Pathogenicity and characterization of a novel Bacillus cereus sensu lato isolate toxic to the Mediterranean fruit fly Ceratitis capitata Wied.

The scientific community working in the field of insect pathology is experiencing an increasing academic and industrial interest in the discovery and development of new bioinsecticides as environmentally friendly pest control tools to be integrated, in combination or rotation, with chemicals in pest management programs. In this scientific context, market data report a significant growth of the biopesticide segment. Acquisition of new technologies by multinational Ag-tech companies is the center of the present industrial environment. This trend is in line with the requirements of new regulations on Integrated Pest Management. After a few decades of research on microbial pest management dominated by Bacillus thuringiensis (Bt), novel bacterial species with innovative modes of action are being discovered and developed into new products. Significant cases include the entomopathogenic nematode symbionts Photorhabdus spp. and Xenorhabdus spp., Serratia species, Yersinia entomophaga, Pseudomonas entomophila, and the recently discovered Betaproteobacteria species Burkholderia spp. and Chromobacterium spp. Lastly, Actinobacteria species like Streptomyces spp. and Saccharopolyspora spp. have gained high commercial interest for the production of a variety of metabolites acting as potent insecticides. With the aim to give a timely picture of the cutting-edge advancements in this renewed research field, different representative cases are reported and discussed.

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“…, Cyt, VIP) [ 3 ]. The insecticidal potential of bacterial strains closely related to B. thuringiensis has also been demonstrated [ 11 ].…”

Section: Insect Pathogenic Bacteriamentioning

confidence: 99%

Insect Pathogenic Bacteria in Integrated Pest Management

Ruiu

2015

Insects

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160

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“…Among these are the insecticidal crystal toxins (Cry) typical of Bacillus thuringiensis and Lysinibacillus sphaericus , and the insecticidal toxin complexes of gammaproteobacteria like the entomopathogenic nematode symbionts Photorhabdus and Xenorhabdus species (Tc), Serratia species (Sep), and Yersinia entomophaga (Yen-Tc)7. In certain cases, insect virulence factors are localised on the outermost spore layers as demonstrated in the Bacillus cereus group8910.…”

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confidence: 99%

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

Marche

Mura

Falchi

et al. 2017

Sci Rep

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Outer spore envelope proteins of pathogenic bacteria often present specific virulence factors and tools to evade the defence system of their hosts. Brevibacillus laterosporus, a pathogen of invertebrates and an antimicrobial-producing species, is characterised by a unique spore coat and canoe-shaped parasporal body (SC-CSPB) complex surrounding the core spore. In the present study, we identified and characterised major proteins of the SC-CSPB complex of B. laterosporus, and we investigated their entomopathogenic role. Employing a proteomic approach and a B. laterosporus-house fly study model, we found four highly conserved proteins (ExsC, CHRD, CpbA and CpbB) that function as insect virulence factors. CpbA was associated with a significantly higher mortality of flies and greater relative gene expression levels during sporulation, compared to the other SC-CSPB proteins. Taken together, we suggest that spore surface proteins are a part of a complex set of toxins and virulence factors that B. laterosporus employs in its pathogenicity against flies.

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“…Previous studies suggested that the bacterial toxic effect interfered with the feeding behavior and nutritional physiology of insects to influence their development and reproduction (Nian et al, ; Ruiu et al, ; Thakur, Dhammi, Saini, & Kaur, ). For example, the relative consumption and growth rate of Spodoptera litura fed on Enterobacter cloacae ‐treated diet showed a significant decrease compared with control larvae (Thakur et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, to attain the minimum weight requirement for pupation, insects prolonged its larval period. For example, feeding on the sporulated cultures of B. cereus significantly extended development time, reduced immature survival, pupal weight, and adult emergence of C. capitate (Ruiu et al, ). Similarly, the oral ingestion of Brevibacillus laterosporus significantly caused high sublethal effects on the larvae, reduced adult size, and impaired the reproductive performance of Musca domestica (Zimmer et al, ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of Pseudomonas cedrina infecting Plutella xylostella (Lepidoptera: Plutellidae)

Liu

Lin

Kang

et al. 2019

Arch Insect Biochem Physiol

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The diamondback moth, Plutella xylostella, is one of the most destructive pests worldwide and its management relies exclusively on frequent application of chemical insecticides. Resistance to common insecticides is now widespread, and novel classes of insecticides are needed.Entomopathogenic bacteria and their related products play an important role in the management of this pest.In the present work, one bacterial strain was separated from infected pupae of P. xylostella collected from field and its pathogenicity was evaluated. On the basis of the 16S ribosomal RNA sequencing, BLASTN, and phylogenetic analysis, this bacterial isolate was identified as Pseudomonas cedrina. Oral administration of P. cedrina at levels above 10,000 CFU/ml gave significant mortality to P. xylostella larvae. The pathogenicity was also observed by reduced longevity and fecundity in adult females. However, when live bacterial cells were removed, the cultured broth lost any pathogenicity. In response to the bacterial infection, P. xylostella expressed antimicrobial and stress-associated genes. A mixture treatment of P. cedrina and Bacillus thuringiensis showed an additive effect on larval mortality of P. xylostella. These results indicated that P. cedrina is an opportunistic entomopathogen without secretion of toxins. Furthermore, the additive effect of P. cedrina and B. thuringiensis provide a new insight to develop new strategy for controlling P. xylostella. K E Y W O R D S additive effect, opportunistic pathogen, Plutella xylostella, Pseudomonas cedrina

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Pathogenicity and characterization of a novel Bacillus cereus sensu lato isolate toxic to the Mediterranean fruit fly Ceratitis capitata Wied.

Cited by 25 publications

References 41 publications

Insect Pathogenic Bacteria in Integrated Pest Management

Insect Pathogenic Bacteria in Integrated Pest Management

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

Isolation and characterization of Pseudomonas cedrina infecting Plutella xylostella (Lepidoptera: Plutellidae)

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