Patterns of interaction between polyribonucleotides and individual DNA strands derived from several vertebrates, bacteria and bacteriophages

Kubinski, H.; Opara-Kubinska, Z.; Szybalski, Waclaw

doi:10.1016/0022-2836(66)90067-2

Cited by 132 publications

(37 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously observed, this complex is partially resistent to ribonuclease treatment performed as usually reported for the detection of DNA-RNA hybrids. The proportion of ribonuclease resistent radioactivity, as determined by membrane filtration (about 14O/,), is consistent with the value deduced by Kubinski et al [6] from the results obtained by density gradient centrifugation. A difference from the results obtained by these authors by ultracentrifugation is that we have not observed a further decrease in DNA bound radioactivity after treatment with ribonulease T 1.…”

Section: Discussionsupporting

confidence: 90%

“…This point deserves further consideration in relation to the fact that both strands of DNA from E. coli have been found to interact with poly G [6].…”

Section: Discussionmentioning

confidence: 99%

“…I n previous works [8,5] it has been demonstrated that only one of the two complementary DNA strands is involved in this kind of interaction with ribosomal RNA, and work subsequently performed using DNA from phage a [9] has shown that only the strand which is physioEnzymes. Pancreatic ribonuclease, ribonucleate pyrimidine-nucleotido-2'-transferase (cyclizing) (EC 2.7.7.16); ribonuclease T 1, ribonucleate guaninenucleotide-2'-transferase (cyclizing) (EC 2.7.7.26); deoxyribonuclease, deoxyribonucleate oligonucleotidohydrolase (EC 3.1.4.5).logically transcribed participates to the formation of pseudohybrids.This kind of DNA-RNA interaction has been observed in DNA-RNA mixtures containing DNA from several bacilli but no formation of a pseudohybrid band had been observed when the mixture contained DNA:from Escherichiu coli [8,5].A similar kind of interaction has been shown to take place between denatured DNA and polyguanylic acid [5,6,10].I n phage 1, in which transcription occurs on alternating regions of both DNA strands [ll, 121 the sites interacting with poly G are located on the transcribed region of each strand [13,14].The interaction a t room temperature between denatured DNA and ribosomal RNA has now been investigated by means of the nitrocellulose membrane filter technique [15] and by gel atration. Moreover we have further investigated DNA-RNA mixtures containing DNA from E. coli and observed in this case too the existence of a DNA-RNA interaction a t room temperature.…”

mentioning

confidence: 82%

“…A similar kind of interaction has been shown to take place between denatured DNA and polyguanylic acid [5,6,10].…”

mentioning

confidence: 85%

“…Biochem., Vol. 6 final concentration of 15O/, (w/v). After 1 h a t 0", the samples were filtered through Millipore filters (HAWP.…”

Section: Preparationmentioning

confidence: 99%

See 4 more Smart Citations

Interaction between Denatured DNA and Ribosomal RNA from Bacteria: Characterisation of DNA Sites Interacting with Ribosomal RNA at Room Temperature

et al. 1968

View full text Add to dashboard Cite

The interaction which takes place a t room temperature between bacterial denatured DNA and ribosomal RNA has been studied by filtration on nitrocellulose filters and on Sephadex columns. The optimal conditions for this kind of interaction have been1determined. The melting curves and the effect of ribonuclease treatment are consistent with the hypothesis that in this kind of hybrids short base paired regions are present and unpaired RNA tails remain which are digested by ribonuclease. The average length of base paired regions has been shown to be about 20 nucleotides.The sites on DNA which can interact with RNA a t room temperature and which are located in all studied cases on the transcribed DNA strand (or transcribed regions of both strands), have been shown by saturation experiments to cover a large proportion (0.55O/, in Bacillus steurothermophilus) of bacterial DNA.An approximate calculation of the number of these sites in DNA from B. stearotermophilus, made on the basis of the present results, indicates the existence of one site per 1800 nucleotide pairs.It is well known that ribosomal RNA can anneal with denatured homologous DNA upon incubation a t 40-60" for several hours and that the sites for ribosomal RNA account for about 0.3O/, of bacterial DNA [I -31. Significant hybridization has been obtained also in heterologous mixtures of ribosomal RNA and DNA from several bacteria; this indicates a high degree of sequence homologies among bacterial cistrons for ribosomal RNA [4]. It has been recently demonstrated [5--81 that a different kind of interaction can take place a t room temperature between denatured DNA from some microorganisms and homologous as well as heterologous ribosomal RNA.This type of DNA-RNA complex, for which the name of pseudohybrid has been proposed [S], is partially resistent to ribonuclease ; its formation was first demonstrated by the appearance of a new band in CsCl density gradients. I n previous works [8,5] it has been demonstrated that only one of the two complementary DNA strands is involved in this kind of interaction with ribosomal RNA, and work subsequently performed using DNA from phage a [9] has shown that only the strand which is physioEnzymes. Pancreatic ribonuclease, ribonucleate pyrimidine-nucleotido-2'-transferase (cyclizing) (EC 2.7.7.16); ribonuclease T 1, ribonucleate guaninenucleotide-2'-transferase (cyclizing) (EC 2.7.7.26); deoxyribonuclease, deoxyribonucleate oligonucleotidohydrolase (EC 3.1.4.5).logically transcribed participates to the formation of pseudohybrids.This kind of DNA-RNA interaction has been observed in DNA-RNA mixtures containing DNA from several bacilli but no formation of a pseudohybrid band had been observed when the mixture contained DNA:from Escherichiu coli [8,5].A similar kind of interaction has been shown to take place between denatured DNA and polyguanylic acid [5,6,10].I n phage 1, in which transcription occurs on alternating regions of both DNA strands [ll, 121 the sites interacting with poly G are located on the transcribed region of eac...

show abstract

Section: Discussionsupporting

confidence: 90%

“…This point deserves further consideration in relation to the fact that both strands of DNA from E. coli have been found to interact with poly G [6].…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 82%

“…A similar kind of interaction has been shown to take place between denatured DNA and polyguanylic acid [5,6,10].…”

mentioning

confidence: 85%

“…Biochem., Vol. 6 final concentration of 15O/, (w/v). After 1 h a t 0", the samples were filtered through Millipore filters (HAWP.…”

Section: Preparationmentioning

confidence: 99%

See 3 more Smart Citations

Interaction between Denatured DNA and Ribosomal RNA from Bacteria: Characterisation of DNA Sites Interacting with Ribosomal RNA at Room Temperature

et al. 1968

View full text Add to dashboard Cite

show abstract

Pattern of Gene Transcription During the Induction of Bacteriophage Lambda Development: A Possible Model for the Control of Gene Expression in a Differentiating System

Gros

Kourilsky

Marcaud

1969

Novartis Foundation Symposia

View full text Add to dashboard Cite

Complexing and denaturation of DNA by methylmereuric hydroxide. II. Ultracentrifugation studies

Gruenwedel

Davidson

1967

Biopolymers

View full text Add to dashboard Cite

When increasing concentrations of methylmercuric hydroxide are added to a Cs2SO4 solution of native DNA, the buoyant density of DNA is unaltered until a critical concentration is reached above which there is a cooperative transition to denatured DNA which now binds so much CH3HgOH that it becomes very dense and nonbuoyant. As increasing concentrations of methylmercuric hydroxide are added to a Cs2So4 solution of denatured DNA, the buoyant density gradually increases, indicating a gradual increase in the amount of methylmercury cation bound. The denatured DNA methylmercury complex becomes nonbuoyant at the same concentration of methylmercuric hydroxide as does the native DNA. These results support our previous interpretation that CH3HgOH reacts with the imino NH bonds of thymine and guanine in nucleic acids. The reaction occurs more or less independently at the different binding sites for denatured DNA, but it occurs cooperatively with simultaneous denaturation for native DNA. The nature of the transition of denatured DNA to the nonbuoyant state is not known, but it is probably due to an abrupt decrease in the degree of hydration of the DNA when its density and hydrophobic character are sufficiently increased by the binding of the methylmercury cation. Direct measurements of the amount of methylmercury bound by DNA, as observed by preparative ultracentrifugation, confirm approximately the buoyant density results as to the amount of methylmercury bound. The possibility of using methylmercuric hydroxide as a reagent for the separation of complementary strands, depending on then thymine of their thymine plus guanine content, is discussed.

show abstract

Patterns of interaction between polyribonucleotides and individual DNA strands derived from several vertebrates, bacteria and bacteriophages

Cited by 132 publications

References 10 publications

Interaction between Denatured DNA and Ribosomal RNA from Bacteria: Characterisation of DNA Sites Interacting with Ribosomal RNA at Room Temperature

Interaction between Denatured DNA and Ribosomal RNA from Bacteria: Characterisation of DNA Sites Interacting with Ribosomal RNA at Room Temperature

Pattern of Gene Transcription During the Induction of Bacteriophage Lambda Development: A Possible Model for the Control of Gene Expression in a Differentiating System

Complexing and denaturation of DNA by methylmereuric hydroxide. II. Ultracentrifugation studies

Contact Info

Product

Resources

About