Patterns and kinetics of neurite extension from sympathetic neurons in culture are age dependent

Argiro, Vincent; Johnson, MI

doi:10.1523/jneurosci.02-04-00503.1982

Cited by 63 publications

(26 citation statements)

References 26 publications

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“…Such factors were probably present in our complex medium, which contained embryo extract. Indeed, our estimate of the initial rate of neurite outgrowth, ~40 gm/h, is comparable to rates observed in previous studies of cultured ciliary and sympathetic ganglion neurons (Collins and Dawson, 1982;Bray, 1973;Argiro and Johnson, 1982;Argiro et al, 1984). Unfortunately, nothing is known about factors that might regulate the long term maintenance of neurites in vitro.…”

Section: MVsupporting

confidence: 86%

Changes in the number of chick ciliary ganglion neuron processes with time in cell culture.

Role¹,

Fischbach²

1987

The Journal of Cell Biology

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The purpose of this study was to describe the shape of chick ciliary ganglion neurons dissociated from embryonic day 8 or 9 ganglia and maintained in vitro. Most of the neurons were multipolar during the first three days after plating, with an average of 6.0 processes extending directly from the cell body. The neurons became unipolar with time. The remaining primary process accounted for greater than 90% of the total neuritic arbor. This striking change in morphology was not due to the selective loss of multipolar cells, or to an obvious decline in the health of apparently intact cells. The retraction of processes was neither prevented nor promoted by the presence of embryonic muscle cells. Process pruning occurred to the same extent and over the same time course whether the cells were plated on a monolayer of embryonic myotubes or on a layer of lysed fibroblasts. Process retraction is not an inevitable consequence of our culture conditions. Motoneurons dissociated from embryonic spinal cords remained multipolar over the same period of time. We conclude that ciliary ganglion neurons breed true in dissociated cell culture in that the multipolar-unipolar transition reflects their normal, in vivo, developmental program.

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Section: MVsupporting

confidence: 86%

Changes in the number of chick ciliary ganglion neuron processes with time in cell culture.

Role¹,

Fischbach²

1987

The Journal of Cell Biology

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“…Indeed, in a serum-free culture of aged rat sympathetic ganglion cells [22,23] in which the growth of nonneuronal cells was inhibited and thus neurites were clearly seen, the growth of neurites from aged animals was significantly shorter in comparison with that from newborn and young animals, even in the presence of a full dose of nerve growth factor [23]. A similar reduction in neurite re generation due to aging has also been found in serum-rich culture conditions [1,44],…”

Section: Discussionmentioning

confidence: 81%

Nerve Cells of Adult and Aged Mice Grown in a Monolayer Culture: Age-Associated Changes in Morphological and Physiological Properties of Dorsal Root Ganglion Cells in vitro

Fukuda¹

1985

Dev Neurosci

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In order to differentiate age-associated changes in morphological and physiological properties of mammalian nerve cells, dorsal root ganglion (DRG) cells of aged mice (C57BL/6; 98–99 weeks old) were grown in a monolayer culture. Neurite outgrowth, changes in shape and size of their soma and functional properties of their plasma membranes were compared to those of tissue-cultured DRG cells from young adult mice (4–8 weeks old). Trigeminal root ganglion (TRG) cells of aged mice were also grown in a monolayer culture, and their in vitro growth was compared to that of the aged DRG cells. Nerve cells were dissociated from DRG (or TRG) by digestion with collagenase and by trituration and were grown on collagen-coated plastic dishes for more than 14 days. Growth of neurites and changes in the size and shape of the nerve cell soma were viewed under a phase-contrast microscope, and physiological properties of the plasma membrane were studied by conventional intracellular recordings with a glass microelectrode. Both adult and aged DRG cells grew neurites of various length and underwent changes in shape and size of their soma, which could be divided into 2 stages; early and late. In the early stage of tissue culture (0–60 h in vitro), nerve cells altered their shape from a spherical to a spindle-like form. This change was not associated with the reduction in cell size. In the late stage of the tissue culture (3–14 days and thereafter), the DRG reduced their cell size, while changes in shape remained small. Quantitative comparison of the adult and aged DRG nerve cells revealed the following 3 major differences between the 2 cultures: (1) the survival fraction of the aged DRG cells counted at 36–48 h in vitro was 1/4 to 1/10 of that of the adult DRG cells in 3 different tissue culture trials; (2) reduction in the cell size occurred much earlier in the aged

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“…It has previously been shown that neurite extensions from sympathetic neurons in culture are age dependent [Argiro and Johnson, 1982]. In those investigations the onset time and the initial rate of growth on neurites from DRG from prenatal, perinatal and postnatal rats were determined.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopy Analysis of Neurites Extending from Dorsal Root Ganglia in vitro following Exposure to Intervertebral Disc Cells

Larsson

Brisby²,

Johansson³

et al. 2011

Cells Tissues Organs

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Nucleus pulposus cells from the intervertebral disc have been shown to have inhibiting effects on neurite outgrowth in vitro. The nucleus pulposus consists of at least 2 cell populations, notochordal cells and chondrocyte-like cells. The aim of this study was to analyze the morphology of the neurites, from rat dorsal root ganglia (DRG) in a culture system, after exposure of these 2 cell populations. DRG from perinatal rats was harvested and placed in culture dishes for 24 h. Nucleus pulposus cells from donor rats were separated into 2 populations and applied to the DRG and neurite culture for a further 24 h and compared to control cultures exposed to culture medium without cells. The DRG and neurites were thereafter prepared for scanning or transmission electron microscopy (SEM/TEM). Descriptive SEM and TEM analyses and calculations of the neurite diameter were performed. The visual appearance after SEM and TEM preparation was similar in the three different culture conditions. However, there was a statistically significant reduction of the neurite diameter for the cultures exposed to notochordal cells compared to the cultures exposed to medium and chondrocyte-like cells (TEM preparation). Prominent and frequent pathologic abnormalities in peripheral nerve diseases have been observed with changes in axonal caliber. This study may suggest that a preserved small amount of notochordal cells, as seen in human adults, may play a role in clinical situations where nerve tissue is exposed to disc material, i.e. in disc herniation or degeneration.

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Patterns and kinetics of neurite extension from sympathetic neurons in culture are age dependent

Cited by 63 publications

References 26 publications

Changes in the number of chick ciliary ganglion neuron processes with time in cell culture.

Changes in the number of chick ciliary ganglion neuron processes with time in cell culture.

Nerve Cells of Adult and Aged Mice Grown in a Monolayer Culture: Age-Associated Changes in Morphological and Physiological Properties of Dorsal Root Ganglion Cells in vitro

Electron Microscopy Analysis of Neurites Extending from Dorsal Root Ganglia in vitro following Exposure to Intervertebral Disc Cells

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