Patterns of Keratinization in Oral Leukoplakia

Shklar, Gerald

doi:10.1001/archotol.1968.00760060402012

Cited by 15 publications

(7 citation statements)

References 8 publications

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Section: Discussionsupporting

confidence: 93%

“…The great variation in the keratinization pattern of oral leukoplakias found in the present study confirms earlier findings by RENSTRUP (1958), SHKLAR (1968) and PRAETORIUS-CLAUSEN (1970). Different suggestions of classifications of oral leukoplakias based on the structure of the surface zone have been presented by, among others, SHKLAR (1968) and PRAETORIUS-CLAUSEN (1970). The occurrence of a multilayered surface zone in the oral mucous membrane with alternating keratotic and parakeratotic bands was described in a histochemical study by TURESKY, GLICK-MAN & PROVOST (1961) and in a microradiographic study by ANNEROTH (1969a).…”

Section: Discussionsupporting

confidence: 93%

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A quantitative microradiographic study of oral leukoplakias

Anneroth

Fornander

Lindström

1976

European J Oral Sciences

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abstract— Biopsies from 13 oral leukoplakias and from normal tissue specimens in the contralateral region of each patient were examined histologically and microradiographically. Microradiographs of freeze‐sectioned and freeze‐dried sections were made and quantitative photometric analyses of the distribution of the dry mass concentration in different epithelial cell layers of both normal and pathologic specimens were performed. In each microradiograph, the thickness of the stratum corneum was determined. The results indicated that in oral leukoplakia two main changes occur in the epithelial surface layer, i.e. an increase of the thickness of the surface epithelial cell layer, and a formation of an epithelial surface zone of cell layers with different degrees of keratinization and varying dry mass concentrations.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

A quantitative microradiographic study of oral leukoplakias

Anneroth

Fornander

Lindström

1976

European J Oral Sciences

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“…15 The formation of keratin bodies and the nuclear anomalies is an adaptive response to cell damage and is only seen in (normally nonkeratinizing) epithelial cells. 16,17 Therefore, it is likely that the overestimation of MN formation with nonspecific stains is only relevant for experiments with epithelial cells, e.g., from the oral cavity, cervix, bladder, and esophagus. This assumption is supported by the findings of Surralles et al, 18 who compared MN frequencies in human lymphocytes stained with DAPI and Giemsa and found even higher frequencies with the former stain.…”

Section: Discussionmentioning

confidence: 99%

Micronucleus to distinguish adenocarcinoma from reactive mesothelial cell in effusion fluid

Kaur

Dey

2009

Diagnostic Cytopathology

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To evaluate the role of the scoring of micronucleated cell (MNC) to distinguish reactive mesothelial cells from adenocarcinoma cells in effusion fluid. A total of 20 cases of unequivocal metastatic adenocarcinoma and 15 controls with reactive mesothelial cell proliferation in ascetic fluid were selected for scoring of the MNC. The numbers of cells having micronuclei were counted per 1000 of the well-preserved cells in May Grunwald Giemsa stained slides in each case. The mean number of MNC in metastatic adenocarcinoma and reactive mesothelial cells were 21 + 6.53 and 2.93 + 2.63, respectively, per 1000 cells. Micronuclei frequency was significantly increased in adenocarcinoma patients compared with controls (Student's t-test, P < 0.001). The scoring of MNC can be used as an additional biomarker and to discriminate between benign reactive mesothelial cells versus metastatic adenocarcinoma in effusion fluids in difficult situation.

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“…Currently, Giemsa stains are also widely used in micronuclei studies with nonepithelial cells originating from a number of organs, e.g., in experiments with polychromatic erythrocytes in bone marrow of rodents (44), lymphocytes (45), splenocytes (46), and liver cells (47), and in in vitro studies with stable cell lines (48). Because the formation of keratin bodies and of the nuclear anomalies described above are an adaptive response to cell damage, which is only seen in (normally nonkeratinizing) epithelial cells (49,50), it is likely that the overestimation of micronuclei formation with nonspecific stains is only relevant for experiments with epithelial cells, e.g., from the oral cavity, cervix, bladder, and esophagus. This assumption is supported by the findings of Surrales et al (51), who compared micronuclei frequencies in human lymphocytes stained with DAPI and Giemsa and found even higher frequencies with the former stain.…”

Section: Discussionmentioning

confidence: 99%

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Nersesyan

Kundi

Atefie

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

167

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Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grü nwald-Giemsa) and DNA-specific (4 ¶,6-diamidino-2-phenylindole, Feulgen, acridine orange) stains, whereas with Giemsa-based stains, the frequencies of micronuclei in smokers were significantly (4-to 5-fold) higher in the smokers group, no significant increase was observed with any of the DNA-specific stains. Furthermore, the evaluation of cells of the two study groups with Feulgen stain showed that oral mucosa cells from smokers had significantly increased levels of nuclear anomalies other than micronuclei.These anomalies are consequences of cell injury found in epithelial cells and are paralleled by formation of keratin bodies in the cytoplasm that resemble micronuclei. Correlation analyses showed that micronuclei frequencies scored in Giemsa-stained slides correlated significantly with karyorrhexis, karyolysis, condensed chromatin, and binucleates, whereas no such correlations were found with DNA-specific stains. These findings indicate that nuclear anomalies (and possibly keratin bodies) may be misinterpreted as micronuclei with nonspecific DNA stains and lead to false-positive results in studies with cells of epithelial origin. Furthermore, our results show that exposure of oral mucosa cells to genotoxic carcinogens contained in tobacco smoke does not lead to induction of micronuclei in these cells. (Cancer Epidemiol Biomarkers Prev 2006;15(10):1835 -40)

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Patterns of Keratinization in Oral Leukoplakia

Cited by 15 publications

References 8 publications

A quantitative microradiographic study of oral leukoplakias

A quantitative microradiographic study of oral leukoplakias

Micronucleus to distinguish adenocarcinoma from reactive mesothelial cell in effusion fluid

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

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