Pathophysiologische Untersuchungen an einem Verbrennungsmodell der Maus

Abstract

Pathophysiological Studies with a Burn Model in Mice Summary1. A new technique to apply high temperature burns and scalds under controlled conditions in living mice has been established.2. The difference between scalds and high temperature heat injuries with respect to the histological changes, the temperature profile within the skin and the mortality rate has been demonstrated. A mechanism for toxin formation shown to occur in vitro in mammalian skin under standardized energy application was reproduced in vivo.3. The toxic effect of the isolated product and the lethal burn injury (in vivo) upon kidney function measured by serum creatinine and urea has been shown. 4. The relationship between the surface area injured and the animal's body weight and total surface as a critical parameter with respect to the survival chance has been established.

Studies on the structure and biological effects of pyrotoxins purified from burned skin

“…The subcutaneous fat was mechanically removed and the skin pieces were burned for 20 seconds with a copper template heated to 250~ by an electrical resistance. This standard high temperature burn model, which was first described by St~idler et al [6], allows reproducible application of heat to the skin. The burned skin pieces were deep frozen, homogenized in sucrose phosphate buffer, and subsequently centrifuged (2,000 g, 30 rain).…”

Immuntherapeutische Untersuchungen mit Verbrennungstoxin am Modell der Maus

“…Die Serien bestanden aus jeweils 20 Tieren beiderlei Gesehleehtes mit einem Durehsehnittsgewieht yon 25 g. Die Verbrennungen erfolgten in der friiher besehriebenen Weise in einer Gr6ge yon 10 em 2 oder 18% der Oberfli/ehe, was innerhalb yon 5 Tagen bei 90--100°/o der Tiere zum Tode fiihrte [12].…”

Increased Survival Rates by Topical Treatment of Burns with Cerium Nitrate

“…The skin between these incisions was separated bluntly with scissors from the fascia, result ing in a loop 3 X 5 cm. Animals were burned accord ing to a method by Stadtler et al [5] as described elsewhere.…”