The free fatty acid metabolome in cerebral ischemia following human mesenchymal stem cell transplantation in rats

Paik, Man‐Jeong; Li, Wen Yu; Ahn, Young Hwan; Lee, Phil Hyu; Choi, Sangdun; Kim, Kyoung Rae; Kim, Yong Man; Bang, Oh Young; Lee, Gwang

doi:10.1016/j.cca.2008.12.022

Cited by 43 publications

(38 citation statements)

References 33 publications

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“…Paik et al ( 27 ) measured the amount of total FFAs in plasma and brain using a cerebral ischemic rat model. They showed that different FFAs, including palmitic acid (16:0), stearic acid (18:0), and oleic acid (18:1), change.…”

Section: Discussionmentioning

confidence: 99%

Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

Shanta

Choi

Lee

et al. 2012

Journal of Lipid Research

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has been given to cellular phospholipid analyses compared with protein or peptide analyses. However, recent progress in mass spectrometric analysis has enabled the identifi cation of small cellular components including phospholipids and quantifi cation of their cellular expression profi les ( 3, 4 ).ESI MS is widely used for phospholipid analysis. Currently, MALDI imaging MS (MALDI IMS) has become the prime choice because it provides additional advantages over ESI, including simple sample preparation and an abundance of information about molecular structures and spatial data ( 3,(5)(6)(7)(8)(9). The principal advantage of MALDI IMS is its ability to ionize molecules directly from the surface of tissue samples, which can demonstrate the spatial distribution of molecules of interest. However, MALDI IMS must be further improved to resolve technical problems such as the ambiguity of structural determination, unequal ionization effi ciencies of different compounds, and complications due to uneven tissue structure. Glycerophospholipids can be classifi ed according to their head groups, linkage between the polar chain and glycerol backbone, and variability in their FA composition ( 9 ). Depending on the presence of amine or hydroxyl structures on their head group, phospholipids can be ionized in either positive or negative mode. The ionization effi ciency of lipids can also be affected by their head group structure. Occasionally, it is diffi cult to perform MALDI IMS analysis twice in both positive and negative ionization modes on the same tissue sample to identify lipid composition Abstract Neuronal membrane phospholipids are highly affected by oxidative stress caused by ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia to develop an effective approach to prevent brain damage from ischemic injury. The recent development of MALDI imaging MS (MALDI IMS) makes it possible to identify phospholipids that change between damaged and normal regions directly from tissues. In this study, we conducted IMS on rat brains damaged by ischemic injury and detected various phospholipids that showed unique distributions between normal and damaged areas of the brain. Among them, we confi rmed changes in phospholipids such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin by MALDI IMS followed by MS/MS analysis. These lipids were present in high concentrations in the brain and are important for maintenance of cellular structure as well as production of second messengers for cellular signal transduction. Our results emphasize the identifi cation of phospholipid markers for ischemic injury and successfully identifi ed several distinctly located phospholipids in ischemic brain tissue.

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Section: Discussionmentioning

confidence: 99%

Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

Shanta

Choi

Lee

et al. 2012

Journal of Lipid Research

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“…Sample preparation for FFA profiling analysis in the placental tissue samples from normal (n=5) and preeclamptic (n=5) pregnant women was performed according to our previously described method [7][8][9]. Placental tissues were homogenized (3 min, 30,000 rpm) in 5 mL of distilled water in an ice-water bath using a rotor/stator-type tissue homogenizer (Model Pro 200 Homogenizer; Pro Scientific, Monroe, CT).…”

Section: Tert-butyldimethylsilylmentioning

confidence: 99%

“…The same approach was also applied to FFA profiling analysis in the plasma of rats with viral infection, which was useful for the comparative analysis of FFA profiles between two viral infection groups and control [7]. And FFA profiling analysis in plasma and brain was useful for monitoring of altered FFA metabolism following cell therapy with human bone marrow-derived mesenchymal stem cell in ischemia rat model [8].…”

Section: Pattern Recognition Analysismentioning

confidence: 99%

“…The reduction of n-3 PUFAs may be due to to the corresponding mean in the normal group. Subsequently, each normalized value was plotted as a line radiating from a common central point, and the far ends of the 23 lines were joined together to produce a star pattern for each patient using Microsoft Excel® as described previously [6][7][8].…”

Section: Sample Preparation For Assaying Ffas In Placental Tissue Sammentioning

confidence: 99%

See 1 more Smart Citation

Metabolomic Study on Fatty Acids in Placenta of Preeclamptic Pregnancies by Gas Chromatography-Mass Spectrometry

Paik¹,

Shim²,

Seo³

et al. 2017

J Chromatogr Sep Tech

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“…Lastly, stem cell therapy may improve local environmental conditions in ischemic regions. Our pre-clinical studies of brain metabolic profiling have shown that intravenous MSC infusion normalized ischemia-induced changes in free fatty acid levels (44).…”

Section: Enhancing Self-repair Systemsmentioning

confidence: 99%

Current Status of Cell Therapies in Stroke

Bang¹

2009

Int J Stem Cells

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Stroke is a leading cause of death and disability in adults. Recovery after stroke is usually limited as there is no definite therapy to restore lost brain function. Cell therapy is an emerging paradigm in stroke therapy for patients with fixed neurologic deficits. Cell therapy for stroke may be greatly different from cell therapy for other disease conditions; the complexity of central nervous system structures and functions may limit its effectiveness. Recently, there have been several clinical trials of cell therapy for patients with ischemic stroke. In this review, the current status and limitations of cell therapy for stroke will be discussed. In addition, recent efforts and perspectives to improve therapeutic efficacy and safety of cell therapy will be summarized.

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The free fatty acid metabolome in cerebral ischemia following human mesenchymal stem cell transplantation in rats

Cited by 43 publications

References 33 publications

Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

Metabolomic Study on Fatty Acids in Placenta of Preeclamptic Pregnancies by Gas Chromatography-Mass Spectrometry

Current Status of Cell Therapies in Stroke

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