochemiluminescent detection makes it easy to assemble the multiple assay steps (e.g., sample preparation, incubation, washing and detection) and let the device convenient to carry. This method is very fast, we can detect miRNA in 19 min with a 10 uL sample volume. Moreover, in this experiment the detection limit was 1 fmol of the pure synthetic miRNA, and the linear range was from 5 fmol to 1 pmol. It also exhibited excellent selectivity over a series of interference miRNA, which display a high level of expression in cancer cell. These results suggest that this method might have potential for cancer diagnosis at the point of care.

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“…One generic microfluidic device has even been designed with both types of detectors: PMT and silicon photodiodes in printed circuit board (PCB) technology [24]. Photodiodes are small devices and are thus easily integrated into a measurement system.…”

Section: Introductionmentioning

confidence: 99%

A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination

Martínez-Olmos¹,

Ballesta-Claver

Palma³

et al. 2009

Sensors

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A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable potentiostat to initialize the chemical reaction and signal processing circuitry, designed to acquire a low level photocurrent with offset cancelation, low pass filtering for noise attenuation and adjustable gain up to 1012 V/A. The one-shot biosensor responds to lactate rapidly, with an acquisition time of 2.5 min, obtaining a linear dependence from 8 × 10−6 to 2 × 10−4 M, a detection limit of 2.4 × 10−6 M and a sensor-to-sensor reproducibility (relative standard deviation, RSD) of around 7–10 % at the medium level of the range.

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PCB Technology-Based Electrochemiluminescence Microfluidic Device for Low-Cost Portable Analytical Systems

Cited by 23 publications

References 20 publications

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Amplification-free detection of miRNA via an ECL chips system

A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination

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