Pathogenic potential of Brucella ovis field isolates with different genotypic profile and protection provided by the vaccine strain B. ovis ΔabcBA against B. ovis field isolates in mice

Carvalho, Thaynara Parente de; Ribeiro, Noelly Queiroz; Mol, Juliana Pinto da Silva; Costa, Fabíola Barroso; Eckstein, Camila; Paula, Nayara Ferreira de; Paixão, Tatiane Alves da; Santos, Renato L.

doi:10.1590/1678-5150-pvb-6481

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…22 Previous studies demonstrated that BoΔabcBA protects against experimental challenge with virulent Brucella strains. [17][18][19] Silva et al 17 found a protection index of approximately 1 when C57BL/6 mice immunized with alginate-encapsulated BoΔabcBA were challenged with virulent B. ovis, although protection indexes tend to increase when mice are challenged with more virulent B. ovis field isolates, 28 whereas Eckstein et al 19 reported a 1.5 protection index in response to an experimental challenge with wild-type B. canis. It is noteworthy that although the mouse model is a broadly used tool for assessing vaccine protective potential, it cannot precisely predict the degree of protection that can be achieved when the vaccine is administered to Brucella-preferential host species.…”

Section: Discussionmentioning

confidence: 99%

“…Silva et al 17 . found a protection index of approximately 1 when C57BL/6 mice immunized with alginate‐encapsulated Bo ∆ abcBA were challenged with virulent B. ovis , although protection indexes tend to increase when mice are challenged with more virulent B. ovis field isolates, 28 whereas Eckstein et al 19 . reported a 1.5 protection index in response to an experimental challenge with wild‐type B. canis .…”

Section: Discussionmentioning

confidence: 99%

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The candidate vaccine strain Brucella ovis ∆abcBA is protective against Brucella melitensis infection in mice

Costa

Cabello

Batista

et al. 2020

Microbiology and Immunology

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Brucellosis is a major zoonotic disease, and Brucella melitensis is the species most often associated with human infection. Vaccination is the most efficient tool for controlling animal brucellosis, with a consequent decrease of incidence of human infections. Commercially available live attenuated vaccines provide some degree of protection, but retain residual pathogenicity to human and animals. In this study, Brucella ovis ΔabcBA (BoΔabcBA), a live attenuated candidate vaccine strain, was tested in two formulations (encapsulated with alginate and alginate plus vitelline protein B [VpB]) to immunize mice against experimental challenge with B. melitensis strain 16M. One week after infection, livers and spleens of immunized mice had reduced numbers of the challenge strain B. melitensis 16M when compared with those of nonimmunized mice, with a reduction of approximately 1-log 10 of B. melitensis 16M count in the spleens from immunized mice. Moreover, splenocytes stimulated with B. melitensis antigens in vitro secreted IFN-γ when mice had been immunized with BoΔabcBA encapsulated with alginate plus VpB, but not with alginate alone. Body and liver weights were similar among groups, although spleens from mice immunized with BoΔabcBA encapsulated with alginate were larger than those immunized with BoΔabcBA encapsulated with alginate plus VpB or nonimmunized mice. This study demonstrated that two vaccine formulations containing BoΔabcBA protected mice against experimental challenge with B. melitensis.

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