Patterns of DNA and chromosome variation during in vitro growth in various genotypes of potato

Ramulu, K. Sree; Dijkhuis, P.; Cate, Ch. H. Hänisch Ten; Groot, B. de

doi:10.1016/0168-9452(85)90067-6

Cited by 46 publications

(8 citation statements)

References 31 publications

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“…APM is a phosphoric amide that has a high microtubule depolymerising activity [24]. Prolonged APM treatments can induce micronucleation in plant cells [60]. Propyzamide is a herbicide that induces mitotic arrest [23].…”

Section: Blocking At Mitosismentioning

confidence: 99%

Chemical inhibitors: a tool for plant cell cycle studies

et al. 2000

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Synchrony provides a large number of cells at defined points of the cell cycle. Highly synchronised cells are powerful and effective tools for molecular analyses and for studying the biochemical events of the cell cycle in plants. Usually, plant cell suspensions can be synchronised by chemical agents, which arrest the cell cycle by acting on the driving forces of the cell cycle engine such as cyclin-dependent kinase activity, enzymes involved in DNA synthesis or proteolysis of cell cycle regulators or by acting on the cell cycle apparatus (mitotic spindle). The specificity, reversibility and efficiency of each type of cell cycle inhibitor are described and related to their mode of action. ß

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Section: Blocking At Mitosismentioning

confidence: 99%

Chemical inhibitors: a tool for plant cell cycle studies

et al. 2000

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“…The fused protoplasts were isolated both f r o m leaf mesophyll of the in vitro grown plants and cell suspensions established from plant leaves. As genetic instability in the in vitro cultured Solanum species was frequently reported (Ramulu et al 1985, R a m u l u and Dijkhuis 1986), the present study was undertaken with the aim of examining the nuclear DNA content in the material currently being fused. In addition the influence of two nuclei isolation procedures (mechanical and via protoplasts) on the nuclear DNA content was investigated.…”

Section: Introductionmentioning

confidence: 99%

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

1999

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The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.

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“…Endoreduplication was regarded as the main mechanism of polyploid cell formation (D’Amato 1985). Aneuploid cells may arise from amitosis, chromosome lagging at anaphase, and asymmetrical chromosome distribution (Bhojwani and Razdan 1983; Sree Ramulu et al 1985; Lee and Phillips 1988; Hao et al 2004). The hormones (such as 2,4‐D) supplemented in medium for callus induction and subculture promoted mitotic irregularities and formation of amphiploid and aneuploid cells (Jha and Roy 1982; Li and Zhang 1991).…”

Section: Discussionmentioning

confidence: 99%

Variation of B Chromosome Associated with Tissue Culture in Wheat‐rye Cross

Tian

2009

JIPB

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In vitro variation of B chromosomes was studied by examining the callus cells derived from the immature embryos from a cross of Chinese Spring wheat (Triticum aestivum L.) and Fin 7416 rye (Secale cereale L.) carrying two B chromosomes. In 40-d-old callus cells, the numbers of B chromosomes ranged from one to four in 65.6% of the cells observed. The distribution of B chromosome numbers was associated with the ploidy levels of the normal chromosomes (A chromosomes). The frequency of the cells with high numbers of B chromosomes (i.e., three or four B chromosomes) in the amphiploid cells with 56 A chromosomes was greater than those in the haploid cells with 28 A chromosomes. Although structural changes in the rye A chromosomes were observed, cytological observation and genomic in situ hybridization demonstrated that the rye B chromosomes were conserved in morphological appearance following tissue culture.

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Patterns of DNA and chromosome variation during in vitro growth in various genotypes of potato

Cited by 46 publications

References 31 publications

Chemical inhibitors: a tool for plant cell cycle studies

Chemical inhibitors: a tool for plant cell cycle studies

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

Variation of B Chromosome Associated with Tissue Culture in Wheat‐rye Cross

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