PAX5 alteration-associated gene-expression signatures in B-cell acute lymphoblastic leukemia

Shang, Zhen; Zhao, Yuechao; Zhou, Kuangguo; Xu, Yaxi; Huang, Wei

doi:10.1007/s12185-013-1309-9

Cited by 5 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…But the role of ZNRF1 in biology of cancer has been seldom reported before. Previous research showed that ZNRF1 was associated with leukemia development and downexpression of ZNRF1 might be involved in PAX5 mutation-inducing leukemia (Shang et al 2013). So, ZNRF1 may take part in the regulation of cell proliferation and apoptosis during the development of human cancers.…”

Section: Resultsmentioning

confidence: 99%

ZNRF1 can inhibit proliferation and stemness properties of leukemia NB4 cells

Zhao

et al. 2016

Animal Cells and Systems

Self Cite

View full text Add to dashboard Cite

As a newly discovered E3 ubiquitin ligase, ZNRF1 protein participates in controlling Wallerian degeneration, but the role in cancer biology has been seldom reported. We analyzed the change of proliferation, apoptosis, stemness and protein levels of AKT and STAT5 after leukemia NB4 cells were treated with ZNRF1 siRNA and with expression vector. The results showed that overexpression of ZNRF1 inhibited proliferation and increased apoptosis of NB4 cells, but ZNRF1 siRNA did not influence the proliferation and apoptosis. Over-expression of ZNRF1 diminished cancer stem cell properties and down-regulated AKT and STAT5. This is the first study to show that ZNRF1 inhibited proliferation and stemness properties of leukemia NB4 cells possibly through AKT signal and STAT5 signal. ARTICLE HISTORY

show abstract

Section: Resultsmentioning

confidence: 99%

ZNRF1 can inhibit proliferation and stemness properties of leukemia NB4 cells

Zhao

et al. 2016

Animal Cells and Systems

Self Cite

View full text Add to dashboard Cite

show abstract

“…CBX8 triggers the progression of hepatocellular carcinoma by increasing the expression of hsa-miR-365a-3p down-regulated ZNRF1 (34). Deletion of ZNRF1 may be involved in the mechanism of B-cell acute lymphoblastic leukemia associated with PAX5 alteration (35). This indicates that ZNRF1 is a tumor suppressor gene.…”

Section: Discussionmentioning

confidence: 99%

The Expression and Effection of MicroRNA-499a in High-Tobacco Exposed Head and Neck Squamous Cell Carcinoma: A Bioinformatic Analysis

Gong

Xiang

et al. 2019

Front. Oncol.

View full text Add to dashboard Cite

Background: Few studies have directly investigated the differential expression of microRNAs (miRNAs) in head and neck squamous cell carcinoma (HNSCC) with low, medium, and high tobacco exposure. The purpose of this study is to screen the differentially expressed miRNAs and to investigate their clinical significance and potential biological mechanisms in the three groups of HNSCC. Methods: The datasets of HNSCC were obtained from The Cancer Genome Atlas (TCGA). The edgeR package was used to determine differentially expressed miRNAs and genes among the three groups of HNSCC. Statistical methods were applied to assess the clinical significance of miRNA and its correlation with genes. The correlation between gene expression and clinical characteristics was analyzed using weighted gene co-expression network analysis (WGCNA). Three online databases were used to predict the target genes of miRNAs. More importantly, qRT-PCR was employed to verify the differential expression of miRNAs and genes in our patients. Results: 32 differentially expressed miRNAs and 1,820 differentially expressed genes were found among the three groups of HNSCC. Patients with high expression of hsa-miR-499a had lower overall survival than the ones with low expression in high-tobacco exposed HNSCC. Cox regression analysis found that high expression of hsa-miR-499a and female were independent risk factors for prognosis in high-tobacco exposed HNSCC. Chi-square test found that hsa-miR-499a was associated with N stage in high-tobacco exposed HNSCC. WGCNA identified four gene modules associated with N stage in high-tobacco exposed HNSCC. Then three online databases were used to predict potential target genes for hsa-miR-499a, which were AEBP2 and ZNRF1. Pearson correlation analysis showed that hsa-miR-499a was negatively correlated with AEBP2 and ZNRF1. qRT-PCR supported bioinformatic results that hsa-miR-499a, AEBP2, and ZNRF1 were differentially expressed among the three groups of HNSCC in our patients. Conclusion: 32 differentially expressed miRNAs and 1,820 differentially expressed genes were successfully identified in HNSCC with low, medium, and high tobacco exposure. The patients with high expression of hsa-miR-499a had poor prognoses compared with patients with low expression in high-tobacco exposed HNSCC. Hsa-miR-499a was associated with N stage in high-tobacco exposed HNSCC. AEBP2 and ZNRF1 were the potential target genes of hsa-miR-499a.

show abstract

“…Familiades (2009) 119 yetişkin Pro B-ALL hastasında yaptığı çalışmada, hastaların %30'un da PAX5 mutasyonu tespit edilmiştir (63). Shang (2013) pediatrik ve yetişkin B-ALL hastalarında, PAX-5 gen değişimleri taşıyan bireylerde, ZNRF1 ifadelenmesinin önemli ölçüde azaldığını rapor etmişlerdir ve PAX5 değişimleri taşıyan hastalarda ZNRF1'in lösemi gelişimine neden olabileceği görüşünü bildirmişlerdir (64). Shah (2013) öncü B-ALL örneklerinde 2 farklı aileyi oluşturan bireylerde, eksom sekanslama tekniği ile PAX5 geninde tekrarlayan germline bir mutasyon (p.Gly183Ser) tanımlamışlardır, bu mutasyon genin octapeptid bölgesinde olup, bu mutasyonun öncü B-ALL patogenezinde önemli olduğu rapor etmişlerdir.…”

Section: Pax5: Transkripsiyon Faktörü Olanunclassified

“…Bhücrelerinden plazma hücresi oluşum aşamasına kadar görev ifadelenmesi devam eder. Bu nedenle PAX5'in B hücre gen ifadelenmesinde anahtar gen olduğu düşünülmektedir(56). PAX5, 9. kromozomun kısa kolu 9.p13 te lokalize olmuştur, 10 ekzondan oluşmaktadır (13, 57).…”

unclassified

Molecular Approach to Pediatric Precursor B-ALL

Balı¹

2019

Zeynep Kamil Tıp Bülteni

View full text Add to dashboard Cite

Lösemi, çocukluk çağında en sık görülen malign hastalıktır. Bu hastalık yaklaşık 150 yıl önce tanımlanmıştır, ancak son 30 yıllık süreçte tedavide %90'lara varan bir başarı oranına ulaşılabilmiştir. Bu başarılı sonuçlara ulaşılmasında çoklu ilaç uygulamaları, santral sinir sistemi profilaksisi, idame ve destek tedavi uygulamaları etkili olmuştur. Tedavide bu kadar başarılı sonuçların alınmasına rağmen nüks lösemi için bir risk olmaya devam etmekte ve ALL hastalarının %20'sinde görülmektedir. Tedaviden alınan farklı sonuçlar diğer bütün kanser tiplerinde olduğu gibi lösemi'nin de heterojen bir yapıya sahip olduğunu işaret etmektedir. Bu nedenle erken, doğru bir teşhis ile daha etkin bir tedavinin ancak kişiye özgü (hastalık alt gruplarına) tedavi, yöntem ve müdahale stratejilerinin geliştirilmesi ile mümkün olabileceği öngörülmektedir. Bu kapsamda diğer bütün kanser tiplerinde olduğu gibi "lösemi genomunda" yapısal ve/veya işlevsel bozukluk gösteren genler, lösemi tanısı, tedavisi ve nüksünün önlenebilmesi için yeni prognostik araçlar olabilme potansiyeli taşımaktadır.

show abstract

PAX5 alteration-associated gene-expression signatures in B-cell acute lymphoblastic leukemia

Cited by 5 publications

References 20 publications

ZNRF1 can inhibit proliferation and stemness properties of leukemia NB4 cells

ZNRF1 can inhibit proliferation and stemness properties of leukemia NB4 cells

The Expression and Effection of MicroRNA-499a in High-Tobacco Exposed Head and Neck Squamous Cell Carcinoma: A Bioinformatic Analysis

Molecular Approach to Pediatric Precursor B-ALL

Contact Info

Product

Resources

About