1979
DOI: 10.1016/0014-4827(79)90574-3
|View full text |Cite
|
Sign up to set email alerts
|

Pattern of condensation of mouse and Chinese hamster chromosomes in G2 and mitosis of 33258-Hoechst-treated cells

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
14
1

Year Published

1983
1983
2017
2017

Publication Types

Select...
5
1

Relationship

1
5

Authors

Journals

citations
Cited by 36 publications
(16 citation statements)
references
References 28 publications
0
14
1
Order By: Relevance
“…The cells were treated with the fluorochrome 33258-Hoechst during the G 2 phase in order to inhibit the condensation of these normally short c-heterochromatic regions (Marcus et al 1979) and thereby increase the resolving power. As shown Fig.…”
Section: Dnase I Sensitivity Of C-heterochromatin In M Agrestis and mentioning
confidence: 99%
“…The cells were treated with the fluorochrome 33258-Hoechst during the G 2 phase in order to inhibit the condensation of these normally short c-heterochromatic regions (Marcus et al 1979) and thereby increase the resolving power. As shown Fig.…”
Section: Dnase I Sensitivity Of C-heterochromatin In M Agrestis and mentioning
confidence: 99%
“…Additionally, the ability of TOP II inhibitors to alter the rate of cell progression through G2 phase has likewise been demonstrated [11,16,24]. Hoechst 33342 is a fluorochrome that binds to the outside of DNA within the minor groove with preference for A+T rich regions of the genome [10,19,33] and it inhibits Top II-mediated DNA relaxation and decatenation reactions [33] by a process other than stabilization of the cleavable complex [13,33].…”
Section: Accumulation Of Cho Cells In G 2 Phase With Hoechst 33342mentioning
confidence: 99%
“…On the basis of the finding that condensationdecondensation cycles relationed with the phosphorylation and de-acetylation of histones produce changes in chromosomal morphology during the mitotic cycle (Marcus et al, 1979), Goyanes and M6ndez (1981) developed a high resolution Rbanding method by cell treatment with 5-bromodeoxyuridine (5-BrdU) and 33258-Hoechst. 33258-Hoechst is a fluorescent benzimidazol compound which shows a specific binding to A-T rich regions in DNA (Kucherlapati et al, 1975;Goyanes & M6ndez, 1981) and produces a bright fluorescence in these areas (Rocchi et al, 1976); Hayman & Sharp, 1981).…”
Section: Introductionmentioning
confidence: 99%
“…The addition of 33258-Hoechst fluorochrome to a cell culture interferes partially with chromosomal condensation during G 2 phase and early mitosis (prophase and metaphase), and inhibits normal metaphase condensation. This phenomenon produces the appearance of metaphasic chromosomes which are longer than chromosomes isolated from untreated cultures (Marcus et al, 1979). Inspite of this effect, 33258-Hoechst does not interfere with cell division (Marcus et al, 1979).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation