Pathogenicity and Characterization of Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus) in Kafr Elshikh Governorate

Saleh, Hosam M.; Sabry, Nader M.; Al-Razik, Mohamed Abd; Zahran, Faten; Ibrahim, Madiha S.

doi:10.5455/ajvs.243929

Cited by 6 publications

(11 citation statements)

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“…In the present study, presumptive identification of S . iniae was carried out from colony morphology on blood agar (Rahmatullah et al, 2017; Saleh et al, 2017). Phenotypic characters of the isolates were similar to those reported by Ortega et al (2018) and Rahmatullah et al (2017).…”

Section: Discussionmentioning

confidence: 99%

“…iniae among 150 fish samples that were collected during summer outbreaks. The high incidence of Streptococosis in summer may be attributed to the increase of water temperature (Saleh et al, 2017). High water temperature during summer is considered a stress factor, promoting the expression of various virulent factors, facilitating the invasion of Streptococcus to their hosts (Kayansamruaj et al, 2014; Rodkhum et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Efficacy ofBacillusprobiotic mixture on the immunological responses and histopathological changes of Nile tilapia (Oreochromis niloticus, L) challenged withStreptococcus iniae

Moustafa

Farrag

Dawood

et al. 2020

Aquaculture Research

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Isolates of Streptococcus iniae were recovered from the diseased fish during summer season, identified and characterized using different laboratory techniques. Three hundred and sixty Nile tilapia (100 ± 5.0 g average weight) were divided into two subgroups in triplicates with 20 fish per replicate and received either sterile PBS (B0, B1 and B2, respectively) or S. iniae (B0+ S. iniae, B1+ S. iniae and B2 S. iniae, respectively). At 15 days post‐challenge, results showed low mortality rates in probiotic‐fed groups. The mortalities ranged from 6.67% in B2 to 81.67% in B0 following S. iniae challenge. The haematological parameters showed a significant increase (p < 0.05) in haemoglobin, red blood cells and white blood cells in B1, B1+S. iniae, B2 and B2+ S. iniae groups compared to B0+ S. iniae group. The alanine aminotransferase (ALT) level was elevated (p < 0.05) in tilapia challenged with S. iniae compared to the control groups. Glucose and creatinine levels were elevated (p < 0.05) in all groups compared with B0. Both albumin and globulin levels were significantly low (p < 0.05) in B0+ S. iniae compared to other groups. The total protein level, phagocytosis and phagocytic index were significantly lower (p < 0.05) in B0+ S. iniae than other groups. Histological analysis showed that the hepatopancreas of B0, B1 and B2 groups represents normal hepatocyte architecture, while the infected tilapia showed severe diffused necrosis, mononuclear cell infiltration and loss of the normal architecture. Spleen of PBS control groups did not show any pathological changes, while that of infected tilapia revealed several necrotic areas in addition to marked depletion of lymphoid cells. A loss of skin layers was observed in infected tilapia with marked interstitial oedema and leucocytic cell infiltration which was not observed in the probiotic‐fed fish. The current study highlights the immunomodulatory effect of Bacillus probiotic mixture against S. iniae infection in tilapia that can be further applied to control the disease in farms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Efficacy ofBacillusprobiotic mixture on the immunological responses and histopathological changes of Nile tilapia (Oreochromis niloticus, L) challenged withStreptococcus iniae

Moustafa

Farrag

Dawood

et al. 2020

Aquaculture Research

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“…Nile Tilapia is a freshwater fish found to be one of the first species that have been cultured in the world and considered the most consumable fish in Egypt [ 4 ]. Tilapia represents about 65.2 % from the total fish production in Egypt in the year 2015 with 989,606 tons; about 88.47 % (875,513 tons) from them are produced from aquaculture while the other 11.53 % (114,093 tons) are obtained from the natural sources as reported in the statistics of fish production prepared by the General Authority for Fish Resources Development [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…These methods are characterized by high stockpile density and size, extensive use of formulated feed that contains antibiotics, antifungal, and other pharmaceutical preparations [ 5 , 2 ]. Fish bacterial diseases are one of the most vital problems facing the aquaculture industry in Egypt [ 4 ]. Therefore, antibiotics can intentionally be added to aquaculture either as prophylactic or as therapeutic to control fish diseases.…”

Section: Introductionmentioning

confidence: 99%

Incidence, stability and risk assessment for sulfonamides and tetracyclines in aqua-cultured Nile Tilapia fish of Egypt

et al. 2020

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“…Acute form of streptococcal infection in O. niloticus has resulted in more than 50% mortalities during about 3 days to one week while the chronic form may extend for several weeks with low rate daily mortalities about one or two percent (Osman et al, 2017). The clinical signs of streptococcal infection are including hemorrhagic gills, hemorrhagic patches on the abdominal wall and clouding of the cornea with exophthalmia in infected O. niloticus in addition to the most prominent postmortem lesions were pale enlarged liver with hemorrhagic enlarged spleen and presence of thick ascetic fluid and sometimes tinged with blood in the abdominal cavity (Saleh et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

Pathogenic characteristics and molecular detection of Streptococcus iniae isolated from infected Oreochromis niloticus

Saleh¹,

Ali²,

Aboyadak³

et al. 2019

Egypt. J. of Aquatic Biolo. and Fish.

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PathogenicityStreptococcosis became one of the most dangerous bacterial diseases affects cultured marine and freshwater fish. In the present study Streptococcus iniae was isolated from diseased Nile tilapia (Oreochromis niloticus) with investigation of the clinical signs, post mortem lesions and focusing on the histopathological changes in liver and kidney using the light and Transmission Electron Microscope (TEM). Natural infected O. niloticus showed ascites, exophthalmia, presence of hemorrhagic patches all over the external body surface with inflammation and swelling of the anal opening. Post-mortem examination indicated presence of hepatomegaly, splenomegaly, congestion and swelling of posterior kidney. Four Streptococcus iniae isolates were recovered and identified using PCR from the examined fish. The LD 50-96h of the recovered isolates in experimentally infected Oreochromis niloticus were ranged between 0.2 ml of 2.62 -3 × 10 7 CFU/ml by intraperitoneal injection. Under the light microscopical examination, severe degenerative changes were present in the hepatopancreas including diffused hepatic cell vacuolation and necrosis, leukocytic infiltration, the posterior kidney tissue showed vacuolation of tubular epithelial cells, shrinkage of glomerular taught and increasing the Bowman's space. TEM examination of hepatopancreas revealed the presence of hepatocellular degeneration, increased vacuolation in the different areas of the cytoplasm, loss of endoplasmic reticulum contact in several areas. Kidney tissues showed numerous vacuolation, swollen of cuboidal epithelial cell with absence of brush border in the apical portion of the proximal convoluted tubules, nucleus and mitochondria were dispersed throughout the cytoplasm.

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Pathogenicity and Characterization of Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus) in Kafr Elshikh Governorate

Cited by 6 publications

References 0 publications

Efficacy ofBacillusprobiotic mixture on the immunological responses and histopathological changes of Nile tilapia (Oreochromis niloticus, L) challenged withStreptococcus iniae

Efficacy ofBacillusprobiotic mixture on the immunological responses and histopathological changes of Nile tilapia (Oreochromis niloticus, L) challenged withStreptococcus iniae

Incidence, stability and risk assessment for sulfonamides and tetracyclines in aqua-cultured Nile Tilapia fish of Egypt

Pathogenic characteristics and molecular detection of Streptococcus iniae isolated from infected Oreochromis niloticus

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