Pathogenesis of swine influenza virus (Thai isolates) in weanling pigs: an experimental trial

Sreta, Donruethai; Kedkovid, Roongtham; Tuamsang, Sophon; Kitikoon, Pravina; Thanawongnuwech, Roongroje

doi:10.1186/1743-422x-6-34

Cited by 52 publications

(58 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thai SIV subtype H1 can be further divided into 3 clusters (clusters I-III). 30,31,34 Therefore, whole genome characterization of previous and current Thai SIV isolates is necessary because it could provide essential epidemiologic data and diagnostic and disease preparedness of the current viruses. Currently in Thailand, there are only 16 whole genomes of 2000-2005 (6 H1N1, 9 H3N2, and 1 H1N2), 8 H1N1 whole genomes of 2009-2010, 20,31,34 and 38 HA genes available in the public database.…”

Section: Hemagglutination Inhibitionmentioning

confidence: 99%

“…2 Interestingly, in Thailand, the reassortant H1N1 SIV between the North American and Eurasian lineage has been reported as swine breeds have been continuingly imported from both continents. 30,34 Similarly, antigenic and genetic diversity of swine H1N1 and H1N2 viruses in Europe have been documented. 19 This indicates that a constant update of the representative filed strain virus within different regions of the world is required to reflect true SIV serologic data.…”

Section: Introductionmentioning

confidence: 99%

“…Direct detection of SIV and/or viral genetic materials, however, can be problematic because the duration of SIV shedding in infected pigs is short. 26,30 Thus, SIV-specific antibody detection used as an indicator of previous SIV exposure can become helpful particularly in areas where SIV vaccination is not commonly performed, such as in Thailand. Because no SIV vaccines are currently used in the Thai swine population, SIV-specific antibody detected in pigs, therefore, reflects SIV infection or previous exposure to the specific virus.…”

Section: Introductionmentioning

confidence: 99%

“…Three major subtypes including H1N1, H3N2, and H1N2 are currently circulating worldwide in swine populations, 2 including Thailand. 4,30,34 Swine influenza virus (SIV) infection causes acute respiratory syndrome including cough, dyspnea, fever, and prostration with rapid recovery. 30 The morbidity rate of SIV is high and may increase up to 100% in immunologically naïve pig populations whereas the mortality rate can be as low as 1%.…”

Section: Introductionmentioning

confidence: 99%

“…4,30,34 Swine influenza virus (SIV) infection causes acute respiratory syndrome including cough, dyspnea, fever, and prostration with rapid recovery. 30 The morbidity rate of SIV is high and may increase up to 100% in immunologically naïve pig populations whereas the mortality rate can be as low as 1%. 2 Infected pigs will lose weight, resulting in an increased growing period to normalize weight before going to the slaughterhouse.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Retrospective swine influenza serological surveillance in the four highest pig density provinces of Thailand before the introduction of the 2009 pandemic Influenza A virus subtype H1N1 using various antibody detection assays

et al. 2012

Self Cite

View full text Add to dashboard Cite

Genetic characterization of the hemagglutinin gene of the 6 selected Thai Swine influenza virus (SIV) isolates (4 H1 and 2 H3 isolates) used in the establishment of a hemagglutination inhibition (HI) assay was analyzed. Based on the phylogenetic analysis, Thai SIVs could be divided into 3 clusters of the H1 viruses (clusters I and II belonging to classical swine H1α, and cluster III belonging to classical swine H1γ), and 2 clusters of the H3 viruses both belonging to human-like 1970s. The serological results indicated that swH1N1-06 (H1 cluster I) is a suitable representative SIV for the HI test antigen to detect H1 SIV-specific antibodies in the Thai swine population, while both swH3N2-05 and swH3N2-07 should be used for Thai H3 SIV-specific antibody detection. The HI test results of swine sera collected from pigs in the 4 highest pig population provinces of Thailand indicated that the percentage of pigs seropositive to swH3N2-07 was highest compared to swH1N1-06, swH1N1-09, and swH3N2-05 (85.4%, 50.1%, 18.6%, and 15.8%, respectively). It should be noted that countries lacking SIV genetic information should be concerned with determining the most suitable HI test antigens to use when performing the tests due to the genetic variation and limited cross-reaction of SIVs. The results of the current study demonstrated that HI tests should be implemented with the suitable field strains as the representative test antigen to ascertain accurate SIV serostatus in Thailand and that test antigens should be genetically analyzed and compared with circulating strains regularly.

show abstract