1999
DOI: 10.1292/jvms.61.1299
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Pathogenicity of Sendai Viruses Adapted into Polarized MDCK Cells.

Abstract: ABSTRACT. Apically and basally released Sendai viruses (SeV) were obtained after infection of polarized Madin-Darby canine kidney (MDCK) cells grown on permeable membrane culture inserts. After 20 passages of adaptation in MDCK cells, we compared their in vivo and in vitro pathogenicity with the parental Mol-strain of SeV. These viruses had comparable in vitro pathogenicity, but the in vivo pathogenicities were varied. The apically released MDCK-adapted virus showed comparable pathogenicity with the parental v… Show more

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Cited by 2 publications
(2 citation statements)
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“…Infection of mice with WT Sendai virus induces severe respiratory tract pathology and infection with 1,000 WT pfu can lead to weight loss while infection with 1 million pfu is lethal to mice [20]. In vitro , visible signs of cell stress such as chromatin condensation are observed in cells that are infected with WT Sendai virus and most cells die within 72 hours after infection.…”
Section: Resultsmentioning
confidence: 99%
“…Infection of mice with WT Sendai virus induces severe respiratory tract pathology and infection with 1,000 WT pfu can lead to weight loss while infection with 1 million pfu is lethal to mice [20]. In vitro , visible signs of cell stress such as chromatin condensation are observed in cells that are infected with WT Sendai virus and most cells die within 72 hours after infection.…”
Section: Resultsmentioning
confidence: 99%
“…The majority of currently available reporter-expressing viruses feature (e)GFP as the reporter gene, and GFP is also the most widely used reporter for in vivo studies. GFP is relatively small, lacks toxicity, can be continuously synthesized during virus replication and is straightforward to image and quantify without further manipulation (Agungpriyono et al, 1999). Unfortunately, the excitation/emission spectrum of GFP overlaps with hemoglobin, melanin and water (Shcherbakova and Verkhusha, 2013), resulting in poor signal penetration in in vivo situations (Deliolanis et al, 2008).…”
Section: Challenges In the Development And Application Of Reportermentioning
confidence: 99%