Patients- and tissue-specific bio-inks with photoactivated PRP and methacrylated gelatin for the fabrication of osteochondral constructs

Irmak, Gülseren; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.msec.2021.112092

Cited by 14 publications

(17 citation statements)

References 56 publications

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“…Furthermore, interconnected nanopores could be fabricated using 3D printing, with nanopores reported as being able to provide a suitable microenvironment for cell proliferation and attachment [ 11 ]. Multiple types of biomaterials such as hydrogel, ceramic, and metal have since been explored for clinical bone regeneration and repair [ 12 , 13 , 14 ]. Calcium silicate-based ceramic (CS) has demonstrated its expressed biocompatibility and osteoinductivity compared with calcium phosphate bioceramic, which can urge osteogenic differentiation of pre-osteoblasts by promoting the secretion of functional proteins [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

et al. 2021

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Bone defects are commonly found in the elderly and athletic population due to systemic diseases such as osteoporosis and trauma. Bone scaffolds have since been developed to enhance bone regeneration by acting as a biological extracellular scaffold for cells. The main advantage of a bone scaffold lies in its ability to provide various degrees of structural support and growth factors for cellular activities. Therefore, we designed a 3D porous scaffold that can not only provide sufficient mechanical properties but also carry drugs and promote cell viability. Ginsenoside Rb1 (GR) is an extract from panax ginseng, which has been used for bone regeneration and repair since ancient Chinese history. In this study, we fabricated scaffolds using various concentrations of GR with mesoporous calcium silicate/calcium sulfate (MSCS) and investigated the scaffold’s physical and chemical characteristic properties. PrestoBlue, F-actin staining, and ELISA were used to demonstrate the effect of the GR-contained MSCS scaffold on cell proliferation, morphology, and expression of the specific osteogenic-related protein of human dental pulp stem cells (hDPSCs). According to our data, hDPSCs cultivated in GR-contained MSCS scaffold had preferable abilities of proliferation and higher expression of the osteogenic-related protein and could effectively inhibit inflammation. Finally, in vivo performance was assessed using histological results that revealed the GR-contained MSCS scaffolds were able to further achieve more effective hard tissue regeneration than has been the case in the past. Taken together, this study demonstrated that a GR-containing MSCS 3D scaffold could be used as a potential alternative for future bone tissue engineering studies and has good potential for clinical use.

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Section: Introductionmentioning

confidence: 99%

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

et al. 2021

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“…As mentioned above, 3D printing has paved the way towards clinical use by designing high-precision and sophisticated systems, alongside high cost-effectiveness. Since gelatin is able to crosslink in situ as well as to provide biologically suitable properties (ability to promote cell adhesion, proliferation and differentiation), gelatin-based inks or its derivatives (for instance, GelMA) have been extensively exploited in several tissues such as bone, skin and cornea [107][108][109][110].…”

Section: Gelatin As Bioink For 3d Printingmentioning

confidence: 99%

“…The printable hydrogel, in comparison to empty scaffolds, also prolonged degradation time and demonstrated its ability to promote cell adhesion, proliferation and differentiation in vitro as well as to regenerate bone tissue in vivo by attracting endogenous stem cells and creating vascular constructs [108]. Gelatin-derived ink also permits the integration of patient-derived stimulating compounds such as platelet-rich plasma or platelet-rich growth factors [109,111]. The addition of stimulating agents to bioinks may accelerate the regeneration process, since it provokes growths factor release and thus attracts more cells.…”

Section: Gelatin As Bioink For 3d Printingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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“…20% (v/v) PRP-mixed 15% (w/v) methacrylated gelatin (Gel MA) hydrogel showed a slight increase in internal pore size and porosity, higher swelling ratio, and lower compressive modulus compared to those without PRP ( Jiang et al, 2021 ). When PRP composed 50% of the hydrogel with a lower Gel MA concentration and was activated before photo-crosslinking, a significant increase in storage modulus, complex modulus, and weight loss were observed ( Irmak and Gumusderelioglu, 2021 ). It is hard to find a pattern as the quality of platelet derivatives, blending ratio, and cross-linking degree vary from formula to formula and factors interact with each other.…”

Section: Addition Of Platelet Derivatives In Ctementioning

confidence: 99%

“…Constructs are cultured with a mixture of equivalent osteogenic medium and chondrogenic medium in an incubator. AdMSCs were evenly distributed in the osteochondral constructs, differentiated into chondrogenic, hypertrophic, and osteogenic phenotypes based on their location in different phases of the scaffold and formed corresponding tissues in vitro ( Irmak and Gumusderelioglu, 2021 ).…”

Section: Addition Of Platelet Derivatives In Ctementioning

confidence: 99%

Progress of Platelet Derivatives for Cartilage Tissue Engineering

Guo

et al. 2022

Front. Bioeng. Biotechnol.

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Articular cartilage has limited self-regeneration ability for lacking of blood vessels, nerves, and lymph that makes it a great challenge to repair defects of the tissue and restore motor functions of the injured or aging population. Platelet derivatives, such as platelet-rich plasma, have been proved effective, safe, and economical in musculoskeletal diseases for their autologous origin and rich in growth factors. The combination of platelet derivatives with biomaterials provides both mechanical support and localized sustained release of bioactive molecules in cartilage tissue engineering and low-cost efficient approaches of potential treatment. In this review, we first provide an overview of platelet derivatives and their application in clinical and experimental therapies, and then we further discuss the techniques of the addition of platelet derivatives and their influences on scaffold properties. Advances in cartilage tissue engineering with platelet derivatives as signal factors and structural components are also introduced before prospects and concerns in this research field. In short, platelet derivatives have broad application prospects as an economical and effective enhancement for tissue engineering–based articular cartilage repair.

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Patients- and tissue-specific bio-inks with photoactivated PRP and methacrylated gelatin for the fabrication of osteochondral constructs

Cited by 14 publications

References 56 publications

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

Progress in Gelatin as Biomaterial for Tissue Engineering

Progress of Platelet Derivatives for Cartilage Tissue Engineering

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