Pathogenic ‘Bison-type’ Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India

Yadav, D.; Singh, Shoor Vir; Singh, Ajay Vir; Sevilla, Iker A.; Juste, Ramón A.; Singh, Pravin Kumar; Sohal, J.S.

doi:10.1016/j.cimid.2007.06.007

Cited by 45 publications

(29 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Knowing the status of MAP and the genotypes of MAP present in different species and geographic areas is a requisite to formulating policies for developing diagnostics and disease control strategies (Yadav et al, 2008). There are few reports about Johne's disease in wild boars.…”

mentioning

confidence: 99%

Mycobacterium avium paratuberculosis in Wild Boars in Korea

Kim

Lee

et al. 2013

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic infectious enteritis in various domestic and wild mammals and is widely distributed globally. Interspecies transmission has been frequently reported. We investigated the presence of MAP from December 2010 to March 2011 in blood and feces collected from 222 hunter-killed wild boars. We collected 197 serum and 180 fecal samples and examined them by culture, PCR, and enzyme-linked immunosorbent assay. We investigated the status of MAP infection and the MAP genotypes in the wild boar population of Korea by using IS900 PCR and IS1311-restriction endonuclease analysis typing. Of the 180 fecal samples cultured, MAP colonies were recovered from two. By PCR, 18 animals were positive for MAP and one serum sample had a strong humoral response to MAP. The PCR-positive DNA samples from the colonies and the feces samples were genotyped as ''cattle type'' and ''bison type,'' which are major MAP genotypes infecting domestic species in Korea. Our study provides new information on mycobacterial infection among wild boars, and suggests that a more effective program should be developed to monitor mycobacterial infections in wild animal populations in Korea.

show abstract

mentioning

confidence: 99%

Mycobacterium avium paratuberculosis in Wild Boars in Korea

Kim

Lee

et al. 2013

Journal of Wildlife Diseases

View full text Add to dashboard Cite

show abstract

“…Singh et al, (2007b) evaluated i_ELISA kit with commercial purified PPA of Allied Monitor Inc., USA and commercial ELISA kit (Pourquier, France) in goats and sheep with culture and reported that as compared to type II reactors, type 1 reactors in i_ELISA had maximum correlation with culture. Yadav et al, (2008) first time standardized goat based "indigenous ELISA kit" (Singh et al, 2007b), for sero-screening of unproductive buffaloes slaughtered for meat production from Agra region of South Uttar Pradesh (UP) and reported similar high (46.7%) sero-incidence of MAP infection. Using culture of tissues (MLN and large intestine) 48.0% buffaloes were positive and 40% were positive by IS900 PCR.…”

Section: Discussionmentioning

confidence: 91%

“…In rural dairy population, the Incidence of MAP was lower than organized dairy farms. Tripathi et al (2007) screened sera of 320 buffaloes [Central West India (80), Northern India (240) Yadav et al, in 2008, using cPPA (Commercial PPA) from MAP of bovine origin from Allied Monitor Inc., USA, none of the buffalo was positive, whereas using sPPA from MAP "Indian Bison Type" strain "S 5" of goat origin 46.7% buffaloes were found positive. Similarly in the present study also, of 156 serum samples screened, 41.0% were found positive by "Indigenous ELISA kit".…”

Section: Discussionmentioning

confidence: 99%

Bio-incidence of bovine Johne’s disease in dairy buffaloes in Central and North India using sensitive goat based ‘indigenous elisa kit’ and traditional tests

Audarya¹,

Chhabra²,

Sahzad³

et al. 2016

JEBAS

View full text Add to dashboard Cite

Present study was aimed to estimate the bio-incidence of Bovine Johne"s disease in native population of buffaloes (Murrah, Zaffarabadi and Bhadawari) in dairy farms and farmer"s herds located in Central and North India. Total 156 serum samples were screened from a dairy herd in Kiratpur (Malwa region), and 41.0% buffaloes were positive for Mycobacterium avium subsp. paratuberculosis (MAP) infection using "Indigenous ELISA kit". In another study, 101 young males (farmer"s herds) from the native tract (Jind / Rohtak, Haryana) of Murrah breed were screened and 80.1% were reported positive by kit. In a farm herd of Bhadawari buffaloes in its native tract (Etawah district in Uttar Pradesh) and dairy herd of Murrah buffaloes procured from native tract and kept in Mathura district of Uttar Pradesh, In the two farms 20.0% buffaloes were screened and 71.4% were positive by fecal Microscopy. Study reported high bio-incidence of BJD in native breeds of buffaloes in Malwa region of Central India, Etawah and Mathura (Uttar Pradesh) and Jind / Rohtak (Haryana) regions of North India, despite high slaughter rate, for meat harvesting. Goat based 'Indigenous ELISA kit' was employed for screening since commercial kits were costly for the screening of buffaloes. Moreover Imported 'ELISA kits' were based on PPA from 'Cattle Type' biotype', a different strain from other geographical regions / country. "Indigenous ELISA kit" using native 'Indian Bison type' biotype was efficient, specific and cost effective 'mass screening' test to estimate bio-incidence of BJD in large population of buffaloes at National level.

show abstract

“…Screening of milk samples of domestic livestock using ELISA kit, bio-load of MAP in 'raw milk samples' was also high (25.0-60.0%) (un-published data of our lab). Earlier studies also reported high bio-load of MAP in domestic livestock using milk and it was 58.2 to 70.1% in goats (Kumar et al, 2008), 32.1% in cattle and 46.8% in buffaloes (Yadav et al, 2008). Since MAP is not inactivated during pasteurization (Lund et al, 2002), therefore to reduce contamination of MAP in milk and milk products it is essential to control infection in animals.…”

Section: Resultsmentioning

confidence: 99%