Chemical inhibitors: a tool for plant cell cycle studies

Planchais, Séverine; Glab, Nathalie; Inzé, Dirk; Bergounioux, Catherine

doi:10.1016/s0014-5793(00)01675-6

Cited by 147 publications

(111 citation statements)

References 62 publications

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“…1C). Because the 2-fold increase can be attributed to a doubling of incubation time, only the data for roscovitine represent definitive reversal of inhibition of DNA replication in accordance with previous findings for these inhibitors (29).…”

Section: Involvement Of Dna Replication In Pvalf-and Aba-mediated Actsupporting

confidence: 87%

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S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

Chandrasekharan

Li²,

Bishop

et al. 2003

Journal of Biological Chemistry

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Elucidating the mechanisms by which the transcription machinery accesses promoters in their chromatin environment is a fundamental aspect of understanding gene regulation. The phas promoter is normally constrained by a rotationally and translationally positioned nucleosome over its TATA region except during embryogenesis when it is potentiated by the presence of Phaseolus vulgaris ABI3-like factor (PvALF), a plantspecific transcription factor, and activated by an abscisic acid (

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Section: Involvement Of Dna Replication In Pvalf-and Aba-mediated Actsupporting

confidence: 87%

“…Evidence for Replication and Its Inhibition in Leaves-Histone H4 synthesis occurs during S phase, and H4 transcripts serve as molecular markers for this stage of the cell cycle (29,30). High levels of H4 transcripts were detected in leaves treated with estradiolϩABA (Fig.…”

Section: Dna Replication Arrest Affects the Aba-mediated Activationmentioning

confidence: 96%

S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

Chandrasekharan

Li²,

Bishop

et al. 2003

Journal of Biological Chemistry

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“…EdU incorporation also occurred in cells that were distant from the cut ( Figure 1I, yellow arrows) and that do not divide, suggesting that excision also induces endoreduplication. We also tested the necessity of DNA synthesis for cell cycle progression using aphidicolin, an inhibitor of DNA polymerase a and d that prevents nuclear DNA replication but not endoreduplication (Planchais et al, 2000;Quelo et al, 2002). If leaf cells were arrested in G2-phase, they would be able to divide once after excision in the presence of aphidicolin.…”

Section: Gametophore Leaf Cells Reenter the Cell Cycle In Late S-phasementioning

confidence: 99%

“…Consistently, the dominant-negative CDKA reduced the amount of RM09 and RM55 transcripts ( Figures 6D and 6E). As an alternative to the dominant-negative CDKA, we used the chemical roscovitine, which inhibits CDKs (Planchais et al, 1997(Planchais et al, , 2000. Roscovitine inhibited the accumulation of CYCB;1 (Figure 7A), cytokinesis ( Figure 7B), the accumulation of RM09 ( Figure 7C) and RM55 ( Figure 7D) transcripts, and tip growth ( Figure 7E).…”

Section: Cdka Regulates Protonema-specific Genes and Tip Growthmentioning

confidence: 99%

PhyscomitrellaCyclin-Dependent Kinase A Links Cell Cycle Reactivation to Other Cellular Changes during Reprogramming of Leaf Cells

et al. 2011

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During regeneration, differentiated plant cells can be reprogrammed to produce stem cells, a process that requires coordination of cell cycle reactivation with acquisition of other cellular characteristics. However, the factors that coordinate the two functions during reprogramming have not been determined. Here, we report a link between cell cycle reactivation and the acquisition of new cell-type characteristics through the activity of cyclin-dependent kinase A (CDKA) during reprogramming in the moss Physcomitrella patens. Excised gametophore leaf cells of P. patens are readily reprogrammed, initiate tip growth, and form chloronema apical cells with stem cell characteristics at their first cell division. We found that leaf cells facing the cut undergo CDK activation along with induction of a D-type cyclin, tip growth, and transcriptional activation of protonema-specific genes. A DNA synthesis inhibitor, aphidicolin, inhibited cell cycle progression but prevented neither tip growth nor protonemal gene expression, indicating that cell cycle progression is not required for acquisition of protonema cell-type characteristics. By contrast, treatment with a CDK inhibitor or induction of dominant-negative CDKA;1 protein inhibited not only cell cycle progression but also tip growth and protonemal gene expression. These findings indicate that cell cycle progression is coordinated with other cellular changes by the concomitant regulation through CDKA;1.

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“…It was, however, difficult to monitor the change of CDK activity during the early phase of organogenesis because the dedifferentiation process is initiated in a single differentiated cell within the vascular bundle (28). To overcome this problem, we used a CDK inhibitor, roscovitine, which specifically inhibits CDK activities in animals (23,29) and kinase activities associated with yeast p13 Suc1 in maize (30,31). We confirmed that the CDK2-kinase activity of R2 immunoprecipitates was less sensitive to roscovitine than the CDK activities associated with p13 Suc1 in rice suspension cells (Fig.…”

Section: Up-regulation Of Cdk Activities At the Early Phase Of Culturmentioning

confidence: 99%

Control of in vitro organogenesis by cyclin-dependent kinase activities in plants

Yamaguchi

Kato

Yoshida

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Little is known concerning the mechanisms by which auxin and cytokinin exert their effects on proliferation and differentiation. Cyclin-dependent kinases (CDKs) are major regulators of the eukaryotic cell cycle, thus they are assumed to control cell differentiation as well as proliferation in response to phytohormone signals. Here, we overexpressed rice R2 cDNA, which encodes a CDK-activating kinase, in tobacco leaf explants by using the glucocorticoid-mediated transcriptional induction system. Transient expression of R2 during the first 7 days of culture triggered callus formation in the absence of cytokinin. This phenotype was enhanced by higher expression of R2 or coexpression of cyclin H, and suppressed by treatment with roscovitine, a CDK inhibitor. R2 expression at a later stage did not prevent cells from differentiation into roots, suggesting a restricted period for sensing CDK activities that control differentiation fate of cells during organogenesis.O rganogenesis occurs in various plant tissue cultures in response to exogenously added phytohormones, mainly auxin and cytokinin (1). High auxin͞cytokinin ratios in the medium usually induce root formation whereas low auxin͞ cytokinin ratios promote shoot formation. On the other hand, media containing intermediate auxin͞cytokinin ratios promote disorganized cellular proliferation and callus formation. Previous studies have shown that induction of shoots or roots from explants could be divided into three stages (2-4). In the first stage, the cells acquire competence for subsequent cell proliferation and differentiation; during the second stage, the developmental fate of competent cells is determined; and the third stage is devoted for differentiation and development of determined organs. However, the molecular mechanism(s) that govern the developmental fate of cells during organogenesis, remains poorly understood.For the continuous operation of meristematic organization during plant development, cell division activity must be tightly controlled by machinery that regulates the cell cycle. The major regulators of eukaryotic cell cycle are cyclin-dependent kinases (CDKs) and their regulatory partner cyclins. We previously showed that reduced activity of CDK in planta resulted in differentiation of root initial cells before cessation of cell division (5). This finding suggested that the indeterminate state of initial cells is controlled independent of cell division, and the level of CDK activity might define the differentiation state of cells to coordinate cell division and differentiation in the meristem.Activity of CDKs is also regulated by phosphorylation. CDKactivating kinase (CAK) phosphorylates CDKs at a conserved threonine residue on the T-loop region and activates their enzyme activities. In vertebrate and fission yeast, catalytic subunit of CAK is a member of the CDK family, termed CDK7͞p40 MO15 (6-8), which is activated by making a complex with cyclin H (9-11) and the stabilizing factor MAT1 (12-14). Rice R2 is closely related to CDK7 (15). Previously,...

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Chemical inhibitors: a tool for plant cell cycle studies

Cited by 147 publications

References 62 publications

S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

PhyscomitrellaCyclin-Dependent Kinase A Links Cell Cycle Reactivation to Other Cellular Changes during Reprogramming of Leaf Cells

Control of in vitro organogenesis by cyclin-dependent kinase activities in plants

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