Pathological and Immunohistochemical Studies of Choroid Plexus Carcinoma of the Dog

Cantile, Carlo; Campani, Daniela; Menicagli, Michele; Arispici, Mario

doi:10.1053/jcpa.2001.0544

Cited by 33 publications

(52 citation statements)

References 13 publications

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“…The reason for the negative finding for GFAP in canine cases is unknown. In the present case, immunoreactivity for vimentin was slightly positive, which was the same as findings reported in other animals [2,6,10,12]. The immunoreactivity for cytokeratin was also slightly positive in the present case, while was negative in the control dog.…”

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confidence: 92%

“…Immunohistochemistry was performed with labeled streptavidin-biotin (LSAB) method. Rabbit antisera against human glial fibrillary acidic protein (GFAP, prediluted, Dako, Glostrup, Denmark), and mouse monoclonal antibodies against human epidermal keratin (cytokeratin AE1/AE3, 1:20, Dako, Carpinteria, CA) [2] and bovine vimentin (1:20, Chemicon, Temecula, CA) were used as primary antibodies. The reaction products were visualized using the peroxidase and 3,3'-diaminobenzidine system.…”

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confidence: 99%

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Anaplastic Ependymoma in the Cervical Spinal Cord of a Maltese Dog

Michimae

Morita

Sunagawa

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. An 8-year and 6-month-old female Maltese dog showed a stoop with rigidity of her cervix and back. Neurologic examination showed loss of proprioception, and deficiency of pain response. Postmortem examination revealed the neoplastic mass replacing the central area in the cervical spinal cord at the level from 4th to 5th segments. Histologically, the mass was composed of neoplastic ependymal cells. The neoplastic cells showed marked atypism, and occasionally formed ependymal rosettes. Based on the morphologic features, the tumor was diagnosed as anaplastic ependymoma. Immunohistochemistry showed that the neoplastic cells were negative for glial fibrillary acid protein, and slightly positive for vimentin and cytokeratin. KEY WORDS: anaplastic ependymoma, canine, spinal cord.

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confidence: 92%

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confidence: 99%

Anaplastic Ependymoma in the Cervical Spinal Cord of a Maltese Dog

Michimae

Morita

Sunagawa

et al. 2004

The Journal of Veterinary Medical Science

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“…4 In addition to the previously mentioned markers, an earlier study examined epithelial cell adhesion molecule (EpCAM), epithelial membrane antigen, carcinoembryonic antigen, proliferating cell nuclear antigen, S100, vimentin, and MIB-1, none of which showed consistent immunoreactivity against choroid plexus and derived tumors. 1 In human pathology, multiple studies have been performed to identify specific immunomarkers for CPTs to differentiate against metastatic carcinomas of the brain. 2,13 Of the markers that have been explored, Kir7.1 has been demonstrated to be a reliable marker for CPTs.…”

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Kir7.1 immunoreactivity in canine choroid plexus tumors

2016

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Abstract. Choroid plexus neoplasms are uncommon brain tumors in dogs. Choroid plexus carcinomas often spread diffusely throughout the ventricular system and subarachnoid space and, in aggressive forms, can mimic histologic patterns of other carcinomas, including being embedded in a desmoplastic reaction. Although choroid plexus tumors (CPTs) heterogeneously express pan-cytokeratin, little is known about other markers to identify choroid plexus and their associated tumors. Kir7.1, an inward-rectifier potassium channel, is reported to have high diagnostic utility in human neuropathology to distinguish CPTs from other primary brain tumors and cerebral metastases. To determine Kir7.1 expression in the dog brain, we analyzed the immunoreactivity of Kir7.1 in normal brain, gliomas, ependymomas, CPTs, meningiomas, and carcinomas. In normal brain tissue, the immunostaining was restricted to the choroid plexus where there was robust membrane immunoreactivity along the apical border of the cells with less intense cytoplasmic staining. Similar strong immunoreactivity was detected in 12 of 12 CPTs, whereas 5 of 5 gliomas, 4 of 5 ependymomas, 5 of 5 meningiomas, and 5 of 6 carcinomas had no immunoreactivity. One ependymoma and 1 nasal carcinoma with squamous metaplasia were up to 75% immunopositive, with moderate cytoplasmic and membranous immunoreactivity, but lacking the robust apical immunoreactivity pattern. Analysis for immunoreactivity in a tissue microarray failed to yield any other locations in which immunoreactivity was detected. These results, including the distinctive pattern of immunostaining in CPTs, suggest that Kir7.1 is an excellent marker for CPTs in the dog.

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“…Metastasis and invasion along the neuraxis should be used as diagnostic criterion for malignancy of choroid plexus neoplasms (KOESTNER et al, 1999). In dogs, this neoplasm metastatize frequently within the ventricular spaces or the meninges (CANTILE et al, 2002). In the present case, high mitotic index, marked cellular pleomorfism, extensive areas of necrosis and hemorrhage and the occurrence of tumor cells in the pituitary similar to those found in the brain tumor were considered critical in favoring the diagnosis of CPC.…”

Section: Palavras-chave: Doenças De Bovinos Neoplasmas Neuropatologiamentioning

confidence: 65%