Cytological and molecular investigations were undertaken for parent and progeny derived from a trispecific line [2(Gossypium arboreum 9 G. anomalum) 9 G. hirsutum var. BWR], which was crossed with G. hirsutum var. JLH168. Cytomorphological analysis of the F 1 (G. arboreum 9 G. anomalum), its amphidiploid and progeny from trispecies hybrid showed distorted ploidy segregation with monovalents to hexavalents and high intergenomic (small A 2 and large B 1 ) allosynthetic chromosome pairing. Microsatellite analysis identified three fragments associated with G. arboreum and G. anomalum and six fragments associated with G. hirsutum in derivates of the trispecies line 9 G. hirsutum var. JLH168. Inter-Retrotransposon Amplified Polymorphism (IRAP) analysis revealed fragments of G. arboreum and G. anomalum, only in F 1 and amphidiploid. Chromosomal association and microsatellite analysis of three progeny genotypes (i.e. haploid, hexaploid and tetraploid no. 1) confirmed that they share multigenomic background from the three cotton species (A 2 , A h D h and B 1 genome). The interspecific hybrid cotton genotypes studied are likely to be useful for the introgression of genes from diploid species to commercial upland cultivars.
Thirty-eight Indian potato varieties and fifty-two advanced hybrid lines were analyzed for cytoplasm types using both plastid and mitochondrial genome specific markers. Indian genotypes thus analysed could be broadly grouped into 4 cytoplasm types i.e. T/b (69), W/a (18), W/c (1) and A/e (2). The T/b type cytoplasm, typical of common cultivated potato (ssp. tuberosum) was absent in six released varieties (Kufri Chipsona-1/-2/-3 series, Kufri Jawahar, Kufri Megha and Kufri Himalini) and fifteen out of fifty two hybrids analyzed. This information was further used to predict cytoplasm type on the basis of common shared maternal pedigree in thirty-eight other advanced hybrids, which revealed majority (25) had T/b type cytoplasm with W/a and A/e cytoplasm observed in 12 and 1 genotype, respectively. T/b type cytoplasm was observed in all 28 early bulking hybrids studied along with all old genotypes. It was revealed that considerable broadening of maternal base was observed in recently developed genotypes. W/a type cytoplasm was present in most of processing (all 3 chipping varieties and 9 of 12 MP hybrids) and late blight resistant (11of 23 hybrids) genotypes.
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