Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine

Abstract

Crown gall disease of grapevine, caused by Agrobacterium tumefaciens, often results in severe economic loss to grape production worldwide. This study demonstrated the ability of the endophytic bacteria Pseudomonas sp. Sn48 and Pantoea sp. Sa14 isolated from domesticated and wild grapevines to induce resistance in both above‐ and belowground tissues of grapevines infected with A. tumefaciens. Our results provide evidence that both strains can colonize roots and/or shoots. We showed that the strains Pseudomonas sp. Sn48 and Pantoea sp. Sa14 are capable of inducing stilbenic phytoalexin production in grapevine tissues and to further prime plantlets for enhanced phytoalexin production after A. tumefaciens inoculation. We also showed that in the majority of treatments, polyamine accumulation remained unchanged or slightly increased in plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 compared with the control. Our findings indicated that the levels of polyamines remain unchanged or significantly decrease in plantlets treated with endophytic bacteria after A. tumefaciens challenge compared to the control and plantlets treated with individual endophytic bacterial strains. PR1, PR2, and PR4 gene expression levels of plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 significantly increased after A. tumefaciens inoculation. The findings revealed the efficacy of the selected endophytic bacteria in triggering grapevine resistance against A. tumefaciens and the possible use of these strains as an alternative to chemical control methods in grapevine crown gall disease management.

Calcium oxide, potassium phosphite and a Trichoderma enriched compost water suspension protect Capsicum annuum against Phytophthora capsici by priming the immune system

“…While acibenzolar-s-methyl treatment was able to overexpress CaDEF1 gene in accordance with Zhang et al, 32 where P. capsici induced an overexpression of the defensin gene in pepper 24 h after the inoculation. Moreover, compost water suspension treated plants overexpressed CaPBR1 gene at T24 in accordance with Asghari et al, 40 and Esmail et al, 41 who demonstrated overexpression of the target gene 24 h after inoculation respectively in: grapevine plants against Agrobacterium tumefaciens and in Triticum aestivum plants primed with diverse Trichoderma strains against Puccinia graminis f. sp. tritici.…”

Endophytic Bacteria From the Roots of the Medicinal Plant Alkanna tinctoria Tausch (Boraginaceae): Exploration of Plant Growth Promoting Properties and Potential Role in the Production of Plant Secondary Metabolites

“…In addition, some microbes can induce plant defenses. Asghari et al (2020) demonstrated that Pseudomonas sp. Sn48 and Pantoea sp.…”

Resistance analysis of cherry rootstock ‘CDR-1’ (Prunus mahaleb) to crown gall disease

“…Crown gall disease was identified long ago as a bacterial plant disease [1], and its pathogenic bacterium is Agrobacterium tumefaciens, which mainly infects dicots. This disease often results in severe economic losses to the production of cherry and other fruit trees [2][3][4]. Crown gall disease starts with the attachment of A. tumefaciens to plant cell.…”