Pathogenic and genetic variability in Tilletia indica monosporidial culture lines using universal rice primer-PCR

Aggarwal, Rashmi; Tripathi, Aradhika; Yadav, Anita

doi:10.1007/s10658-010-9655-4

Cited by 27 publications

(21 citation statements)

References 16 publications

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“…Our results on pathogenic variability and previous results (Singh et al 1995;Bonde et al 1996;Aggarwal et al 2011) on host pathogen interaction have indicated that there is no well-defined race concept in this pathogen as it exists in the wheat rusts. However, four distinct pathotypes were identified among the isolates of KB collected from Punjab and Himachal Pradesh (Aujla et al 1987).…”

contrasting

confidence: 42%

Determination of genetic and pathological variance amongTilletia indicaisolates and monosporidial lines using PCR based markers and host differentials

Parveen

Saharan

Verma

et al. 2013

Archives Of Phytopathology And Plant Protection

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Karnal bunt of wheat caused by Tilletia indica is an important international quarantine disease in many countries. In this investigation, genetic and pathological variation among the 10 isolates and 15 monosporidial (Ms) lines belonged to different locations of North-West India was studied. Depending upon the pathogenic potential, most virulent and least aggressive isolate was found from Chaksu (Rajasthan) and Tarau (HP), which scored coefficients of infection 70.98 and 6.22, respectively, on susceptible host genotype HD 2009 under artificially inoculated conditions. Fifteen Ms lines were inoculated in 20 combinations. Most virulent compatible combination was found KB2MsD Â KB6MsA, which scored co-efficient of infection 74.91%. Out of 32 Inter Simple Sequence Repeats based molecular markers, 28 were polymorphic generating 192 reproducible bands for all the T. indica isolates and Ms lines in this study. A grouping analysis using the unrooted neighbour -joining method was consistent with DARwin software and winboot analysis and combination approach suggested that self-paired Ms lines exhibit narrow genetic diversity. This result will be useful for developing integrated strategies for disease management and breeding programmes for improvement of the varieties.

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confidence: 42%

Determination of genetic and pathological variance amongTilletia indicaisolates and monosporidial lines using PCR based markers and host differentials

Parveen

Saharan

Verma

et al. 2013

Archives Of Phytopathology And Plant Protection

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“…This suggests that ‘hot spot’ areas of Karnal bunt are shifting from one place to another in the Karnal bunt prevailing areas. During its annual cycle, the pathogen reproduces sexually, resulting in assortment of virulence genes resulting in the pathogen in a population showing continuous variation at any time without distinct races (Singh and Srivastava 1992 and Aggarwal et al. 2010).…”

Section: Discussionmentioning

confidence: 99%

Variability of Indian Isolates of Tilletia indica Assessed by Pathogenicity and Molecular Markers

Thirumalaisamy

Singh

2012

Journal of Phytopathology

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Twenty isolates of Tilletia indica collected from sites in North and North‐western India showed pathogenic variation on 18 host differentials. Sixteen aggressive pathotypes were identified on the basis of percent coefficient of infection (PCI). Two major clusters were apparent in the dendrogram; cluster 1 comprised 13 isolates and cluster two consisted of seven isolates. One of the isolate Kashipur had a high PCI on most of the host differentials compared to other isolates. Polymerase chain reaction‐based random amplified polymorphic DNA (PCR – RAPD) analysis also divided isolates into two major clusters, one comprising of 5 isolates collected from hill and foot‐hill sites and another group comprising of 15 isolates collected from plain sites. Thus, the clusters identified based on PCI did not match closely with those identified by molecular analysis based on RAPD. Although diversity among the isolates of T. indica was absent in the rDNA‐ITS region, our study based on pathogenicity and molecular markers confirms the existence of great diversity in the pathogen, also shifting of ‘hot spot’ areas from one place to another within Karnal bunt prevailing areas.

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“…; Aggarwal et al. ). Therefore, URPs were also selected for studying the molecular diversity of Foc along with other molecular markers such as RAPD, ISSR and SSR.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Dubey et al (2010b) developed ITS regionbased markers for the detection of Indian isolates of Foc. Universal rice primers (URPs) have been used to fingerprint diverse genomes and to determine the genetic variability of plant pathogenic fungi (Kang et al 2002;Sharma et al 2005;Aggarwal et al 2010). Therefore, URPs were also selected for studying the molecular diversity of Foc along with other molecular markers such as RAPD, ISSR and SSR.…”

Section: Introductionmentioning

confidence: 99%

Race Profiling and Molecular Diversity Analysis ofFusarium oxysporumf.sp.cicerisCausing Wilt in Chickpea

Dubey

Kumari

Singh

et al. 2012

Journal of Phytopathology

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Seventy isolates of Fusarium oxysporum f.sp. ciceris (Foc) causing chickpea wilt representing 13 states and four crop cultivation zones of India were analysed for their virulence and genetic diversity. The isolates of the pathogen showed high variability in causing wilt incidence on a new set of differential cultivars of chickpea, namely C104, JG74, CPS1, BG212, WR315, KWR108, GPF2, DCP92‐3, Chaffa and JG62. New differential cultivars for each race were identified, and based on differential responses, the isolates were characterized into eight races of the pathogen. The same set of isolates was used for molecular characterization with four different molecular markers, namely random amplified polymorphic DNA, universal rice primers, simple sequence repeats and intersimple sequence repeats. All the four sets of markers gave 100% polymorphism. Unweighted paired group method with arithmetic average analysis grouped the isolates into eight categories at genetic similarities ranging from 37 to 40%. The molecular groups partially corresponded to the states of origin/chickpea‐growing region of the isolates as well as races of the pathogen characterized in this study. The majority of southern, northern and central Indian populations representing specific races of the pathogen were grouped separately into distinct clusters along with some other isolates, indicating the existence of variability in population predominated by a single race of the pathogen. The present race profiling for the Indian population of the pathogen and its distribution pattern is entirely new. The knowledge generated in this study could be utilized in resistance breeding programme. The existence of more than one race, predominated by a single one, in a chickpea cultivation zone as supported by the present molecular findings is also a new information.

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Pathogenic and genetic variability in Tilletia indica monosporidial culture lines using universal rice primer-PCR

Cited by 27 publications

References 16 publications

Determination of genetic and pathological variance amongTilletia indicaisolates and monosporidial lines using PCR based markers and host differentials

Determination of genetic and pathological variance amongTilletia indicaisolates and monosporidial lines using PCR based markers and host differentials

Variability of Indian Isolates of Tilletia indica Assessed by Pathogenicity and Molecular Markers

Race Profiling and Molecular Diversity Analysis ofFusarium oxysporumf.sp.cicerisCausing Wilt in Chickpea

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