Pattern recognition analysis of polyamines in the plasma of rat models with adenovirus infection

Polyamine patterns in the cerebrospinal fluid of patients with Parkinson's disease and multiple system atrophy

“…When these normalized values were used as variables to draw star graphs composed of seven rays to visually illustrate PA differences among normal, PD, and MSA, groups were clearly distinct (Fig. 2) compared with numerical data (Table 1), and readily distinguishable from the heptagon shape for the normal group mean, which was previously shown to be an adequate reference pattern [14,15]. Although the normal group mean proved adequate as a control pattern for the patient group, a pressing need exists for large-scale studies of PA change in the CSF of patients with PD and MSA in order to clarify the significance of PA metabolism in these diseases.…”

“…The termini of the lines were joined together to produce the star symbol patterns using the program Microsoft Excel (Microsoft, Redmond, WA), as described elsewhere [14,15].…”

The free fatty acid metabolome in cerebral ischemia following human mesenchymal stem cell transplantation in rats

“…Immunohistochemistry is the localization of antigens or proteins in cells of a tissue section exploiting the principle of antigen-antibody interactions that are visualized by a marker in biological tissues [30][31][32]. Animals were sacrificed 5 days post MCAo.…”

“…FFA in rat plasma samples were determined as their tert-butyldimethylsilyl (TBDMS) derivatives according to our previously described method [27,32]. Briefly, proteins were removed by adding 1 ml of acetonitrile to 0.1 ml of plasma containing 5.0 µg of pentadecanoic acid as an internal standard (IS).…”

“…Rat brain samples were prepared for the FFA assay using our previously described method [27,32]. Total brain tissue placed in 5 ml of distilled water was homogenized at 30,000 rpm for 3 min in an ice-water bath using a rotor/stator-type tissue homogenizer (model Pro 200 Homogenizer, Oxford, UK).…”