Pathogenicity of Sendai Viruses Adapted into Polarized MDCK Cells.

Agungpriyono, Dewi Ratih; Yamaguchi, Ryoji; Tohya, Yukinobu; Uchida, Kazuyuki; Tateyama, Susumu

doi:10.1292/jvms.61.1299

Cited by 2 publications

(2 citation statements)

References 27 publications

(29 reference statements)

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“…Infection of mice with WT Sendai virus induces severe respiratory tract pathology and infection with 1,000 WT pfu can lead to weight loss while infection with 1 million pfu is lethal to mice [20]. In vitro , visible signs of cell stress such as chromatin condensation are observed in cells that are infected with WT Sendai virus and most cells die within 72 hours after infection.…”

Section: Resultsmentioning

confidence: 99%

Induction of Influenza-Specific Mucosal Immunity by an Attenuated Recombinant Sendai Virus

Mironova

Garcin

et al. 2011

PLoS ONE

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BackgroundMany pathogens initiate infection at the mucosal surfaces; therefore, induction of mucosal immune responses is a first level of defense against infection and is the most powerful means of protection. Although intramuscular injection is widely used for vaccination and is effective at inducing circulating antibodies, it is less effective at inducing mucosal antibodies.Methodology/Principal FindingsHere we report a novel recombinant, attenuated Sendai virus vector (GP42-H1) in which the hemagglutinin (HA) gene of influenza A virus was introduced into the Sendai virus genome as an additional gene. Infection of CV-1 cells by GP42-H1 resulted in cell surface expression of the HA protein. Intranasal immunization of mice with 1,000 plaque forming units (pfu) of GP42-H1 induced HA-specific IgG and IgA antibodies in the blood, brochoalveolar lavage fluid, fecal pellet extracts and saliva. The HA-specific antibody titer induced by GP42-H1 closely resembles the titer induced by sublethal infection by live influenza virus; however, in contrast to infection by influenza virus, immunization with GP42-H1 did not result in disease symptoms or the loss of body weight. In mice that were immunized with GP42-H1 and then challenged with 5LD50 (1250 pfu) of influenza virus, no significant weight loss was observed and other visual signs of morbidity were not detected.ConclusionsThese results demonstrate that the GP42-H1 Sendai virus recombinant is able to confer full protection from lethal infection by influenza virus, supporting the conclusion that it is a safe and effective mucosal vaccine vector.

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Section: Resultsmentioning

confidence: 99%

Induction of Influenza-Specific Mucosal Immunity by an Attenuated Recombinant Sendai Virus

Mironova

Garcin

et al. 2011

PLoS ONE

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“…The majority of currently available reporter-expressing viruses feature (e)GFP as the reporter gene, and GFP is also the most widely used reporter for in vivo studies. GFP is relatively small, lacks toxicity, can be continuously synthesized during virus replication and is straightforward to image and quantify without further manipulation (Agungpriyono et al, 1999). Unfortunately, the excitation/emission spectrum of GFP overlaps with hemoglobin, melanin and water (Shcherbakova and Verkhusha, 2013), resulting in poor signal penetration in in vivo situations (Deliolanis et al, 2008).…”

Section: Challenges In the Development And Application Of Reportermentioning

confidence: 99%

Development and application of reporter-expressing mononegaviruses: Current challenges and perspectives

2014

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Reverse genetics allows the generation of recombinant viruses entirely from cDNA. One application of this technology is the creation of reporter-expressing viruses, which greatly increase the detail and ease with which these viruses can be studied. However, there are a number of challenges when working with reporter-expressing viruses. Both the reporter protein itself as well as the genetic manipulations within the viral genome required for expression of this reporter can result in altered biological properties of the recombinant virus, and lead to attenuation in vitro and/or in vivo. Further, instability of reporter expression and purging of the genetic information encoding for the reporter from the viral genome can be an issue. Finally, a practical challenge for in vivo studies lies in the attenuation of light signals when traversing tissues. Novel expression strategies and the continued development of brighter, red and farred shifted reporters and the increased use of bioluminescent reporters for in vivo applications promise to overcome some of these limitations in future. However, a “one size fits all” approach to the design of reporter-expressing viruses has thus far not been possible. Rather, a reporter suited to the intended application must be selected and an appropriate expression strategy and location for the reporter in the viral genome chosen. Still, attenuating effects of the reporter on viral fitness are difficult to predict and have to be carefully assessed with respect to the intended application. Despite these limitations the generation of suitable reporter-expressing viruses will become more common as technology and our understanding of the intricacies of viral gene expression and regulation improves, allowing deeper insight into virus biology both in living cells and in animals.

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Pathogenicity of Sendai Viruses Adapted into Polarized MDCK Cells.

Cited by 2 publications

References 27 publications

Induction of Influenza-Specific Mucosal Immunity by an Attenuated Recombinant Sendai Virus

Induction of Influenza-Specific Mucosal Immunity by an Attenuated Recombinant Sendai Virus

Development and application of reporter-expressing mononegaviruses: Current challenges and perspectives

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