Allergic asthma is one of the most common phenotypes of asthma, affecting over 330 million people worldwide. The aim of this study is to explore the pathogenesis of allergic asthma by establishing an in vitro model of allergic asthma. Firstly, a microfluidic chip model for studying human epithelial-dendritic cell co-culture has been constructed by combining microfluidics and electrospinning technologies. Then, by utilizing electrospinning technology, a PLGA basement membrane was constructed, and non-contact co-culture of BEAS-2B epithelial cells and dendritic cells on the microfluidic chip was achieved based on this basement membrane. IL-25 and IL-33, as two major pro-inflammatory factors of asthma, were simultaneously detected by combining microfluidic technology and digital ELISA, through the design of optical path. The experimental results showed that the concentrations of IL-25 and IL-33 increased after the addition of dust mite antigen and tended to normalize after the addition of therapeutic drugs. Therefore, the in vitro co-culture microfluidic chip model designed in this study has revealed the pathogenesis of allergic asthma. Moreover, the drug experiments provide important references for future clinical diagnosis and targeted drug therapy.