Pattern of Nucleolar Growth in Differentiating Cotton Fibres (Gossypium hirsutum L.)

Peeters, M.‐C.; Voets, S.; Wijsmans, J; Langhe, Edmond De

doi:10.1093/oxfordjournals.aob.a087670

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Thus, the previously reported (Van't Hof, 1998) 25% increase in fiber DNA content early in fiber development does not represent the beginning of successive genome replications. More likely, the reported early increase in DNA content was due to enlarged micronucleoli in these metabolically active cells (Peeters et al, 1988;Van't Hof, 1998).…”

Section: Discussionmentioning

confidence: 99%

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DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers

Taliercio¹,

Hendrix²,

Stewart

2005

American J of Botany

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The cell cycle in cotton (Gossypium hirsutum) fibers is poorly understood. The objective of this study was to evaluate the cell cycle status and DNA content in developing cotton fibers. The DNA content and cell cycle distribution in fiber and hypocotyl cells were determined by flow cytometry. Expression levels of minichrosomal maintenance protein (mcm), cyclin B, and a retinoblastoma-like protein (rb) genes were determined with real-time PCR in fibers and dividing and nondividing tissues. No endoreduplication occurred, nor did genome size or percentage of G1-phase nuclei differ between hypocotyls and fibers. Approximately 13 and 17% of fiber nuclei were in the S phase in 14 days after anthesis (d) fibers and 25 d fibers, respectively. The mcm and cyclin B were expressed at higher levels in fibers than in mature leaves, but expression levels in fibers were less than 15% of meristematic tissues. Rb was expressed in fibers at levels less than 50% of mature leaves or meristematic tissues. Based on an apparent increase in S-phase cells as fibers mature and the low level of expression of genes associated with cell cycle progression, we conclude that S-phase arrest occurs in developing cotton fiber.

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Section: Discussionmentioning

confidence: 99%

“…The S-phase arrest was shown to be independent of fuzz fibers. It is also unlikely that the S-phase arrest phenomenon was an artifact due to nucleoli, because development of these organelles peaks at 6-10 d and declines to a constant value around 20 d (Peeters et al, 1988). In the absence of endoreduplication, [Vol.…”

Section: Discussionmentioning

confidence: 99%

DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers

Taliercio¹,

Hendrix²,

Stewart

2005

American J of Botany

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“…The mean nucleolar volume increased rapidly, reached a maximum before 10 DPA, and was followed by a decline, according to varieties (De Langhe et al , 1978; Peeters et al , 1988). Whether the main contributors to enlargement are RNA, DNA, or protein is unclear.…”

Section: Discussionmentioning

confidence: 99%

Induction of cotton ovule culture fibre branching by co-expression of cotton BTL, cotton SIM, and Arabidopsis STI genes

Wang

Feng

Sun

et al. 2013

Journal of Experimental Botany

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The highly elongated single-celled cotton fibre consists of lint and fuzz, similar to the Arabidopsis trichome. Endoreduplication is an important determinant in Arabidopsis trichome initiation and morphogenesis. Fibre development is also controlled by functional homologues of Arabidopsis trichome patterning genes, although fibre cells do not have a branched shape like trichomes. The identification and characterization of the homologues of 10 key Arabidopsis trichome branching genes in Gossypium arboreum are reported here. Nuclear ploidy of fibres was determined, and gene function in cotton callus and fibre cells was investigated. The results revealed that the nuclear DNA content was constant in fuzz, whereas a limited and reversible change occurred in lint after initiation. Gossypeum arboreum BRANCHLESS TRICHOMES (GaBLT) was not transcribed in fibres. The homologue of STICHEL (STI), which is essential for trichome branching, was a pseudogene in Gossypium. Targeted expression of GaBLT, Arabidopsis STI, and the cytokinesis-repressing GaSIAMESE in G. hirsutum fibre cells cultured in vitro resulted in branching. The findings suggest that the distinctive developmental mechanism of cotton fibres does not depend on endoreduplication. This important component may be a relic function that can be activated in fibre cells.

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Ethylene is a selective ribosomal cistron regulator in Allium cepa epidermal cells

Karagiannis

Pappelis

1993

Mechanisms of Ageing and Development

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Pattern of Nucleolar Growth in Differentiating Cotton Fibres (Gossypium hirsutum L.)

Cited by 3 publications

References 16 publications

DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers

DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers

Induction of cotton ovule culture fibre branching by co-expression of cotton BTL, cotton SIM, and Arabidopsis STI genes

Ethylene is a selective ribosomal cistron regulator in Allium cepa epidermal cells

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