Pathogenicity of Penaeid Rod-shaped DNA Virus(PRDV) to Kuruma Prawn in Different Developmental Stages.

Venegas, Claudia; Nonaka, Lisa; Mushiake, Keiichi; Shimizu, Ken; Nishizawa, Tsutomu; Muroga, Kiyokuni

doi:10.3147/jsfp.34.19

Cited by 31 publications

(20 citation statements)

References 14 publications

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“…It is also possible that the different outcomes may have resulted, at least in part, from different tolerances to WSSV. Indeed, tolerance has already been shown in Macrobrachium rosenbergii (Hameed et al 2000), and WSSV is not pathogenic to the larval and early postlarval stages (<PL6) of Penaeus japonicus (Venegas et al 1999). Like Tsai et al (1999), who successfully cultured a population of P. monodon with a light WSSV infection for more than 13 mo without any outbreak of WSS, results of both the present survey (Table 1) and of Withyachumnarnkul (1999) again suggest that some P. monodon populations may also be tolerant to WSSV at low infection levels.…”

Section: Discussionmentioning

confidence: 95%

Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

Peng¹,

Lo²,

Lin³

et al. 2001

Dis. Aquat. Org.

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In a survey of 27 Penaeus monodon culture ponds stocked with postlarvae (~PL10) at medium density (~40 shrimp m -2 ), single-step nested white spot syndrome virus (WSSV) PCR was used to measure the WSSV infection rates in the shrimp populations within 1 mo after stocking. Seven ponds were initially WSSV-free, and the shrimp in 5 of these were harvested successfully. In the ponds (n = 6) where detection rates were higher than 50%, mass mortality occurred during the growth period, and none of these ponds was harvested successfully. In a subsequent study, P. monodon brooders were classified into 3 groups according to their WSSV infection status before and after spawning: brooders that were WSSV-positive before spawning were assigned to group A; spawners that became WSSV-positive only after spawning were assigned to group B; and group C consisted of brooders that were still WSSV-negative after spawning. WSSV screening showed that 75, 44 and 14%, respectively, of group A, B and C brooders produced nauplii that were WSSV-positive. Most (57%; 16/28) of the brooders in group A produced nauplii in which the WSSV prevalence was high (> 50%).When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only ~20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (~3 × by weight) total harvests. We conclude that testing the nauplii is an effective and practical screening strategy for commercially cultured P. monodon.

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Section: Discussionmentioning

confidence: 95%

Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

Peng¹,

Lo²,

Lin³

et al. 2001

Dis. Aquat. Org.

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“…For immersion challenge, healthy Artemia nauplii ( n = 500 at a density of 125 L −1 ) were reared in sterilized 5‐L beakers containing 4000 mL of sterilized aerated sea water and fed rice bran. The viral inoculum was added to the water at 1 mL L −1 (Venegas, Nonaka, Mushiake, Nishizawa & Muroga 1999; Chen, Lo, Chiu, Chang & Kou 2000). After 3 days, Artemia were reared in sea water free of viral inoculum.…”

Section: Methodsmentioning

confidence: 99%

Experimental vertical transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) from brooders to progeny in Macrobrachium rosenbergii and Artemia

Sudhakaran¹,

Ahmed²,

Haribabu

et al. 2007

Journal of Fish Diseases

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White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. Experiments were carried out to determine the possibility of vertical transmission of M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) in M. rosenbergii and Artemia. Prawn broodstock inoculated with MrNV and XSV by oral or immersion challenge survived without any clinical signs of WTD. The brooders spawned 5-7 days after inoculation and the eggs hatched. The survival rate of larvae gradually decreased, and 100% mortality was observed at the post-larvae (PL) stage. Whitish muscle, the typical sign of WTD, was seen in advanced larval developmental stages. The ovarian tissue and fertilized eggs were found to be positive for MrNV/XSV by reverse transcriptase-polymerase chain reaction (RT-PCR) whereas the larval stages showed positive by RT nested PCR (nRT-PCR). In Artemia, reproductive cysts and nauplii derived from challenged brooders were normal and survival rates were within the expected range for normal rearing conditions. The reproductive cysts were found to be positive for MrNV/XSV by RT-PCR whereas the nauplii showed MrNV/XSV-positive by nRT-PCR. The PL of M. rosenbergii fed nauplii derived from challenged Artemia brooders died at 9 days post-inoculum with clinical signs of WTD.

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“…The beakers were covered to prevent contamination. The inoculum of HPV prepared as described above was introduced to the water only once at a volume equal to 0.1% of the total rearing medium (1 ml/l) (Paynter et al, 1992;Venegas et al, 1999;Chen et al, 2000). The control groups were exposed to healthy PL meat homogenate free from viruses like HPV, MBV, WSSV (tested by PCR).…”

Section: Experimental Design For Artemia Challengementioning

confidence: 99%

Experimental exposure of Artemia to Hepatopancreatic parvo-like Virus and Subsequent transmission to post-larvae of Penaeus monodon

Sivakumar¹,

Sarathi²,

Venkatesan³

et al. 2009

Journal of Invertebrate Pathology

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Pathogenicity of Penaeid Rod-shaped DNA Virus(PRDV) to Kuruma Prawn in Different Developmental Stages.

Cited by 31 publications

References 14 publications

Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

Experimental vertical transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) from brooders to progeny in Macrobrachium rosenbergii and Artemia

Experimental exposure of Artemia to Hepatopancreatic parvo-like Virus and Subsequent transmission to post-larvae of Penaeus monodon

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