Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India

Katkar, Madhuri; Raghuwanshi, K. S.; Chimote, V. P.; Borkar, S. G.

doi:10.22161/ijeab/1.2.25

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…In our research, we detected a high level of polymorphism and also detected 35 loci overall with an average of 8.7 bands per loci. Our current study was in accordance with the results of Katkar et al (2016) who studied the diversity among the isolates of Xac pv. citri collected from different agro-climate regions of India.…”

Section: Discussionsupporting

confidence: 92%

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Host Dependent and Geographical Structuring of Citrus Canker Bacteria at Peshawar, Pakistan

Ullah¹

2021

IJAB

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Citrus canker disease, caused by Xanthomonas axonopodis pv. Citri (Xac) induces huge losses to citrus, particularly in nurseries. This study assessed the level of genetic diversity and divergence in Xac population prevalent in district Peshawar on various host species, based on isolates collected from different fruit nurseries of Peshawar. Surveillance resulted in collection of 80 samples, out of which 50 bacterial samples were isolated. Genotyping of the isolates using RAPD markers amplified 35 scorable loci with an average of 8.7 loci per primers and level of polymorphism was 100%, i.e., almost all of the isolates genotyped had a different multilocus genotype. The loci GLA-031000 was recorded with the highest gene diversity (0.51) followed by GLB-05_1000 and GLA-03_1100 (0.49). The maximum diversity index (0.50) was recorded for loci GLA-031000, followed by GLB-051000 and GLA-03_1100 (0.48). A high diversity was observed across all locations, with the range of genotypic diversity from 0.778 (detected at Malakandair) to 0.955 (detected at Hayatabad and Tarnab). Divergence between samples collected from different locations was limited as revealed by the PCA, PCoA, phylogenetic tree and network analyses. Samples originated from various hosts also revealed a high diversity across host types with the minimum genotype diversity observed for Eureka Lemon (0.750) and the maximum for sweet lemon (0.966). The divergence for samples collected on various citrus host types was absent. The very small linkage disequilibrium (0.006–0.159) reflected on the potential recombination in the population, which must have direct implications to disease management and resistance exploitation. © 2021 Friends Science Publishers

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Section: Discussionsupporting

confidence: 92%

“…citri (Shehzadi and Naz 2019). Different sets of primers usually designed for specific and target region of bacteria DNA to check all variations among strains of citrus canker (Katkar et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Host Dependent and Geographical Structuring of Citrus Canker Bacteria at Peshawar, Pakistan

Ullah¹

2021

IJAB

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“…While Xcc-1, Xcc-2, Xcc-3, Xcc-4, Xcc-5, Xcc-8, Xcc-9 and Xcc-13 were found to produce very poor in virulent symptoms. The Katkar et al (2016) [16] and Jabeen et al (2011) also confirmed the bacterium in similar manner as performed in this study. 5 indicates the effect of various temperature level on the growth of Xanthomonas citri subsp.…”

Section: B) Pathogenicity Testsupporting

confidence: 88%

“…(2016) [16] . While Jabeen et al (2012) [15] isolated the Xanthomonas by using yeast dextrose calcium carbonate agar medium (YDCA).…”

Section: Results and Discussion A) Disease Samples Collectedmentioning

confidence: 99%

Morphological, biochemical and molecular characterization of Xanthomonas citri subsp. citri isolates from different agroclimatic zones of Maharashtra

Isokar¹,

Bramhankar²,

Das³

et al. 2020

Int. J. Chem. Stud.

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Citrus is one of the important tropical and subtropical fruits in the world as well as in India which belongs to the family Rutaceae and sub-family Aurantioedae. Among all factors responsible for decline in citrus production, citrus canker is one of the most serious problem which is caused by Xanthomonas citri subsp. citri. Xanthomonas citri subsp. citri was successfully isolated on the nutrient agar medium, from the fresh lesions on the leaves of naturally canker infected acid lime leaves collected from different agroclimatic zones of Maharashtra. The fourteen isolates were designated from Xcc-1 to Xcc-14 representing the agroclimatic zone. The studies on physiological characteristics of Xanthomonas citri subsp. citri. Showed the maximum growth of pathogen observe at temperature 30 0 C and at pH level 7. All isolates of bacteria were found negative test for gram's staining while positive for starch hydrolysis, potassium hydroxide test, catalase test, hydrogen sulphide production, gelatin liquefaction, acid and gas, indole production and nitrate reduction. The genetic variability was studied by using five ISSR and RAPD primers. Among ISSR primers Isolate Xcc-11 (Nagpur) had higher value of similarity coefficient (0.85) with Xcc-10 (Wardha), whereas Xcc-12 (Gadchiroli), had lower value of similarity coefficient (0.42) with Xcc-1 (Sindhudurg). The result of RAPD primers reveal that Isolate Xcc-11 (Nagpur) had higher value of similarity coefficient (0.81) with Xcc-10 (Wardha), whereas Xcc-2 (Dapoli), had lower value of similarity coefficient (0.41) with Xcc-13 (Gondia).

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“…Colour variation in the colonies of X. a. pv. citri from pale to dark yellow and variable reaction towards gelatin liquefaction and starch hydrolysis has been reported from the 15 isolates of citrus canker collected 14 agroclimatic zones of India (Katkar et al, 2016). Keeping in view the variability present in pathotype A of the bacterium, present studies were conducted to assess the cultural variability present among different isolates collected from different areas of subtropical zone of Himachal Pradesh.…”

Section: Issn: 2319-7706 Volume 9 Number 6 (2020)mentioning

confidence: 99%

Cultural Variability within Different Isolates of Xanthomonas axonopodis pv. citri Collected from Various Species of Citrus in Different Areas of Subtropical Zone of Himachal Pradesh

Kumar¹,

Jarial²,

Jarial³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India

Abstract: Abstract-In present investigation isolates of

Cited by 9 publications

References 10 publications

Host Dependent and Geographical Structuring of Citrus Canker Bacteria at Peshawar, Pakistan

Host Dependent and Geographical Structuring of Citrus Canker Bacteria at Peshawar, Pakistan

Morphological, biochemical and molecular characterization of Xanthomonas citri subsp. citri isolates from different agroclimatic zones of Maharashtra

Cultural Variability within Different Isolates of Xanthomonas axonopodis pv. citri Collected from Various Species of Citrus in Different Areas of Subtropical Zone of Himachal Pradesh

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