Patients with Nonalcoholic Fatty Liver Disease (NAFLD) have Higher Oxidative Stress in Comparison to Chronic Viral Hepatitis

Kumar, Amit; Sharma, Arun; Duseja, Ajay; Das, Ashim; Dhiman, Radha K.; Chawla, Yogesh; Kohli, Krishan K.; Bhansali, Anil

doi:10.1016/j.jceh.2012.10.009

Cited by 67 publications

(54 citation statements)

References 34 publications

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“…To our knowledge, this is the first study to compare the level of oxidative stress and anti-oxidative markers among patients with chronic hepatitis C virus infection versus nonalcoholic steatohepatitis. The main finding of our study proved excess level of oxidative and lower anti-oxidant markers in patients with NASH than patients with CHC and healthy volunteers, these results are in line with many previous studies (42,43).…”

Section: Resultssupporting

confidence: 93%

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Oxidative stress biomarkers among Saudi patients with non-alcoholic steatohepatitis versus chronic hepatitis C

Al-Jiffri

2016

Electron J Gen Med

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Objective: As oxidative status may be an influential factor for increasing the progress of the disease and decreasing the effectiveness of the treatment, the aim of this study was to measure the oxidative stress and anti-oxidative markers among Saudi patients with chronic hepatitis C (CHC) versus nonalcoholic steatohepatitis (NASH). Methods: This study was carried out on a sample of 150 consecutive patients (75 with CHC and 75 with NASH), selected from gastroenterology outpatient clinic, King Abdulaziz University Hospital, Jeddah, Saudi Arabia. Also, this study included 75 healthy volunteers, mostly, relatives of the patients who came with them to the clinic. Measurements of oxidative stress and anti-oxidative stress markers were measured for all participants. Results:The mean values of oxidative stress markers included conjugated dienes (CD), malondialdehyde (MDA), nitric oxide (NO) and myeloperoxidase (MPO) were significantly elevated in patients with CHC and patients with NASH when compared with Healthy controls group. Also, these parameters were significantly elevated in patients with NASH than patients with CHC. However, the mean values of anti-oxidative stress markers include glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione (GSH), arylesterase (AE) and paraoxonase (PON) were significantly reduced in patients with CHC and patients with NASH when compared with Healthy controls group. Also, these parameters were significantly reduced in patients with NASH than patients with CHC. Conclusion: In this study, patients with NASH have higher levels of oxidative and lower anti-oxidant markers than patients with CHCs.

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Section: Resultssupporting

confidence: 93%

“…Catalase activity was significantly decreased in both NAFLD and CVH patients in comparison to HVs. Patients with NAFLD had significantly higher SOD activity in comparison to CVH patients (64).…”

Section: Resultsmentioning

confidence: 84%

Oxidative stress biomarkers among Saudi patients with non-alcoholic steatohepatitis versus chronic hepatitis C

Al-Jiffri

2016

Electron J Gen Med

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“…125 When oxidative stress and cytokine levels of NAFLD subjects were compared with those of chronic viral hepatitis and healthy volunteers, NAFLD patients showed significantly higher levels of oxidative stress as well as cytokine levels. 126 Similarly, higher oxidative stress has also been documented in both diabetic and non-diabetic Indian NAFLD patients. 127 Recent studies have established a link between NASH and increased IL-1b production, 128,129 potentially via an increase in inflammasome activation.…”

Section: Cytokinesmentioning

confidence: 96%

The Riddle of Nonalcoholic Fatty Liver Disease: Progression From Nonalcoholic Fatty Liver to Nonalcoholic Steatohepatitis

Sharma

Mitnala

Vishnubhotla

et al. 2015

Journal of Clinical and Experimental Hepatology

128

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Nonalcoholic fatty liver (NAFL) is an emerging global epidemic which progresses to nonalcoholic steatohepatitis (NASH) and cirrhosis in a subset of subjects. Various reviews have focused on the etiology, epidemiology, pathogenesis and treatment of NAFLD. This review highlights specifically the triggers implicated in disease progression from NAFL to NASH. The integrating role of genes, dietary factors, innate immunity, cytokines and gut microbiome have been discussed. ( J CLIN EXP HEPATOL 2015;5:147-158)

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“…IGF1 appears to be one of the most significant miR-379 target genes with regard to promoting the development and progression of NAFLD via the enhancement of cholesterol lipotoxicity. Among other keyword-selected putative target genes, B-cell lymphoma 2 ( BCL2 ), catalase ( CAT ), and cAMP responsive element binding protein 1 ( CREB1 ) are reportedly down-regulated in the liver in NAFLD [30, 97, 98]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [30, 97].…”

Section: Discussionmentioning

confidence: 99%

“…Among other keyword-selected putative target genes, B-cell lymphoma 2 ( BCL2 ), catalase ( CAT ), and cAMP responsive element binding protein 1 ( CREB1 ) are reportedly down-regulated in the liver in NAFLD [30, 97, 98]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [30, 97]. Down-regulation of BCL2 and CAT expression in liver tissue drives hepatocyte apoptosis, which is an important pathologic event in the development and progression of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

Okamoto

Koda

Okamoto

et al. 2019

Preprint

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1 Serum miR-379 expression is related to the development and progression of 2 hypercholesterolemia in non-alcoholic fatty liver disease 3 4 Short title: Serum miR-379 relates hypercholesterolemia in NAFLD 5 6 Abstract28 Introduction: Non-alcoholic fatty liver disease (NAFLD) has a wide spectrum, 29 eventually leading to cirrhosis and hepatic carcinogenesis. We previously reported that 30 a series of microRNAs (miRNAs) mapped in the 14q32.2 maternally imprinted gene 31 region (Dlk1-Dio3 mat) are related to NAFLD development and progression in a mouse 32 model. We examined the suitability of miR-379, a circulating Dlk1-Dio3 mat miRNA, 33 as a human NAFLD biomarker.34 Methods: Eighty NAFLD patients were recruited for this study. miR-379 was selected 35 from the putative Dlk1-Dio3 mat miRNA cluster because it exhibited the greatest 36 expression difference between NAFLD and non-alcoholic steatohepatitis in our 37 preliminary study. Real-time PCR was used to examine the expression levels of 38 miR-379 and miR-16 as an internal control.39 Results: Compared to normal controls, serum miR-379 expression was significantly 40 up-regulated in NAFLD patients. Receiver operating characteristic curve analysis 41 suggested that miR-379 is a suitable marker for discriminating NAFLD patients from 42 controls, with an area under the curve value of 0.72. Serum miR-379 exhibited positive 43 correlations with alkaline phosphatase, total cholesterol, and low-density-lipoprotein 44 cholesterol levels in patients with early stage NAFLD (Brunt fibrosis stage 0 to 1). The 45 correlation between serum miR-379 and cholesterol levels was lost in early stage 46 NAFLD patients treated with statins. Software-based predictions indicated that various 47 energy metabolism-related genes, including insulin-like growth factor-1 (IGF-1) and48 IGF-1 receptor, are potential targets of miR-379.49 Conclusions: Serum miR-379 exhibits high potential as a biomarker for NAFLD.50 miR-379 appears to increase cholesterol lipotoxicity, leading to the development and 4 51 progression of NAFLD, via interference with the expression of target genes, including 52 those related to the IGF-1 signaling pathway. Our results could facilitate future research 53 into the pathogenesis, diagnosis, and treatment of NAFLD. 54 Introduction 55Non-alcoholic fatty liver disease (NAFLD) is an important cause of chronic liver 56 injury, with an increasing incidence worldwide [1]. NAFLD, regarded as a hepatic 57 manifestation of metabolic syndrome, is defined by significant lipid deposition in 58 hepatocytes (excessive numbers of fat-laden hepatocytes are observed by light 59 microscopy), unrelated to excessive alcohol consumption [2]. The prevalence of 60 NAFLD is almost 25% worldwide and expected to increase with increasing incidence of 61 obesity and metabolic diseases such as type 2 diabetes mellitus (T2DM) and 62 hyperlipidemia [3]. 63The mechanism underlying the development of NAFLD has not been fully 64 elucidated. Currently, the multiple parallel hit theory is the most widely ac...

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Patients with Nonalcoholic Fatty Liver Disease (NAFLD) have Higher Oxidative Stress in Comparison to Chronic Viral Hepatitis

Abstract: Differences in the markers of oxidative stress and anti-oxidant status between NAFLD, CVH and healthy volunteers suggest presence of higher oxidative stress in patients with NAFLD.

Cited by 67 publications

References 34 publications

Oxidative stress biomarkers among Saudi patients with non-alcoholic steatohepatitis versus chronic hepatitis C

Oxidative stress biomarkers among Saudi patients with non-alcoholic steatohepatitis versus chronic hepatitis C

The Riddle of Nonalcoholic Fatty Liver Disease: Progression From Nonalcoholic Fatty Liver to Nonalcoholic Steatohepatitis

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

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