Rapid Fabrication of Hydrogel Microstructures Using UV-Induced Projection Printing

Yang, Wenguang; Yu, Haibo; Liang, Wenfeng; Wang, Yuechao; Liu, Lianqing

doi:10.3390/mi6121464

Cited by 54 publications

(35 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this case, the exposure patterns can be changed in real‐time to meet the requirement of high‐throughput fabrication. The operating principle of the DMD‐based fabrication system has been described in detail in our previous work …”

Section: Resultsmentioning

confidence: 99%

“…The optofluidic maskless lithography setup combines DMD‐based maskless lithography with a microfluidic device. The details of the DMD‐based printing system are described in the previous work . In brief, a DMD (Texas Instruments Incorporated, Dallas, Texas, USA) with a 3D computer‐controlled stage (Thorlabs Inc., Newton, New Jersey, USA), projection optics, and a charge‐coupled device camera constituted this fabrication system.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

Yang

et al. 2016

Small

Self Cite

View full text Add to dashboard Cite

3D hydrogel microstructures that encapsulate cells have been used in broad applications in microscale tissue engineering, personalized drug screening, and regenerative medicine. Recent technological advances in microstructure assembly, such as bioprinting, magnetic assembly, microfluidics, and acoustics, have enabled the construction of designed 3D tissue structures with spatially organized cells in vitro. However, a bottleneck exists that still hampers the application of microtissue structures, due to a lack of techniques that combined high-throughput fabrication and flexible assembly. Here, a versatile method for fabricating customized microstructures and reorganizing building blocks composed of functional components into a combined single geometric shape is demonstrated. The arbitrary microstructures are dynamically synthesized in a microfluidic device and then transferred to an optically induced electrokinetics chip for manipulation and assembly. Moreover, building blocks containing different cells can be arranged into a desired geometry with specific shape and size, which can be used for microscale tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

Yang

et al. 2016

Small

Self Cite

View full text Add to dashboard Cite

show abstract

“…Namely, digital micro-mirror device projection printing (DMD-PP) is a stereolithographic method that uses an array of micro-mirrors to spatially modulate a pulsed UV laser, in order to achieve additive crosslinking patterns in a photo-curable material. [17,[39][40][41][42] Similarly, it can also be used for subtractive laser ablation within previously crosslinked layers of the material. An alternative approach is to 3D print a sacrificial mold (a.k.a.…”

Section: Discussionmentioning

confidence: 99%

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

Tong

Pham

Shah

et al. 2019

Preprint

View full text Add to dashboard Cite

According to the U.S. Department of Health & Human Services, nearly 115,000 people in the U.S needed a lifesaving organ transplant in 2018, while only ~10% of them have received it. Yet, almost no artificial FDA-approved products are commercially available todaythree decades after the inception of tissue engineering. It is hypothesized that the major bottlenecks restricting its progress stem from lack of access to the inner pore space of the scaffolds. Specifically, the inability to deliver nutrients to, and clear waste from, the center of the scaffolds limits the size of the products that can be cultured. Likewise, the inability to monitor, and control, the cells after seeding them into the scaffold results in nonviable tissue, with an unacceptable product variability. To resolve these bottlenecks, we present a prototype addressable microfluidics device capable of minimally-invasive fluid and cell manipulation within living cultures. As proof-of-concept, we demonstrate its ability to perform additive manufacturing by seeding cells in spatial patterns (including co-culturing multiple cell types); and subtractive manufacturing, by removing surface adherent cells via targeted trypsin release. Additionally, we show that the device is capable of sampling fluids and performing cell "biopsies" (which can be subsequently sent for ex-situ testing), from any location within its culture chamber. Finally, the on-chip plumbing is completely automated using external electronics. This opens up the possibility to perform long-term computer-driven tissue engineering experiments, where the cell behavior is modulated in response to the minimally-invasive observations (e.g. fluid sampling and cell biopsies) throughout the whole duration of the cultures. It is expected that the proofof-concept technology will eventually be scaled up to 3D addressable microfluidic scaffolds, capable of overcoming the limitations bottlenecking the transition of tissue engineering technologies to the clinical setting.

show abstract

“…The UV light of the SLA printer activates the TPO, resulting in the TPO decomposing to generate two radicals, as previously shown in Figure 2. The free radicals are then available to react with the PEGDA monomer in the photopolymer solution resulting in the opening of the C=C bond present in the PEGDA monomer thus allowing a polymer network to be formed [65]. From conducting FTIR analysis of PEGDA (data not shown) the acrylate groups, C-H bending of the CH 2 =CH-C=O and C-O-C stretching at 811 cm −1 and 1189 cm −1 respectively were identified with similar findings being reported elsewhere [66][67][68].…”

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

Additive Manufacturing of Personalized Pharmaceutical Dosage Forms via Stereolithography

et al. 2019

View full text Add to dashboard Cite

The introduction of three-dimensional printing (3DP) has created exciting possibilities for the fabrication of dosage forms, paving the way for personalized medicine. In this study, oral dosage forms of two drug concentrations, namely 2.50% and 5.00%, were fabricated via stereolithography (SLA) using a novel photopolymerizable resin formulation based on a monomer mixture that, to date, has not been reported in the literature, with paracetamol and aspirin selected as model drugs. In order to produce the dosage forms, the ratio of poly(ethylene glycol) diacrylate (PEGDA) to poly(caprolactone) triol was varied with diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (Irgacure TPO) utilized as the photoinitiator. The fabrication of 28 dosages in one print process was possible and the printed dosage forms were characterized for their drug release properties. It was established that both drugs displayed a sustained release over a 24-h period. The physical properties were also investigated, illustrating that SLA affords accurate printing of dosages with some statistically significant differences observed from the targeted dimensional range, indicating an area for future process improvement. The work presented in this paper demonstrates that SLA has the ability to produce small, individualized batches which may be tailored to meet patients’ specific needs or provide for the localized production of pharmaceutical dosage forms.

show abstract

Rapid Fabrication of Hydrogel Microstructures Using UV-Induced Projection Printing

Cited by 54 publications

References 46 publications

High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

Additive Manufacturing of Personalized Pharmaceutical Dosage Forms via Stereolithography

Contact Info

Product

Resources

About