Pattern of expression of adiponectin receptors in human adipose tissue depots and its relation to the metabolic state

Nannipieri, Monica; Bonotti, Alessandra; Anselmino, Marco; Cecchetti, Federica; Madec, Stéphanie; Mancini, Emiliano; Baldi, Simona; Santini, Ferruccio; Pinchera, Aldo; Rossi, María Laura; Ferrannini, Eleuterio

doi:10.1038/sj.ijo.0803676

Cited by 39 publications

(45 citation statements)

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“…In the current study, the mRNA as well as the protein expression of ACRP30 and its two receptors did not differ between VAT and SAT in severely obese patients. Accordingly, it was reported that both ACRP30 receptors are similarly expressed between VAT and SAT (25). However, a decreased ADIPOR1 expression in SAT compared with VAT was also described in obese patients (26).…”

Section: Discussionmentioning

confidence: 99%

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Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Rosa

Monaco

Capasso

et al. 2013

European Journal of Endocrinology

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Objective: Adiponectin is an adipocytokine that exerts beneficial effects on obesity and related disorders by two receptors (ADIPORs). Adiponectin is produced as a monomer that circulates in serum as different oligomers. The oligomerization state and the tissue expression of adiponectin and ADIPORs are linked to its biological activities. In this study, the levels of total adiponectin and its oligomers were evaluated in relation to obesity and surgical weight loss. The expression of adiponectin and ADIPORs was analyzed in visceral and subcutaneous adipose tissues of obese patients. Design and methods: In 25 obese patients and 44 age-and sex-matched controls, the serum levels of adiponectin and its oligomers were measured and compared by ELISA, western blotting, and gel filtration. The expression of adiponectin and ADIPORs in both adipose tissues was evaluated by realtime quantitative PCR and western blotting. Results: The amount of each adiponectin oligomer, including the monomer, increases after weight loss. The reduced circulating levels of adiponectin and its oligomers are not associated with the adipose tissue depot-specific expression of adiponectin and ADIPORs. Conclusions: Our results suggest that in patients, adiposity is associated with the serum concentrations of adiponectin and its oligomers but not with adipose tissue depot-specific expression of adiponectin and ADIPORs. In particular, the increase in adiponectin monomer levels could probably be related to the improvement of the whole-body energy metabolism then being involved in the improvement of adipose tissue function after weight loss. This work indicates the importance of assessing the whole adiponectin oligomeric profile as further potential indicators of adipose tissue functions in obesity.

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Section: Discussionmentioning

confidence: 99%

“…Together with the well-known endocrine role of ACRP30, an autocrine/paracrine role has also been suggested (24); in fact, both ACRP30 receptors have been identified in adipose tissues, but their relative expression in VAT and SAT remains to be defined (25,26).…”

Section: Introductionmentioning

confidence: 99%

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Rosa

Monaco

Capasso

et al. 2013

European Journal of Endocrinology

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“…Interestingly, serum adiponectin levels are inversely correlated with waist circumference and intra-abdominal fat accumulation (48), suggesting that depot differences likely exist in adiponectin secretion. Depot differences in adiponectin gene expression have been reported by some (23,46,49) but not all investigators (18,53,63) and importantly are not always correlated with AT secretion (49) or circulating levels (30). Little is known regarding posttranscriptional regulation of adiponectin.…”

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confidence: 99%

Adiponectin secretion and response to pioglitazone is depot dependent in cultured human adipose tissue

Phillips

Ciaraldi²,

Oh³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

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To test the hypothesis that depot differences exist for adiponectin, fresh and cultured human VAT and SAT from obese type 2 diabetic (T2D) and obese nondiabetic (ND) subjects was examined to determine whether differences in adiponectin content and secretion occurred as a function of depot studied, diabetic status, and response to thiazolidinedione treatment. VAT and SAT were obtained by biopsy and AT explants cultured in defined media for 7 days. Protein expression was assessed by Western blot. Adiponectin content of conditioned medium was determined by radioimmunoassay. Diabetic status had no effect on adiponectin secretion over days 0 -2 of culture. In ND SAT, secretion fell over days 2-4 but was sustained at greater levels vs. T2D SAT. In both ND and T2D VAT, adiponectin secretion was low, similar to T2D SAT. Over the 7-day culture period, cellular adiponectin increased in ND SAT and VAT; it remained unchanged in T2D SAT and VAT. Pioglitazone increased adiponectin secretion and content in all SAT. Pioglitazone failed to increase adiponectin secretion from either ND or T2D VAT and increased cellular content only in ND VAT. AT depot differences exist in the secretion of adiponectin and responsiveness to thiazolidinedione treatment. These data suggest that SAT, not VAT, appears to be the major contributor to increased circulating adipopnectin levels in response to pioglitazone treatment. adipose tissue physiology; type 2 diabetes mellitus; body fat distribution; thiazolidinediones; leptin ADIPOSE TISSUE (AT) is recognized to play an important role in the regulation of lipid/carbohydrate metabolism, energy homeostasis, and insulin sensitivity (55) via release of metabolically active products that regulate insulin sensitivity and fat metabolism (27). Many AT products exert opposing actions on insulin sensitivity, inflammation, and fatty acid metabolism. For example, AT products IL-6, TNF-␣, and resistin may impair insulin sensitivity, increase circulating free fatty acids, and exert proinflammatory effects, whereas others such as adiponectin exert insulin-sensitizing effects, reduce free fatty acids, and dampen inflammation (28). The metabolic impact of a given AT depot is therefore related to the relative profile or balance of mediator release.AT is distributed to a variety of locations, and in humans contributions of SAT and VAT can be distinguished (reviewed in Ref. 59). Individuals with increased visceral (V)AT are at higher risk of developing the metabolic syndrome, type 2 diabetes (T2D), and cardiovascular disease (32, 59) than those with similar amounts of AT as subcutaneous (S)AT (34). Underlying these findings are depot differences in gene and protein expression (36, 37). These findings lend support to the idea that different AT depots are functionally distinct metabolic units.Circulating levels of adiponectin are low in obese and insulin-resistant subjects (62) and are predictive for development of T2D (30). In contrast, adiponectin is high in lean and insulin-sensitive subjects (50). Interestingly, seru...

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“…Similarly, both adiponectin receptors are expressed (mRNA) in human adipose tissue and in isolated adipocytes from both subcutaneous and omental depots. Their levels correlate negatively with indices of obesity and metabolic dysfunction and are increased by physical exercise (Rasmussen et al, 2006, Nannipieri et al, 2007, Blüher et al, 2007. However, expression of the receptors is not proof of locally acting adipokines.…”

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confidence: 99%

The autocrine and paracrine roles of adipokines

Karastergiou

Mohamed‐Ali

2010

Molecular and Cellular Endocrinology

197

128

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Please cite this article as: Karastergiou, K., Mohamed-Ali, V., The autocrine and paracrine roles of adipokines, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 35A c c e p t e d M a n u s c r i p t Abstract.Obesity, defined by an excess of adipose tissue, is often associated with the development of various metabolic diseases. The increased and inappropriate deposition of this tissue contributes to hyperglycemia, hyperlipidemia, insulin resistance, endothelial dysfunction and chronic inflammation. Recent evidence suggests that factors expressed and secreted by the adipose tissue, adipokines, may contribute to the development of these abnormalities by mechanisms including inhibition of adipogenesis, adipocyte hypertrophy and death, immune cell infiltration and disruption of tissue metabolism. The presence of adipokine receptors in adipocytes renders these cells available to autocrine and paracrine effects of adipokines. In this review the reported local effects of adipokines on adipose tissue structure, inflammation and regulation of metabolic functions, in the face of over-nutrition and consequent obesity, are outlined. Elucidating the local regulation of white adipocyte development and function could help in the design of effective, tissue-specific therapies for obesity-associated diseases.

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Pattern of expression of adiponectin receptors in human adipose tissue depots and its relation to the metabolic state

Cited by 39 publications

References 45 publications

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Adiponectin secretion and response to pioglitazone is depot dependent in cultured human adipose tissue

The autocrine and paracrine roles of adipokines

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