Patterns of Protein Accumulation in Developing Anthers of Lilium longiflorum Correlate with Histological Events

The loculus content and tapetum during pollen development in Lilium

“…In accordance with earlier works, we showed that from the microspore mother cell stage to the young microspore stage, the tapetum is characterized by cell polarization (Murgia et al 1991), RER abundance (Steer 1977), multiplication of dictyosomes and associated polysaccharide-containing vesicles (Pacini and Juniper 1979;Gori 1982;Pacini and Franchi 1983;Hess and Hesse 1994), and plasma membrane undulations (Pacini and Juniper 1979;Reznickova and Willemse 1980). Further, the fusion of numerous cytoplasmic polysaccharide-containing vesicles with the plasma membrane at the young microspore stage strongly suggests that products of tapetum activity are most probably secreted into the loculus (Wang et al 1992;Balsamo et al l995;Crossley et al 1995). The involvement of mitochondria vesicles in this process was not described earlier and may appear as a fixation artifact.…”

“…Here, the processes of tapetal secretion have been investigated thoroughly. Dictyosomes and endoplasmic reticulum are the most active organelles in the tapetum until the young microspore stage (Reznickova and Willemse 1980), plastids seem to be involved solely in pollenkitt formation (Heslop-Harrison 1968;Dickinson 1973;Reznickova and Willemse 1980;Reznickova and Dickinson 1982), tapetal proteins are probably secreted into the loculus at the time of microsporogenesis (Wang et al 1992;Balsamo et al 1995;Crossley et al l995), and lipid gobules are secreted in the loculus at the dyad stage and give rise to Ubisch bodies (Clément and Audran 1993). However, some traits of tapetum cytology remain unclear in Lilium species (Weber 1992) and none of the research cited investigates loculus content.…”

Overexpression, purification and biochemical characterization of the wound‐induced leucine aminopeptidase of tomato

“…Leaf tissues were harvested and frozen in liquid N 2 at 0, 2 and 4 h after heat treatment. Total proteins were extracted [30].…”

A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development

“…The phenol extraction method was used to extract protein from various vegetative and floral organs of lily plants (Wang et al , 1992). Total protein extracted from anthers of 34–46mm buds was subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and either stained with Coomassie blue or electroblotted onto nitrocellulose (0.45 μm, Millipore Corporation, Billerica MA, USA).…”

“…The supernatant was collected as the exine-released protein fraction. Protein was extracted from each fraction by the phenol extraction method as described previously (Wang et al , 1992). …”