Patterns of Phosphate Compounds in Red Blood Cells of Man and Animals

The first case of a complete deficiency of diphosphoglycerate mutase in human erythrocytes.

“…In all probability the observed differences are a result of enzymatic ratios that vary from one species to another. Some other different data can be observed in human erythrocytes that have been stored in ACD solution and that exhibit a fall in their 2,3-DPG concentration (34). FDP and TP were slightly increased in this condition.…”

“…It is of some interest to compare the levels of glycolytic intermediates in the erythrocytes from the propositus with those from some animal species that exhibit a low level of 2,3-DPG in their erythrocytes (34). The concentrations of these compounds in the erythrocytes of these animal species are quite different from those found in the propositus.…”

Studies on the Interaction of Organic Phosphates with Haemoglobin in an Amphibian (Bufo marinus), a Reptile (Trachydosaurus rugosus) and Man

“…To my knowledge B. marinus is the only amphibian in which DPG has been demonstrated to be the primary naturally occurring modifier. Other amphibians, both anurans and urodeles, apparently use ATP primarily, with varying amounts of DPG present (Bartlett 1970;Araki ,et al 1971;Wood 1971). The occurrence of :pPG as a primary modifier of haemoglobin function in the nucleated red blood cells of a species of amphibian indicates that the use of ATP or DPG is not determined only by the presence or absence of nuclei.…”

“…The occurrence of DPG as a primary modifier in B. marinus provides an opportunity for comparison of the red blood cell metabolism of this species with that of the mammals, with a view to discovering if there are any common characteristics which might make it advantageous for the cells of both of these distantly related organisms to use DPG rather thanATP. ATP has previously been demonstrated to be present in reptilian erythrocytes in high concentrations (Rapoport and Guest 1941;Bartlett 1970). However, the effects of stripping and of ATP on the haemoglobin of any reptile had not previously been determined.…”

“…The extract was then neutralized with 10% (w/v) ammonium hydroxide and chromatographed on Dowex 1 x 8, 400 mesh, using a linear gradient of 0-5N ammonium formate, pH 3· 0, as in the method of Bartlett (1970). The E260 nm was determined for each fraction and the fractions were then assayed for total phosphate according to the method of Bartlett (1959).…”