Pathogenicity of Beauveria bassiana and Metarhizium anisopliae to the tobacco spider mite Tetranychus evansi

Wekesa, Vitalis W.; Maniania, Nguya K.; Knapp, Markus; Boga, Hamadi Iddi

doi:10.1007/s10493-005-0508-3

Cited by 79 publications

(59 citation statements)

References 19 publications

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“…Kanga et al (2002) obtained CL90 for V. destructor of 1.37 x 10 9 conidia mL -1 of an M. anisopliae isolate on day 7 post inoculation, which was higher than that obtained in this trial. For other mite species like Tetranychus evansi, Wekesa et al (2005) point out a lethal concentration between 0.7 x 10 7 and 2.3 x 10 7 conidia mL -1 to achieve the 50% mortality with diverse M. anisopliae isolates, values that are again higher than those found in our trials.…”

Section: Laboratory Trialscontrasting

confidence: 64%

Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

Rodríguez

Gerding

Ceballos

2009

Chilean J. Agric. Res.

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The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 ºC) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations were carried out by spraying and increasing the concentration from zero to 10 8 conidia mL -1 on varroa adults. Lethal concentrations required for 50 and 90% mite mortality (LC50 and LC90) were 3.8 x 10 5 and 8 x 10 7 conidia mL -1, respectively (χ 2 = 2.03). In the autumn field trials, three application methods (doses of 5 x 10 10 conidia per hive) were evaluated. The treatments were: a) conidia stamped on filter paper, located on every second frame inside the hive; b) dry conidia sprinkled on and between frames; and c) dry conidia in a dispenser path at the entrance of the hive. Furthermore, untreated hives were included as controls. After 21 days of treatment, the dry conidia sprinkled on and between frames showed 67% less bees infested by the mite than the control (p < 0.05). Average bee mortality observed was significantly different between treatments (p < 0.05). The sprinkled dry conidia caused the highest mortality of bees. In spring, this treatment produced increased mite mortality and without bee mortality. These results demonstrated that it is possible to develop a biological insecticide based on this isolate to generate an alternative to control V. destructor.

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Section: Laboratory Trialscontrasting

confidence: 64%

Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

Rodríguez

Gerding

Ceballos

2009

Chilean J. Agric. Res.

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“…Fries (=Paecilomyces farinosus), Hirsutella thompsonii Fisher, and Neozygites floridana Weiser and Muma often play an important role in decreasing native populations of phytophagous mites (Chandler et al, 2000;Van der Geest et al, 2000). Searching for microbial control agents in laboratory experiments it has been shown that some of the tested isolates of B. bassiana and M. anisopliae are highly virulent to the active stages of T. urticae (Alves et al, 2002a;Tamai et al, 2002;Chandler et al, 2005) and T. evansi Baker and Pritchard (Wekesa et al, 2005). According to Feng (2006, 2009) some of the tested isolates of B. bassiana, Paecilomyces fumosoroseus (Wize) Brown and Smith and M. anisopliae have high lethal effect on eggs and females of T. urticae.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Draganova

Simova

2010

PESTIC PHYTOMED

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SUMMARYBioassays with five isolates of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuillemin were conducted under laboratory conditions with a goal to estimate their virulence to the two-spotted spider mite Tetranychus urticae Koch. (Acari: Tetranychidae). Common bean plants with mites were treated by spraying conidial suspensions of isolates at concentrations of 10 6 , 10 7 and 10 8 conidia/ml. Lethal effects of the fungal isolates were evaluated as percentages of cumulative daily mortality due to mycoses, corrected for mortality in the control variant. Virulence of the isolates was estimated based on values of the median lethal time (LT 50 ) calculated by probit analysis for the variants treated with conidial suspensions at the concentration of 10 6 conidia/ml.The two-spotted spider mite was found susceptible to the examined isolates of the entomopathogenic fungus B. bassiana.Mycosis caused to T. urticae by the B. bassiana isolates 444 Bb and 445 Bb had fast lethal effect after treatment with conidial suspensions even at the concentration of 10 6 conidia/ ml. The mean mortality values of host individuals were 83.78 ± 3.62% and 68.49 ± 4.28% on the first day, respectively, and up to 100% in both variants on the fourth day.The isolates 426 Bb, 444 Bb and 445 Bb of B. bassiana were highly virulent to two-spotted spider mites with values of the median lethal time varied within overlapped narrow confidence intervals from 0.122 to 1.084 days (average value 0.162 days), from 0.117 to 1.398 days (average value 0.146 days) and from 0.106 to 1.162 days (average value 0.131 days), respectively. Significant differences regarding virulence of the three isolates at p-level < 0.05 could not been proved. The other two examined isolates were distinctly less virulent to T. urticae than these three B. bassiana isolates.

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“…In the search for such methods, natural enemies are being evaluated as biological control agents of T. evansi (Wekesa et al 2005, Furtado et al 2007, Brito et al 2009. Another strategy that has been used where conventional pesticides are not allowed, such as in ecological and organic production systems, is the use of non synthetic products for pest control (Campanhola & Bettiol 2003, Venzon et al 2008a.…”

mentioning

confidence: 99%

Alternative control of Tetranychus evansi Baker & Pritchard (Acari: Tetranychidae) on tomato plants grown in greenhouses

Soto¹,

Venzon²,

Oliveira³

et al. 2010

Neotrop. entomol.

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-Tetranychus evansi Baker & Pritchard is an important pest of solanaceous plants, including tomatoes. This mite is characterized by a high reproductive rate, which leads to high population growth in a short period of time causing important economic damage. Control of T. evansi is mainly through synthetic acaricides. In searching for environmentally friendly control measures, we evaluated the effi ciency of alternative products to control T. evansi on tomato plants under greenhouse conditions. The products tested were lime sulphur and neem based products. We fi rst estimated the lethal concentration (LC) and instantaneous rate of increase (r i ) of T. evansi exposed to different product concentrations in laboratory conditions, and later tested the effi cacy of LC 95 and the concentrations that restrained mite population growth (r i = 0) in greenhouse conditions. The following treatments were repeated three times: NeemPro (81.0 and 71.6 mg a.i./l), Natuneem (31.1 and 20.4 mg ai/l), Organic Neem (39.1 and 30.4 mg a.i./l), lime sulphur (1.0 and 0.6%) and water (control). For all products, control provided by LC 95 was higher than provided for lower concentrations (r i = 0) one day after spraying. However, after fi ve days, for both concentrations, the percentage of T. evansi population reduction was superior to 95% and increased over time. Only plants sprayed with Natuneem (31.1 mg a.i./l) showed symptoms of phytotoxicity. Lime sulphur and neem based products, applied in appropriate concentrations and formulations, bear out as a viable alternative to control T. evansi on tomato plants.KEY WORDS: Acari, Azadirachta indica, lime sulphur, effi ciency, phytotoxicityThe tomato red spider mite, Tetranychus evansi Baker & Pritchard, is an important pest of solanaceous plants, especially tomatoes (Flechtmann 1983, Bonato 1999, Ferragut & Escudero 2002. This mite is characterized by a high reproductive capacity, which leads to high population levels in a short time, causing important economic damage (Moraes & McMurtry 1986). Additionally, the mite produces large quantities of webs on the infested plants, hampering the action of natural enemies (Gerson 1985, Sabelis & Bakker 1992. The control of T. evansi in tomato is done mainly with application of synthetic pesticides. Despite its relative effi ciency, chemical control has several negative impacts as the selection of resistant individuals due to the continuous use of certain active ingredients, the reduction or elimination of benefi cial species, the high toxicity of products to applicators, and the presence of residues in food (Picanço et al 2007, Maniania et al 2008.A viable alternative to the problems arising from excessive use of synthetic pesticides in tomato production is the use of methods that provide control with social and environmental safety. In the search for such methods, natural enemies are being evaluated as biological control agents of T. evansi (Wekesa et al 2005, Furtado et al 2007, Brito et al 2009. Another strategy that has been used where con...

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Pathogenicity of Beauveria bassiana and Metarhizium anisopliae to the tobacco spider mite Tetranychus evansi

Cited by 79 publications

References 19 publications

Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Alternative control of Tetranychus evansi Baker & Pritchard (Acari: Tetranychidae) on tomato plants grown in greenhouses

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