Pathogenesis of Escherichia Coli O157:H7 in Weaned Calves

Dean-Nystrom, Evelyn A.; Bosworth, Brad T.; Moon, Harley W.

doi:10.1007/978-1-4615-4143-1_16

Cited by 68 publications

(81 citation statements)

References 7 publications

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“…This lack of enterotoxicity may be due to the lack of Stx binding to enterocytes and blood vessels within the calf ileum. In aggregate, the results are consistent with prior evidence suggesting that Stx is not required for E. coli O157:H7 to be pathogenic in neonatal calves (38).…”

Section: Discussionsupporting

confidence: 82%

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Pruimboom‐Brees

Morgan

Ackermann

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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175

143

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Escherichia coli O157:H7 causes Shiga toxin (Stx)-mediated vascular damage, resulting in hemorrhagic colitis and the hemolytic uremic syndrome in humans. These infections are often foodborne, and healthy carrier cattle are a major reservoir of E. coli O157:H7. We were interested in knowing why cattle are tolerant to infection with E. coli O157:H7. Cattle tissues were examined for the Stx receptor globotriaosylceramide (Gb3), for receptivity to Stx binding in vitro, and for susceptibility to the enterotoxic effects of Stx in vivo. TLC was used to detect Gb3 in tissues from a newborn calf. Gb3 was detected by TLC in kidney and brain, but not in the gastrointestinal tract. Immunohistochemistry was used to define binding of Stx1 and Stx2 overlaid onto sections from cattle tissues. Stx1 and Stx2 bound to selected tubules in the cortex of the kidney of both newborn calves (n ‫؍‬ 3) and adult cattle (n ‫؍‬ 3). Stx did not bind to blood vessels in any of the six gastrointestinal and five extraintestinal organs examined. The lack of Gb3 and of Stx receptivity in the gastrointestinal tract raised questions about the toxicity of Stx in bovine intestine. We found that neither viable E. coli O157:H7 nor Stx-containing bacterial extracts were enterotoxic (caused fluid accumulation) in ligated ileal loops in newborn calves. The lack of vascular receptors for Stx provides insight into why cattle are tolerant reservoir hosts for E. coli O157:H7.

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Section: Discussionsupporting

confidence: 82%

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Pruimboom‐Brees

Morgan

Ackermann

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

175

143

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“…Grauke et al (2002) noted a correlation between positive fecal samples and isolation from the rumen and duodenum while Naylor et al (2003) found the primary site of infection in the recto-anal junction. Other reports have confirmed the rectum and cecum as primary sites of infection in cattle (Buchko et al, 2000;Dean-Nystrom et al, 1999). These findings suggest that oral bacteriophage treatment, with bacteriophages selected for anaerobic activity, may affect E. coli O157:H7 colonization in sheep and cattle.…”

Section: Successessupporting

confidence: 60%

Successes and Failures of Bacteriophage Treatment of Enterobacteriaceae Infections in the Gastrointestinal Tract of Domestic Animals

Bielke¹,

Téllez²,

Hargis³

2012

Bacteriophages

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“…The bacterium may persist in cattle for many months, but generally persists for about 30 days or less [20,21]. In experimental infections, DeanNystrom et al [22,23] showed that with a dose of 10 10 cfu of a ®ve-strain mixture of EHEC O157:H7, normal neonatal calves of 12±36 h of age gave attaching and effacing lesions in both small and large intestines and lesions were detectable in the rectum of 3-month-old weaned calves. These data indicate that the mechanisms for intimate attachment encoded by the locus of enterocyte effacement of EHEC O157:H7 do mediate mucosal colonisation in bovines.…”

Section: Introductionmentioning

confidence: 99%

An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures

Dibb-Fuller¹,

Best

Stagg³

et al. 2001

Journal of Medical Microbiology

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Sections of kidney, trachea, ileum, colon, rectum and rumen were removed at post mortem from a neonatal calf and, with the exception of the rumen, primary cell lines were established for each of the cell types. The adherence of enterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7, enteropathogenic E. coli (EPEC) serotype O111, E. coli K12 (a laboratory adapted non-pathogenic strain) and Salmonella enterica serotype Typhimurium was assayed on each cell type. For all adherence assays on all cell lines, EHEC O157:H7 adhered to a signi®cantly greater extent than the other bacteria. S. Typhimurium and EPEC O111 adhered to a similar extent to one another, whereas E. coli K12 was signi®cantly less adherent by 100-fold. In all cell types, >10% of adherent S. Typhimurium bacteria invaded, whereas c. 0.01±0.1% of adherent EHEC O157:H7 and EPEC O111 bacteria invaded, although they are regarded as non-invasive. EHEC O157 generated actin re-arrangements in all cell types as demonstrated by¯uorescent actin staining (FAS) under densely packed bacterial micro-colonies. EPEC O111 readily generated the localised adherent phenotype on bovine cells but generated only densely packed micro-colonies on HEp-2 cells. The intensity of actin re-arrangements induced in bovine cells by EPEC O111 was less than that induced by EHEC O157:H7. The intimate attachment on all cell types by both EHEC O157:H7 and EPEC O111 was clearly demonstrated by scanning electron microscopy.

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Pathogenesis of Escherichia Coli O157:H7 in Weaned Calves

Cited by 68 publications

References 7 publications

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins

Successes and Failures of Bacteriophage Treatment of Enterobacteriaceae Infections in the Gastrointestinal Tract of Domestic Animals

An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures

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