Immuntherapeutische Untersuchungen mit Verbrennungstoxin am Modell der Maus

Städtler, K; Allgöwer, M.; Cueni, L. B.; Schoenenberger, G. A.

doi:10.1007/bf01851684

Cited by 9 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…was tolerated by the animals without symptoms. The nontoxic precursor derived froni unburned skin was not toxic to any degree for RES-blocked mice [15,17]. Also, the antitoxie IgG fractions used were found to protect mice from o t h e r w i s e l e t h a l b u r n s in vivo [17].…”

Section: Resultsmentioning

confidence: 96%

“…Specific burn toxins produced in thermally exposed mouse and human skin were shown previously [10,15,17,3] to produce 100~o mortality after i.p. injections of 0.35 mg/g of body weight in RES-blocked mice.…”

Section: Discussionmentioning

confidence: 98%

“…Two weeks after the last immunization, the animals were bled and the serum deeomplemented and stored at --7 0 ° C. The possible therapeutic effects of these sera were checked in mice that received otherwise lethal burn injuries and standard fluid therapy based on a model worked out in our laboratories [18]. These mice were found to have a survival rate of 80--90O/o, compared with a 100~o mortality for the controls, who received no immunotherapy [17].…”

Section: In the Second Set O/experiments Three Groups O] 10 Animmentioning

confidence: 99%

“…Thus, it became possible to mimic a given burn injury experimentally by injecting mouse/human toxin in sublethal doses in animals and to investigate the influence of a pseudomonas infection on mortality. At the same time, the possible protective effects of a resulting antitoxie IgG could be studied while avoiding the stress incurred by producing an actual burn wound [15,17].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Specific impairment of host defense for pseudomonas infection in mice by a burn toxin derived from thermally altered human skin

Müller¹,

Allgöwer²,

Städtler³

et al. 1974

Res. Exp. Med.

Self Cite

View full text Add to dashboard Cite

Summary. The mortality rate for an infection with standardized increasing amounts of a characterized strain of Pseudomonas aeruginosa has been established.In these experiments, 1 × 10 T organisms, causing a reproducible mortality rate of 0--20~o in normal control animals, were used. The mortality of mice due to this standardized pseudomonas infection was significantly increased by preinjection of an otherwise sublethal dose of a specific burn toxin 72 hrs before the organisms were applied in a standard wound pocket. The same effect was demonstrated in mice using a specific burn toxin isolated from human skin. This increased mortality rate was counteracted with a specific hcterologous antitoxic IgG and normalized to the mortality rate of the corresponding controls. A possible pathogenic effect of the isolated toxin, namely generalized damage to the cell wall membranes may enhance the establishment of septicemia and may suggest a way for improving the survival rate of severely burned patients.Key words: Burns --Infection --Burn toxin --Therapy --Antitoxic serum. Zusammen]assung. Die prozentuale Sterbliehkeit von M~usen wurde nach einerInfektion mit steigenden Keimzahlen eines definierten Pseudomonas aeruginosaStammes bestimmt. 1 × l0 T Keime bewirken den Ted yon 0~20°/o der Tiere. Diese Keimzahl wurde als Standard fiir die weiteren Versuche verwendct. Die Sterblichkeitsrate der MSmse nach dieser standardisierten Pseudomonas-Infektion war signifikant erhSht, wenn die Tiere 72 Std vor der Applikation der Keime in eine Wundtasche am Riicken eine einmalige, kleine, an und ftir sich harmlose Gabe eines spezifischen, aus M~Lusehaut isolierten Verbrennungstoxins intraperitoneal erhielten. Die gleiche Wirkung wurde mit einem aus verbrannter menschlicher Haut mittels desselben Isolierungsverfahrens gewonnenen Toxin erreicht. Die Steigerung der Sterbequote wurde durch ein spezifisches, heterologes antitoxisches IgG verhindert,

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: In the Second Set O/experiments Three Groups O] 10 Animmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations