Pathogenicity of Campylobacter strains of poultry and human origin from Poland

Wysok, Beata; Wojtacka, Joanna; Kivistö, Rauni

doi:10.1016/j.ijfoodmicro.2020.108830

Cited by 18 publications

(32 citation statements)

References 48 publications

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“…All flaA-SVR alleles assigned to greylag geese and bean geese isolates occurred only once, while among isolates obtained from white-fronted geese, single sequences only occurred twice. This is in contrast with the results obtained in previous studies [30], which reported that the majority of alleles co-existed among poultry (18/28, 64.3%) and human (22/34, 64.7%) isolates. According to Atterby et al [31], strain-specific association to particular bird species is noticeable, and limited contact between wildlife waterfowl species differences in diet or feeding behavior or migration patterns can be seen as the causes of the described situation.…”

Section: Discussioncontrasting

confidence: 99%

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Wildlife Waterfowl as a Source of Pathogenic Campylobacter Strains

2022

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Background: The aim of the study was to determine whether free-living birds belonging to game species whose meat is used for human consumption can constitute a reservoir of pathogenic Campylobacter strains, spreading these bacteria to other hosts or directly contributing to human infection. Methods: A total of 91 cloacal swabs were taken from different species of wildlife waterfowl to estimate the Campylobacter prevalence, the genetic diversity of the isolates, and the presence of virulence genes and to evaluate the antimicrobial resistance. Results: The presence of Campylobacter spp. was confirmed in 32.9% of samples. Based on flaA-SVR sequencing, a total of 19 different alleles among the tested Campylobacter isolates were revealed. The virulence genes involved in adhesion were detected at high frequencies among Campylobacter isolates regardless of the host species. The highest resistance was observed for ciprofloxacin. The resistance rates to erythromycin and tetracycline were observed at the same level. Conclusions: These results suggest that wildlife waterfowl belonging to game species may constitute a reservoir of Campylobacter, spreading these bacteria to other hosts or directly contributing to human disease. The high distribution of virulence-associated genes among wildlife waterfowl Campylobacter isolates make them potentially able to induce infection in humans.

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Section: Discussioncontrasting

confidence: 99%

“…is cytolethal-distending toxin (CDT), encoded by the three adjacent genes cdtA, cdtB, and cdtC [49]. The carriage of cdt complex is common in isolates from poultry [49], swine [45], cattle [50], and human [30,51] isolates. In the present study, it was found that 46.7% of wildlife waterfowl isolates possessed three cdt genes.…”

Section: Discussionmentioning

confidence: 99%

Wildlife Waterfowl as a Source of Pathogenic Campylobacter Strains

2022

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“…It has been reported that C. jejuni cdtB mutants had reduced extra-intestinal invasiveness (Yamasaki et al, 2006) and bowel disturbances (Pokkunuri et al, 2012). A study carried out in Poland showed that Campylobacter strains lacking cdtB and cdtC were noncytotoxic, confirming their roles in toxin production (Wysok et al, 2020). The presence of ggt contributes to the colonization potential of C. jejuni in chicken and mice intestines (Barnes et al, 2007).…”

Section: Introductionmentioning

confidence: 94%

Antimicrobial Resistance Profiles, Virulence Genes, and Genetic Diversity of Thermophilic Campylobacter Species Isolated From a Layer Poultry Farm in Korea

Gahamanyi

Song²,

Yoon³

et al. 2021

Front. Microbiol.

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Thermophilic Campylobacter species are among the major etiologies of bacterial enteritis globally. This study aimed at assessing the antimicrobial resistance (AMR) profiles, virulence genes, and genetic diversity of thermophilic Campylobacter species isolated from a layer poultry farm in South Korea. One hundred fifty-three chicken feces were collected from two layer poultry farms in Gangneung, South Korea. The Campylobacter species were isolated by cultural techniques, while PCR and sequencing were used for species confirmation. Antimicrobial susceptibility testing for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)] was carried out by broth microdilution. Three AMR and nine virulence genes were screened by PCR. Genotyping was performed by flaA-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). Of the 153 samples, Campylobacter spp. were detected in 55 (35.9%), with Campylobacter jejuni and Campylobacter coli being 49 (89.1%) and six (10.9%), respectively. High-level resistance was observed for CIP (100%), NAL (100%), and TET (C. jejuni, 93.9%; C. coli: 83.3%). No resistance was observed for SIT. The missense mutation (C257T) in gyrA gene was confirmed by sequencing, while the tet(O) gene was similar to known sequences in GenBank. The rate of multidrug-resistant (MDR) strains was 8.2%, and they all belonged to C. jejuni. All Campylobacter isolates possessed five virulence genes (cdtB, cstII, flaA, cadF, and dnaJ), but none possessed ggt, while the rates for other genes (csrA, ciaB, and pldA) ranged between 33.3 and 95.9%. The flaA-RFLP yielded 26 flaA types (C. jejuni: 21 and C. coli: five), while the MLST showed 10 sequence types (STs) for C. jejuni and three STs for C. coli, with CC-607 (STs 3611) and CC-460 (ST-460) being predominant. Among the 10 STs of C. jejuni, three were newly assigned. The findings of this study highlight the increased resistance to quinolones and TET, the virulence potential, and the diverse genotypes among Campylobacter strains isolated from the layer poultry farm.

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“…Studies demonstrated that mutant Campylobacter strains were negatively affected in absence of some important genes [13,66]. For instance, Campylobacter strains lacking cdtB and cdtC were not cytotoxic, had reduced colonization, and had extra-intestinal invasiveness [15,85]. Flagellar genes (flaA, flaB, flgB, flgE, and flaC) are involved in various cell functions, like motility and biofilm formation [86,87].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated from Layer Chicken Feces in Gangneung, South Korea by Whole-Genome Sequencing

Gahamanyi

Song²,

Yoon³

et al. 2021

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Thermophilic Campylobacter species of poultry origin have been associated with up to 80% of human campylobacteriosis cases. Layer chickens have received less attention as possible reservoirs of Campylobacter species. Initially, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two archived Campylobacter isolates (Campylobacter jejuni strain 200605 and Campylobacter coli strain 200606) from layer chickens to five antimicrobials (ciprofloxacin, nalidixic acid, erythromycin, tetracycline, and gentamicin) were determined using broth microdilution while the presence of selected antimicrobial resistance genes was performed by polymerase chain reaction (PCR) using specific primers. Whole-genome sequencing (WGS) was performed by the Illumina HiSeq X platform. The analysis involved antimicrobial resistance genes, virulome, multilocus sequence typing (MLST), and phylogeny. Both isolates were phenotypically resistant to ciprofloxacin (MIC: 32 vs. 32 µg/mL), nalidixic acid (MIC: 128 vs. 64 µg/mL), and tetracycline (MIC: 64 vs. 64 µg/mL), but sensitive to erythromycin (MIC: 1 vs. 2 µg/mL) and gentamicin (MIC: 0.25 vs. 1 µg/mL) for C. jejuni strain 200605 and C. coli strain 200606, respectively. WGS confirmed C257T mutation in the gyrA gene and the presence of cmeABC complex conferring resistance to FQs in both strains. Both strains also exhibited tet(O) genes associated with tetracycline resistance. Various virulence genes associated with motility, chemotaxis, and capsule formation were found in both isolates. However, the analysis of virulence genes showed that C. jejuni strain 200605 is more virulent than C. coli strain 200606. The MLST showed that C. jejuni strain 200605 belongs to sequence type ST-5229 while C. coli strain 200606 belongs to ST-5935, and both STs are less common. The phylogenetic analysis clustered C. jejuni strain 200605 along with other strains reported in Korea (CP028933 from chicken and CP014344 from human) while C. coli strain 200606 formed a separate cluster with C. coli (CP007181) from turkey. The WGS confirmed FQ-resistance in both strains and showed potential virulence of both strains. Further studies are recommended to understand the reasons behind the regional distribution (Korea, China, and Vietnam) of such rare STs.

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Pathogenicity of Campylobacter strains of poultry and human origin from Poland

Cited by 18 publications

References 48 publications

Wildlife Waterfowl as a Source of Pathogenic Campylobacter Strains

Wildlife Waterfowl as a Source of Pathogenic Campylobacter Strains

Antimicrobial Resistance Profiles, Virulence Genes, and Genetic Diversity of Thermophilic Campylobacter Species Isolated From a Layer Poultry Farm in Korea

Genomic Characterization of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated from Layer Chicken Feces in Gangneung, South Korea by Whole-Genome Sequencing

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