Pathogenesis-Related Proteins in Grape

Enoki, Shinichi; Suzuki, Shunji

doi:10.5772/64873

Cited by 14 publications

(12 citation statements)

References 69 publications

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“…Often PR proteins are highly expressed during osmotic stress conditions. In accordance with our results, PR proteins are reported to be highly expressed during the somatic embryo development of various species like Vitis vinifera 30 , Vitis pseudoreticulata 31 , Cichorium 29 and Pinus nigra Arn. 32 .…”

Section: Discussionsupporting

confidence: 92%

Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)

Krishnaveni

Uma

Backiyarani

et al. 2020

Sci Rep

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asynchronous developments pose limitation for its wider application. But, understanding the molecular mechanism of somatic embryo development may help in overcoming these limitations and assist commercial exploitation of this phenomenon. Proteins are mainly responsible for the biological function and phenotype of the cells 10,11. Proteomic approach has been considered as a powerful tool for examining the physiological condition of plant tissues, and organs, under specific developmental processes 12. Since proteins are directly involved in cellular biochemistry, identification of proteins related to the embryo development may reveal the molecular basis of SE 13. Research on the development of somatic embryo has been carried out over three decades, but most of the studies focused on physiological aspects and improvement of culture practice. More recently efforts have been put forth to examine the process of somatic embryo development at the molecular level. Proteomic analysis of different developmental stages of embryos were carried out in many commercial crops like Citrus sinensis Osbeck 13 , Coffea arabica 1,14 , Oryza sativa 10 , Manihot esculenta 15 , Picea glauca 16 , Carica papaya L. 9 , Cyclamen persicum 17 , Araucaria angustifolia 18 , Fraxinus mandshurica 2 and Acca sellowiana 4. Most of the study revealed that the formation and development of somatic embryos follow complex metabolic process and involve many proteins that are associated with the growth and developmental pathway. Understanding the molecular basis of somatic embryo development along with signaling pathways can provide insight into the growth and developmental process. Therefore, the study on proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA) was carried out with the main objective to examine and characterize the differential proteins expressed during various developmental stages of somatic embryo. The determination of differential protein expression will provide a novel insight into banana somatic embryo development and helps in the improvement of SE protocols in recalcitrant banana varieties. Materials and Methods plant materials and sample collection. The embryogenic cell suspension (cell line accession no: NGFB0189) generated from male flower bud of cv. Grand Naine (AAA) as per the procedure described by Kumaravel et al. 7 was used as an initiating material. After checking the viability of six months old embryogenic cell suspension using fluroscein diacetate (FDA) stain, one mL settled cell volume (SCV) of fine yellowish white homogenous suspension was plated on the somatic embryo regeneration medium. This was supplemented with 80 µg/L kinetin, 200 µg/L napthaleneacetic acid and 40 µg/L zeatin, as suggested in INIBAP technical guidelines 19 and maintained in complete darkness at 25 ± 2 °C for 60 days. Five plates of somatic embryos were used as replication. Embryos were collected in triplicates at 0, 30, 45 and 60 days after initiation of embryogenic cells on the regeneration medium. Samples collected were...

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Section: Discussionsupporting

confidence: 92%

Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)

Krishnaveni

Uma

Backiyarani

et al. 2020

Sci Rep

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“…The expression level of genes encoding PR‐2 (β‐1,3‐glucanases), PR‐3 (chitinases), PR‐5 (thaumatin‐like proteins) and the PR‐protein 10.3 were increased upon the treatment with B. bassiana within 24 h after treatment. These results confirm previous reports that these genes are involved in the defence response of grapevines to infestation with pathogens (Kortekamp, ; Fung et al , ; Albertazzi et al , ; Adrian et al , ; Enoki & Suzuki, ) and are also related in response to colonization by biotrophic fungi (Perazzolli et al , ). In addition, other defence‐related genes like those associated to the stilbene synthesis and related genes were found to be up‐regulated.…”

Section: Discussionsupporting

confidence: 92%

Endophytic Beauveria bassiana activates expression of defence genes in grapevine and prevents infections by grapevine downy mildew Plasmopara viticola

Rondot

Reineke

2019

Plant Pathology

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Fungal entomopathogens like Beauveria bassiana (Ascomycota: Hypocreales) are known as antagonists of insects with multiple functional and ecological roles, and have attracted increased attention as biocontrol agents in integrated pest management programmes. For some crop plants, it has been proven that endophytic B. bassiana, besides its entomopathogenic habit, can provide protection against plant pathogens or limit their damaging effects. However, for grapevine, limited knowledge is available on the influence of endophytic B. bassiana on fungal pathogens and about the mechanisms underlying putative protection effects. This study assessed the protective potential of endophytic B. bassiana against grapevine downy mildew Plasmopara viticola in greenhouse experiments. Three and seven days after a B. bassiana treatment, potted grapevine plants were inoculated with P. viticola and the evolving disease severity was assessed. Disease severity was significantly reduced in B. bassiana‐treated plants compared to control plants, depending on the age of leaves. Furthermore, a microarray and an RT‐qPCR analysis were performed to work out fundamental aspects of genes involved in the interaction between grapevine and B. bassiana. The results indicate an up‐regulation of diverse defence‐related genes in grapevine as a response to endophytic establishment of B. bassiana. Thus, endophytic establishment of an entomopathogenic fungus such as B. bassiana in grapevine plants would represent an alternative and sustainable plant protection strategy, with the potential for reducing pesticide applications in viticulture.

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“…According to molecular structure and enzyme activity, 17 families of PR proteins have been reported, some of which possess antimicrobial activity. Among them, PR‐1 (antifungal), PR‐2 (β‐1,3‐glucanase), PR‐3 and PR‐4 (chitinase), PR‐5 (thaumatin‐like protein), PR‐10 (ribonuclease), PR‐14 (lipid transfer protein), PR‐15 (oxalate oxidase), and PR‐16 (oxalate oxidase like protein) have been detected in grapevine (Enoki and Suzuki, 2016). Several research studies have demonstrated that these PR proteins are strongly induced in grapevine by fungal plant pathogens (Jacobs et al , 1999; Jayasankar et al , 2003; Godfrey et al , 2007; He et al , 2013).…”

Section: Introductionmentioning

confidence: 99%

Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine

et al. 2020

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Crown gall disease of grapevine, caused by Agrobacterium tumefaciens, often results in severe economic loss to grape production worldwide. This study demonstrated the ability of the endophytic bacteria Pseudomonas sp. Sn48 and Pantoea sp. Sa14 isolated from domesticated and wild grapevines to induce resistance in both above‐ and belowground tissues of grapevines infected with A. tumefaciens. Our results provide evidence that both strains can colonize roots and/or shoots. We showed that the strains Pseudomonas sp. Sn48 and Pantoea sp. Sa14 are capable of inducing stilbenic phytoalexin production in grapevine tissues and to further prime plantlets for enhanced phytoalexin production after A. tumefaciens inoculation. We also showed that in the majority of treatments, polyamine accumulation remained unchanged or slightly increased in plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 compared with the control. Our findings indicated that the levels of polyamines remain unchanged or significantly decrease in plantlets treated with endophytic bacteria after A. tumefaciens challenge compared to the control and plantlets treated with individual endophytic bacterial strains. PR1, PR2, and PR4 gene expression levels of plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 significantly increased after A. tumefaciens inoculation. The findings revealed the efficacy of the selected endophytic bacteria in triggering grapevine resistance against A. tumefaciens and the possible use of these strains as an alternative to chemical control methods in grapevine crown gall disease management.

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Pathogenesis-Related Proteins in Grape

Cited by 14 publications

References 69 publications

Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)

Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)

Endophytic Beauveria bassiana activates expression of defence genes in grapevine and prevents infections by grapevine downy mildew Plasmopara viticola

Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine

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