Pattern of immunoglobulin and T-cell receptor-δ/γ gene rearrangements in Iranian children with B-precursor acute lymphoblastic leukemia

Poopak, Behzad; Saki, Najmaldin; Purfatholah, Ali Akbar; Najmabadi, Hossein; Mortazavi, Yousef; Arzanian, Mohammad Taghi; Khosravipour, Gelareh; Haghnejad, Fariba; Salari, Fatemeh; Shahjahani, Mohammad

doi:10.1179/1607845413y.0000000126

Cited by 7 publications

(8 citation statements)

References 38 publications

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“…However, this method has some limitations, including the presence of non-specific gene rearrangements and high diversity of rearrangements in cells (Meleshko et al 2005). Clonal rearrangement of TCR characteristics is a unique assessment and also provides at least the remaining ALL patients with diagnosis of various ALL rearrangements often observed in children with ALL (Poopak 2013). It is important to reach a 0.01 % limit of remaining leukemia cells for monitoring patients, which determines the end of remission induction (Coustan-Smith et al 2000).…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

“…Rearrangement of Ig and TCR genes in different segments (V, D, and J genes) of each gene is a result of somatic mutation. Since each of the leukemic cells is derived from a single clone, after a specific rearrangement, it can be introduced as a sensitive diagnostic marker (Poopak 2013). However, this method has some limitations, including the presence of non-specific gene rearrangements and high diversity of rearrangements in cells (Meleshko et al 2005).…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

“…Factors such as gender, age and white blood cell (WBC) count at diagnosis, immunophenotype, and chromosomal aberrations are significant in predicting disease relapse (Schrappe et al 2000). Detection of molecular-based IgH or TCR gene rearrangements in ALL with a high risk for relapse shows that the MRD of these patients requires protocols and primers enabling rearrangement follow-up (Poopak et al 2015;Scrideli et al 2004). For monitoring of MRD-based patients, they can be divided into three categories: low risk, intermediate risk, and high risk.…”

Section: Tcr Gene Rearrangement For Mrd Monitoringmentioning

confidence: 99%

“…The origin of lymphoid malignancy of B/T cells in the blood, bone marrow (BM), lymph nodes, or thymus may contribute to classification of disease stages and various subgroups of it (Wolvers-Tettero 1991;Campana 2009a;Poopak et al 2015). The prognosis of ALL in children is moderately good, as over 95 % achieve complete remission (CR) and disease-free survival (DFS) rates of 63-83 %.…”

Section: Introductionmentioning

confidence: 99%

“…Similar to normal lymphoid precursor cells, lymphoblastic leukemia cells show numerous rearrangements of Ig/TCR genes, since each of the leukemic cells divide from a clone with the same pattern in the junctional regions of rearranged Ig and TCR genes (DNA fingerprints) so these markers have potential to identify oligoclonality and clonal evolution phenomena (Scrideli et al 2004;Van der Velden et al 2007;van Dongen et al 2015). The ability to detect residual malignant cells from normal cells depends on the specific characteristics of malignant cells, which can be used as markers of clonespecific gene rearrangements for minimal residual disease (MRD) (Deptala and Mayer 2001;Poopak 2013). Therefore, the measurement of residual leukemic cells results in decreased risk of relapse (Kraszewska et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

et al. 2015

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Acute lymphoblastic leukemia is a hematological malignancy of lymphoid progenitor cells associated with excessive proliferation of lymphocytes, and lymphocyte markers can be used for the diagnosis and assessment of disease. Cell surface receptors, including T cell receptor and immunoglobulin (on T and B cells, respectively), are among the most important lymphocyte markers. Gene segment variation, i.e., involvement of several genes in the expression of each receptor, is effective on extensive rearrangement of cell surface receptors. These receptors can be observed in both T-acute lymphoblastic leukemia (ALL) and precursor B-ALL cells. In this study, we have discussed T cell receptor (TCR) gene rearrangements in ALL. As leukemia cell proliferation originates from a unique clone, clone-specific rearrangement can be very helpful in diagnosis and detection of remaining malignant cells from among normal cells for minimal residual disease. On the other hand, each of the TCR genes is associated with translocation in ALL types, and the result of gene rearrangements can be used as prognosis markers.

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Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Section: Tcr Gene Rearrangement For Mrd Monitoringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

et al. 2015

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Mature T- and NK-Cell Neoplasms

Tsang

2021

Practical Oncologic Molecular Pathology

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Minimal residual disease in acute lymphoblastic leukemia: optimal methods and clinical relevance, pitfalls and recent approaches

et al. 2014

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After advances in experimental and clinical testing, minimal residual disease (MRD) assay results are considered a determining factor in treatment of acute lymphoblastic leukemia patients. According to MRD assay results, bone marrow (BM) leukemic burden and the rate of its decline after treatment can be directly evaluated. Detailed knowledge of the leukemic burden in BM can minimize toxicity and treatment complications in patients by tailoring the therapeutic dose based on patients' conditions. In addition, reduction of MRD before allo-HSCT is an important prerequisite for reception of transplant by the patient. In direct examination of MRD by morphological methods (even by a professional hematologist), leukemic cells can be under- or over-estimated due to similarity with hematopoietic precursor cells. As a result, considering the importance of MRD, it is necessary to use other methods including flow cytometry, polymerase chain reaction (PCR) amplification and RQ-PCR to detect MRD. Each of these methods has its own advantages and disadvantages in terms of accuracy and sensitivity. In this review article, different MRD assay methods and their sensitivity, correlation of MRD assay results with clinical symptoms of the patient as well as pitfalls in results of these methods are evaluated. In the final section, recent advances in MRD have been addressed.

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Pattern of immunoglobulin and T-cell receptor-δ/γ gene rearrangements in Iranian children with B-precursor acute lymphoblastic leukemia

Cited by 7 publications

References 38 publications

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

Mature T- and NK-Cell Neoplasms

Minimal residual disease in acute lymphoblastic leukemia: optimal methods and clinical relevance, pitfalls and recent approaches

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