Pathogenic Acinetobacter species including the novel Acinetobacter dijkshoorniae recovered from market meat in Peru

Abstract

Species of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex are important human pathogens which can be recovered from animals and food, potential sources for their dissemination. The aim of the present study was to characterise the Acinetobacter isolates recovered from market meat samples in Peru. From July through August 2012, 138 meat samples from six traditional markets in Lima were cultured in Lysogeny and Selenite broths followed by screening of Gram-negative bacteria in selective media. Bacterial isolates were identified by MALDI-TOF MS and DNA-based methods and assessed for their clonal relatedness and antimicrobial susceptibility.Twelve Acinetobacter isolates were recovered from calf samples. All but one strain were identified as members of the clinically-relevant Acinetobacter calcoaceticus-Acinetobacter baumannii complex: 9 strains as Acinetobacter pittii, 1 strain as A. baumannii, and 1 strain as the recently described novel species A. dijkshoorniae.The remaining strain could not be identified at the species level unambiguously but all studies suggested close relatedness to A. bereziniae. All isolates were well susceptible to antibiotics. Based on macrorestriction analysis, six isolates were further selected and some of them were associated with novel MLST profiles.The presence of pathogenic Acinetobacter species in human consumption meat might pose a risk to public health as potential reservoirs for their further spread into the human population. Nevertheless, the Acinetobacter isolates from meat found in this study were not multidrug resistant and their prevalence was low. To our knowledge, this is also the first time that the A. dijkshoorniae species is reported in Peru.

From food to hospital: we need to talk about Acinetobacter spp.

“…35,36 However, other reports describe the species of Acinetobacter as potential pathogens and are concerned about their detection in foods, including samples of bovine milk, goats and dairy products. [37][38][39] The presence of pathogenic Acinetobacter species in meat for human consumption has been reported by Marí-Almirall and coworkers; 40 although the authors did not find multiresistant strains in their study, they report the risk that this may pose to public health, as these foods may act as potential reservoirs for the spread of Acinetobacter in the human population. Klotz and colleagues, 41 for instance, isolated carbapenem-resistant A. indicus strains in cattle and made an important observation: despite the low pathogenicity of this species, these isolates may contribute to the dissemination of resistance genes to other bacterial species and also to the environment through manure, contributing to the contamination of vegetables used for human consumption.…”

Description of Acinetobacter kanungonis sp. nov., based on phylogenomic analysis

“…Presently, the genus Acinetobacter comprised of 65 species with validly published names (https://lpsn.dsmz.de/genus/acinetobacter; last accessed February 4, 2021). The members of this genus are widely distributed in diverse environments, including water, soil, clinical specimens and animal food products like milk and meat [3–6].…”

Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health

“…In this study, the presence of ST1258 was detected in JLW ( 6 ), TXT ( 11 ), JN ( 9 ), TDY1 ( 8 ), and TDY2 ( 8 ). As the most common type of ST strains from swines, ST1258 has not been found in the related literature of E. coli , while it has been detected in Bacillus cereus ( 51 ), Acinetobacter dijkshoorniae ( 52 ), and Streptococcus pneumoniae ( 53 ). There is an intimation that attention should be paid to the epidemic of the type ST1258.…”