Pathophysiology of rhinitis. Lactoferrin and lysozyme in nasal secretions.

Raphael, Gordon D.; Jeney, Elizabeth V.M.; Baraniuk, James N.; Kim, I; Meredith, Scott D.; Kaliner, Michael

doi:10.1172/jci114329

Cited by 177 publications

(111 citation statements)

References 27 publications

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“…It is a product of glandular processes in the nasal mucosa. 23 Interestingly, in agreement with the present study, lactotransferrin in serum was recently found to be inversely associated with allergic rhinitis caused by mites. 24 A number of other proteins with antimicrobial activities were detected with significantly changed expression in one or several of the study groups.…”

Section: Discussionsupporting

confidence: 93%

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Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

et al. 2010

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Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.

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Section: Discussionsupporting

confidence: 93%

“…Lactotransferrin is an iron-binding transport protein with antimicrobial properties 23 which probably take part in the host defense. It is a product of glandular processes in the nasal mucosa.…”

Section: Discussionmentioning

confidence: 99%

Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

et al. 2010

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“…The inferior nasal turbinates are rich in submucosal glands, which secrete copious amounts of fluid and mucus to humidify inspired air and trap particles and pathogens. Functional and histological studies have not revealed any significant differences between nasal submucosal glands and those found in lower airways (16,(33)(34)(35)(36)(37), and intact human nasal glands also exhibit CFTR-dependent fluid secretion in response to [cAMP] i -elevating agonists (16,37).…”

Section: Low [Vip] Potentiates Cftr-dependentmentioning

confidence: 97%

cAMP-activated Ca2+ signaling is required for CFTR-mediated serous cell fluid secretion in porcine and human airways

Lee

Foskett²

2010

J. Clin. Invest.

114

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“…The remaining supernatant was frozen at -20 o C until biochemical analysis. Biomarkers analyzed included eosinophil cationic protein (ECP), which is a marker of eosinophil activity (Venge et al, 1987), myeloperoxidase (MPO) from neutrophils in the mucosa (Venge, 1994), lysozyme a marker of neutrophil activity and secretion from parasympathetically innervated mucosal glands (Raphael et al, 1989), and albumin, which is a marker of capillary leakage of plasma proteins (Raphael et al, 1991). Analyses of ECP, MPO and lysozyme were made by radio-immunoassays (RIA) and albumin by nephelometry at the University Hospital of Uppsala.…”

mentioning

confidence: 99%