A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development

Abstract

SummaryThe lily anther-specific gene, LLA1271, encodes an adhesin-like protein found for the first time in higher plants. The protein is deposited on the surface of microspores, moderately affecting exine formation.

Stable pH Suppresses Defense Signaling and is the Key to Enhance Agrobacterium-Mediated Transient Expression in Arabidopsis Seedlings

“…The samples were first mixed with fixation solution (2.5% glutaraldehyde, 4% paraformaldehyde in 0.1 M sodium phosphate buffer, pH 7.0) at room temperature overnight. The samples were rinsed with 0.1 M sodium phosphate buffer, pH 7.0 three times and post-fixed in 1% osmium tetroxide (OsO 4 ) for 4 h then rinsed with 0.1 M sodium phosphate buffer, pH 7.0 three times 40 . Before viewing, samples were dehydrated in an ethanol series and dried with use of a Hitachi HCP-2 critical point dryer.…”