Pathogenesis of Brucella ovis in pregnant mice and protection induced by the candidate vaccine strain B. Ovis ΔabcBA

A Review on the Methodology and Use of the Pregnant Mouse Model in the Study of Brucella Reproductive Pathogenesis and Its Abortifacient Effect

“…In contrast, although few brucellae localised in the placenta early in the infection, the organ rapidly became the main focus of bacterial replication, evidencing the remarkable tropism of Brucella for this organ [62]. This aspect of an asymmetrical placenta/spleen infection was also highlighted in later publications after IP inoculation of B. abortus 544 [39,66,67], B. abortus 2308 [37,38], B. melitensis NI [40], B. melitensis biovar 1 strain 16M and Rev1 [73][74][75], and B. ovis [76]. Interestingly, the higher degree of placental colonisation did not result in an equally high foetal infection [40,63], evidencing the invasion-limiting barrier function of the placenta [86].…”

“…Then, these authors used their model to evaluate the reproductive safety of two vaccine candidates on the B. melitensis 16M-and Rev1-backgrounds [74,75]. Coincidentally to these studies, the pregnant mouse was also used to study the abortifacient effect of the B. ovis strain ATCC ® 25840 as well as a B. ovis-based vaccine candidate (Table 1) [76].…”

Comparative study on alginate/chitosan microcapsules and Montanide ISA 61 as vaccine adjuvants in mice

“…Positive controls included tissue sections from mice experimentally infected with Brucella sp. [ 17 ]. Negative controls had the primary antibody replaced with sterile PBS.…”

Antigenic and molecular evidence of Brucella sp.-associated epididymo-orchitis in frugivorous (Artibeus lituratus) and nectarivorous (Glossophaga soricina) bats in Brazil

“…Sections were then incubated with a polyclonal anti- Brucella spp. antibody and diluted in the ratio of 1:1000 in 1% bovine serum albumin (BSA) for 1 h. The primary antibody employed in this study has been previously developed and characterized in our laboratory, as described in Xavier et al ( 11 ), and it has been demonstrated to cross-react with various Brucella species ( 11 , 20 , 21 ). Sections were rinsed three times in PBS and incubated with a detection system (EnVision FLEX+; Dako, Carpinteria, CA, USA) for 30 min, followed by rinsing in PBS and development with chromogen 3′3-diaminobenzidine (DAB+ Substrate Chromogen; Dako) for 80 s, and sections were counterstained with Mayer's hematoxylin for 35 s. Positive controls included tissue sections from mice experimentally infected with Brucella ovis .…”