Patterned Three‐Dimensional Encapsulation of Embryonic Stem Cells using Dielectrophoresis and Stereolithography

Bajaj, Preeti; Marchwiany, Daniel; Duarte, C. Armando; Bashir, Rashid

doi:10.1002/adhm.201200318

Cited by 48 publications

(34 citation statements)

References 43 publications

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“…Some of the advantages of photolithography systems are their ability to uniformly encapsulate cells throughout the scaffold, their minimal heat production, and their good spatial and temporal control of the reaction kinetics (103). As a result, photolithography has been widely used in tissue engineering to create 3D scaffolds for culturing multiple cell types, such as hepatocytes (104), fibroblasts, C2C12 myoblasts, endothelial cells, cardiac stem cells (56), HT1080 fibrosarcoma cells (105), mouse embryonic stem cells (mESCs) (106), and hippocampal neurons (107). Despite photolithography’s wide use in tissue engineering, it does have some drawbacks.…”

Section: Current Strategies For 3d Biofabricationmentioning

confidence: 99%

3D Biofabrication Strategies for Tissue Engineering and Regenerative Medicine

Bajaj¹,

Schweller

Khademhosseini

et al. 2014

Annu. Rev. Biomed. Eng.

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551

418

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Over the past several decades, there has been an ever-increasing demand for organ transplants. However, there is a severe shortage of donor organs, and as a result of the increasing demand, the gap between supply and demand continues to widen. A potential solution to this problem is to grow or fabricate organs using biomaterial scaffolds and a person’s own cells. Although the realization of this solution has been limited, the development of new biofabrication approaches has made it more realistic. This review provides an overview of natural and synthetic biomaterials that have been used for organ/tissue development. It then discusses past and current biofabrication techniques, with a brief explanation of the state of the art. Finally, the review highlights the need for combining vascularization strategies with current biofabrication techniques. Given the multitude of applications of biofabrication technologies, from organ/tissue development to drug discovery/screening to development of complex in vitro models of human diseases, these manufacturing technologies can have a significant impact on the future of medicine and health care.

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Section: Current Strategies For 3d Biofabricationmentioning

confidence: 99%

3D Biofabrication Strategies for Tissue Engineering and Regenerative Medicine

Bajaj¹,

Schweller

Khademhosseini

et al. 2014

Annu. Rev. Biomed. Eng.

Self Cite

551

418

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“…[59] We have also integrated dielectrophoresis approaches with SL to pattern cells embedded within printed hydrogel structures. [60] Stereolithography thus provides a versatile baseline platform for fabricating complex 3D architectures from cell-laden hydrogels.…”

Section: D Printing Apparatus For Biofabricationmentioning

confidence: 99%

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Raman

Bashir

2017

Adv Healthcare Materials

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how the concept of bioinspiration, or imitating biological design and functionality in synthetic materials, created a new class of "smart" biomimetic materials. Then, we shall discuss how the continuing development of enabling manufacturing technologies, such as 3D printing and microfluidics, has established the separate subdiscipline of biofabrication for tissue engineering, or "building with biology." Finally, we shall investigate the convergence of these two fields into the emerging discipline of biohybrid design, the use of biological materials to power non-natural functional behaviors in synthetic machines. Throughout this report, we will revisit a single class of materials, hydrogels, as a case study of biological design in the context of each subfield, and discuss how the constraints, underlying principles, and end-use applications differ in each case. We will also discuss ethical considerations of biological design, with a special focus on forward engineering non-natural or hypernatural functional behaviors in biohybrid systems. We will conclude with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practices for the next generation of engineers and scientists. Biomimicry and Bioinspired DesignA deeper understanding of the underlying design principles that govern biological systems has inspired the field of biomimicry. [1,2] Observing adaptive phenomena in nature, scientists and engineers have sought to extract the components of biological design responsible for this behavior and replicate the behavior in synthetic materials. Fundamentally, this involves understanding the building blocks or base units from which a biological material is built, the hierarchical assembly of these building blocks, and the interactions and interfaces between them.In this section, we will discuss strategies that engineers and scientists have employed to engineer bioinspired hierarchy, from bottom-up self-assembly and top-down engineered assembly. We will present several key demonstrations of stimulus-responsive hydrogels ranging from the micro-to the macroscale, and investigate novel demonstrations in biomimetic actuation and movement. We will conclude with the remaining challenges in the field of biomimicry and discuss the potential future impact of bioinspired design.The discipline of biological design has a relatively short history, but has undergone very rapid expansion and development over that time. This Progress Report outlines the evolution of this field from biomimicry to biofabrication to biohybrid systems' design, showcasing how each subfield incorporates bioinspired dynamic adaptation into engineered systems. Ethical implications of biological design are discussed, with an emphasis on establishing responsible practices for engineering non-natural or hypernatural functional behaviors in biohybrid systems. This report concludes with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practice...

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“…For example, a 3D stereo-lithographic printer (purchased from Rock Hill, SC, USA) has been developed to fabricate 3D hydrogels for tissue engineering [25,41,42] and biological machines [43]. However, this method of using scanning mirrors to reflect the laser is time-consuming.…”

Section: D Microstructure Arrays Fabricated By Printing Systemmentioning

confidence: 99%

Rapid Fabrication of Hydrogel Microstructures Using UV-Induced Projection Printing

Yang

Liang

et al. 2015

Micromachines

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Fabrication of hydrogel microstructures has attracted considerable attention. A large number of applications, such as fabricating tissue engineering scaffolds, delivering drugs to diseased tissue, and constructing extracellular matrix for studying cell behaviors, have been introduced. In this article, an ultraviolet (UV)-curing method based on a digital micromirror device (DMD) for fabricating poly(ethylene glycol) diacrylate (PEGDA) hydrogel microstructures was presented. By controlling UV projection in real-time using a DMD as digital dynamic mask instead of a physical mask, polymerization of the pre-polymer solution could be controlled to create custom-designed hydrogel microstructures. Arbitrary microstructures could also be fabricated within several seconds (<5 s) using a single-exposure, providing a much higher efficiency than existing methods, while also offering a high degree of flexibility and repeatability. Moreover, different cell chains, which can be used for straightforwardly and effectively studying the cell interaction, were formed by fabricated PEGDA microstructures.

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Patterned Three‐Dimensional Encapsulation of Embryonic Stem Cells using Dielectrophoresis and Stereolithography

Cited by 48 publications

References 43 publications

3D Biofabrication Strategies for Tissue Engineering and Regenerative Medicine

3D Biofabrication Strategies for Tissue Engineering and Regenerative Medicine

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Rapid Fabrication of Hydrogel Microstructures Using UV-Induced Projection Printing

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