Pattern hair loss: Assessment of microinflammation in miniaturized and terminal hair follicles through horizontal histologic sections

Merlotto, Maira Renata; Ramos, Paulo Müller; Miot, Hélio Amante

doi:10.1016/j.jaad.2020.03.119

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…In line with our results, activated CD4 + T-cell in ltrates around the lower portions of the infundibulum have been demonstrated in specimens from the transitional area of patients with progressive alopecia [38]. Concordantly, female pattern hair loss, a condition usually considered to be the female counter part to male AGA [42], showed histologically more intense CD4 + T cell in ltration around miniaturized follicles in frontal samples [43], suggesting that CD4 + T cells may associate with miniaturization.…”

Section: Discussionsupporting

confidence: 89%

Spatial transcriptome reveals disturbance of peri-infundibular immune infiltration in androgenetic alopecia

Charoensuksira

Tantiwong

Pongklaokam

et al. 2023

Preprint

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Peri-infundibular immune infiltration (PII) is a crucial cluster of immune cells associated with human hair follicles (HFs), with potential importance in HF-immune interactions. Androgenetic alopecia (AGA) is characterized by microinflammation and abnormal immune responses, especially in the PII region. However, the precise patterns of immune dysregulation remain unclear due to limitations in current analysis techniques preserving tissue architecture. Here, we utilized spatial transcriptome profiling, a high-throughput analysis technology, to investigate the immunological disturbances within the PII region. We evaluated overall immune infiltrates, conducted gene set enrichment analysis (GSEA), cellular deconvolution, differential expression analysis, over-representation analysis, protein-protein interaction (PPI) network, and upstream regulator analysis to identify cell types and molecular dysregulation in immune cells. Our results demonstrated significant differences in immune signature between the PII of AGA patients (PII-A) and the PII of control donors (PII-C). PII-A exhibited enrichment in CD4 + helper T cells, distinct immune response patterns, and a bias toward the T helper (Th) 2 response. Immunohistochemistry confirmed the disturbed T cell subpopulations, with increased CD4 + T cells displaying elevated Th2 response but reduced Th1-cytotoxic response compared to PII-C. These findings reveal the unique immune landscapes of PII-A and PII-C, suggesting possibilities for the development of innovative treatment approaches.

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Section: Discussionsupporting

confidence: 89%

Spatial transcriptome reveals disturbance of peri-infundibular immune infiltration in androgenetic alopecia

Charoensuksira

Tantiwong

Pongklaokam

et al. 2023

Preprint

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“…A previous series of 17 women with AGA and 5 normal controls demonstrated a significant association between infiltrate severity and the extent of miniaturization. 4,5 In our study, inflammation was noted adjacent to miniaturized follicles in most cases. Several highly miniaturized follicles were also surrounded by severe inflammation.…”

supporting

confidence: 50%

Perifollicular inflammation and follicular spongiosis in androgenetic alopecia

Plante

Valdebran

Forcucci

et al. 2022

Journal of the American Academy of Dermatology

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“…31 It now needs to be elucidated whether these HF microbiome alterations are functionally relevant, e.g. for the discrete perifollicular inflammation associated with HF miniaturization in androgenetic alopecia 150,151 and for the miniaturization process as such.…”

Section: Androgenetic Alopeciamentioning

confidence: 99%