Isolation and characterization of Pseudomonas cedrina infecting Plutella xylostella (Lepidoptera: Plutellidae)

Abstract

The diamondback moth, Plutella xylostella, is one of the most destructive pests worldwide and its management relies exclusively on frequent application of chemical insecticides. Resistance to common insecticides is now widespread, and novel classes of insecticides are needed.Entomopathogenic bacteria and their related products play an important role in the management of this pest.In the present work, one bacterial strain was separated from infected pupae of P. xylostella collected from field and its pathogenicity was evaluated. On the basis of the 16S ribosomal RNA sequencing, BLASTN, and phylogenetic analysis, this bacterial isolate was identified as Pseudomonas cedrina. Oral administration of P. cedrina at levels above 10,000 CFU/ml gave significant mortality to P. xylostella larvae. The pathogenicity was also observed by reduced longevity and fecundity in adult females. However, when live bacterial cells were removed, the cultured broth lost any pathogenicity. In response to the bacterial infection, P. xylostella expressed antimicrobial and stress-associated genes. A mixture treatment of P. cedrina and Bacillus thuringiensis showed an additive effect on larval mortality of P. xylostella. These results indicated that P. cedrina is an opportunistic entomopathogen without secretion of toxins. Furthermore, the additive effect of P. cedrina and B. thuringiensis provide a new insight to develop new strategy for controlling P. xylostella. K E Y W O R D S additive effect, opportunistic pathogen, Plutella xylostella, Pseudomonas cedrina

Phenyl imidazolidin‐2‐ones antagonize a β‐adrenergic‐like octopamine receptor in diamondback moth (Plutella xylostella)

“…The diamondback moth (Plutella xylostella) is one of the most destructive lepidopteran pests on cruciferous vegetables around the world. 4 Approximately US $0.77 billion are spent controlling this pest species per year in China, and recent studies have focused on its biological and chemical control strategies. 5 Additionally, resistance has emerged to the current chemical and biological insecticides used for P. xylostella control.…”

Bacteria in the oral cavity of individuals consuming intoxicating substances

“…K. michiganensis is an emerging multidrug-resistant human pathogen [76]. However, P. cedrina, a bio-pesticide against Plutella xylostella [77] and reported as antiproliferative against human cervical carcinoma cell lines Hela Lung A-549 (HBL-100) [78]. The present findings corroborate the bacterial characterization in betel quid chewer and non-chewer by Deepak et al [34], poor oral hygiene and chronic periodontitis in betel nut chewers [79], microbial association with dysbiosis and increased risk of oral cancer [35].…”

Uji Efikasi Beauveria Bassiana Untuk Pengendalian Hama Ulat Plutella Xylostella Pada Tanaman Kale (Brassica Oleracea) Var. Sabellica

“…Dalam kegiatan budidaya tanaman sawi-sawian selalu terdapat beberapa kendala antara lain gangguan serangga fase ulat dari ordo Lepidoptera yaitu Pluttela xylostella. Hama ini sering ditemukan hidup berkelompok di permukaan daun dan merusak berbagai jenis sayuran di seluruh dunia (Liu et al, 2019). Dalam pengendalian hama ulat daun yang merugikan ini selalu digunakan pestisida kimia.…”